Effects of DL-methionine and a methionine hydroxy analogue (MHA-Ca) on growth, amino acid profiles and the expression of genes related to taurine and protein synthesis in common carp (Cyprinus carpio)

Zhou, Yuanyuan; He, Jia; Su, Ningning; Masagounder, Karthik; Xu, Mingyuan; Chen, Leling; Liu, Qingying; Ye, Huaqun; Sun, Zhenzhu; Ye, Chaoxia

doi:10.1016/j.aquaculture.2020.735962

Cited by 17 publications

(10 citation statements)

References 54 publications

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“…DL-Met supplementation improved feed conversion and protein efficiency ratios when compared with the basal diet (REF), while MHA fed fish presented intermediate results. These results are in agreement with previous studies in fish (13,16,17,59) , demonstrating that DL-Met supplementation improves growth and promotes protein accretion more efficiently in Nile tilapia.…”

Section: Discussionsupporting

confidence: 93%

“…In rainbow trout, dietary MHA-Ca salt was found to have lower bioavailability than DL-Met, resulting in 69 % lower fish weight gain, 60 % lower growth rate and 73 % lower N retention in fish (16) . Similarly, growth performance and feed utilisation indicators in common carp (Cyprinus carpio) fed DL-Met and MHA-Ca supplemented diets demonstrated that MHA-Ca was 41-50 % as available as DL-Met on weight-for-weight basis (17) . In contrast, some authors report similar efficiencies among Met sources based on growth and feed efficiency in several aquatic animals (18)(19)(20)(21) .…”

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confidence: 96%

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Metabolic and nutritional responses of Nile tilapia juveniles to dietary methionine sources

et al. 2021

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Commercial diets for tilapia juveniles contain high levels of plant protein sources. Soybean meal has been utilised due to its high protein content, however, soy-based diets are limited in methionine and require its supplementation to fulfil fish requirements. DL-methionine (DL-Met) and calcium bis-methionine hydroxyl analogue (MHA-Ca) are synthetic methionine sources supplemented in aquafeeds, which may differ in biological efficiency due to structural differences. The present study evaluated the effect of both methionine sources on metabolism and growth of Nile tilapia. A growth trial was performed using three isonitrogenous and isoenergetic diets, containing plant ingredients as protein sources: DLM and MHA diets were supplemented on equimolar levels of methionine, while REF diet was not supplemented. Hepatic free methionine and one-carbon metabolites were determined in fish fed for 57 days. Metabolism of DL-Met and MHA was analysed by an in vivo time-course trial using 14C-labelled tracers. Only DL-Met supplementation significantly increased final body weight and improved feed conversion and protein efficiency ratios compared to the REF diet. Our findings indicate that methionine in DLM fed fish follows the transsulfuration pathway while in fish fed MHA and REF diets it is remethylated. The in vivo trial revealed that 14C-DL-Met is absorbed faster and more retained than 14C-MHA, resulting in a greater availability of free methionine in the tissues when fish is fed with DLM diet. Our study indicates that dietary DL-Met supplementation improves growth performance and nitrogen retention, and that methionine absorption and utilisation is influenced by the dietary source in tilapia juveniles.

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Section: Discussionsupporting

confidence: 93%

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confidence: 96%

Metabolic and nutritional responses of Nile tilapia juveniles to dietary methionine sources

et al. 2021

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“…In fish, knowledge on the efficacy–efficiency of different Met sources on growth performance and metabolism is scarce. In species such as rainbow trout, common carp, Nile tilapia, channel catfish, and cobia, DL-Met resulted a better source than MHA with regard to the growth performance [ 13 , 15 , 16 , 17 ]. In rainbow trout, DL-MHA calcium salt was less bioavailable (60–73%) in comparison to DL-Met based on several performance parameters, such as weight gain, growth rate, and retained nitrogen [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?

Ceccotti

Biasato

Gasco

et al. 2022

CIMB

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In aquafeeds in which plant proteins are used to replace fishmeal, exogenous methionine (Met) sources are demanded to balance the amino acid composition of diets and meet the metabolic fish requirements. Nonetheless, since different synthetic Met sources are commercially available, it is important to determine their bioavailability and efficacy. To address this issue, we conducted a two-month feeding trial with rainbow trout (Oncorhynchus mykiss), which were fed diets supplemented with five different forms of Met: Met-Met, L-Met, HMTBa, DL-Met, and Co DL-Met. No differences in growth performance were found in trout fed with different Met forms, but changes in the whole-body composition were found. In particular, Met-Met and L-Met promoted a significant body lipid reduction, whereas the protein retention was significantly increased in fish fed with HMTBa and Co DL-Met. The latter affected the hepatic Met metabolism promoting the trans-sulfuration pathway through the upregulation of CBS gene expression. Similarly, the L-Met enhanced the remethylation pathway through an increase in BHMT gene expression to maintain the cellular demand for Met. Altogether, our findings suggest an optimal dietary intake of all tested Met sources with similar promoting effects on fish growth and hepatic Met metabolism. Nevertheless, the mechanisms underlying these effects warrant further investigation.

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“…For many other animals like mammals, birds and, also, specific fish, the equimolar bioefficacy of MHA in comparison to DL-MET has been shown to be only around 74%. These differences in bioavailability between these two forms were previously established, with differences in intestinal fluxes recently proposed [ 6 , 7 ] as a likely explanation; however, questions still remain to be elucidated with respect to the outcomes of these differences in other tissues. To address this question, RT liver-derived cell lines were used, as a simplified model, to extrapolate the unique and specific effects of DL-MET or MHA on RT liver functions.…”

Section: Discussionmentioning

confidence: 86%

“…Nonetheless, and beyond the fact that both sources can be used as a MET source, knowledge about fish performances are scarce and still debated when directly comparing their efficiencies. For instance, while studies performed in rainbow trout [ 4 ], sunshine bass [ 5 ], common carp [ 6 ], Nile tilapia [ 7 ] and channel catfish [ 8 ]) showed that DL-Met is a better MET source than MHA with respect to their growth performances, other studies (e.g., in turbot [ 9 ] or channel catfish [ 10 ]) concluded that MHA is used as efficiently or even better than L-MET or DL-MET. Many hypotheses could explain these opposite conclusions, such as differences in diet compositions, concentrations used in relation to the Met requirement of the species and product forms used, as well as the species studied, but none of these studies investigated the molecular mechanisms that drive the effectiveness of a said supplementation compared to another.…”

Section: Introductionmentioning

confidence: 99%

Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions

Pinel

Héraud

Morin

et al. 2022

IJMS

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The replacement of fishmeal by plant proteins in aquafeeds imposes the use of synthetic methionine (MET) sources to balance the amino acid composition of alternative diets and so to meet the metabolic needs of fish of agronomic interest such as rainbow trout (RT-Oncorhynchus mykiss). Nonetheless, debates still exist to determine if one MET source is more efficiently used than another by fish. To address this question, the use of fish cell lines appeared a convenient strategy, since it allowed to perfectly control cell growing conditions notably by fully depleting MET from the media and studying which MET source is capable to restore cell growth/proliferation and metabolism when supplemented back. Thus, results of cell proliferation assays, Western blots, RT-qPCR and liquid chromatography analyses from two RT liver-derived cell lines revealed a better absorption and metabolization of DL-MET than DL-Methionine Hydroxy Analog (MHA) with the activation of the mechanistic Target Of Rapamycin (mTOR) pathway for DL-MET and the activation of integrated stress response (ISR) pathway for MHA. Altogether, the results clearly allow to conclude that both synthetic MET sources are not biologically equivalent, suggesting similar in vivo effects in RT liver and, therefore, questioning the MHA efficiencies in other RT tissues.

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Effects of DL-methionine and a methionine hydroxy analogue (MHA-Ca) on growth, amino acid profiles and the expression of genes related to taurine and protein synthesis in common carp (Cyprinus carpio)

Cited by 17 publications

References 54 publications

Metabolic and nutritional responses of Nile tilapia juveniles to dietary methionine sources

Metabolic and nutritional responses of Nile tilapia juveniles to dietary methionine sources

How Different Dietary Methionine Sources Could Modulate the Hepatic Metabolism in Rainbow Trout?

Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions

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