Effects of docosahexaenoic acid on the survival and neurite outgrowth of rat cortical neurons in primary cultures

Cao, Dehua; Xue, Rongjian; Xu, Jun; Liu, Zhili

doi:10.1016/j.jnutbio.2005.02.002

Cited by 102 publications

(69 citation statements)

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“…Recent studies have suggested that (n-3) fatty acid deficiency decreases the mean cell body size of neurons in the hippocampus, hypothalamus, and parietal cortex, and decreases the complexity of dendritic arborizations on cortical neurons (39,40). Consistent with this line of investigation, DHA enhanced neurite outgrowth of hippocampal and cortical neurons and rat cloncal pheochromocytoma (PC12) cells in culture (28,41,42). However, the usual caution is needed in extrapolating from studies with isolated cell types and transformed cells to the in vivo situation.…”

Section: Introductionmentioning

confidence: 66%

Dietary (n-3) Fatty Acids and Brain Development1

Innis

2007

The Journal of Nutrition

735

512

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Section: Introductionmentioning

confidence: 66%

Dietary (n-3) Fatty Acids and Brain Development1

Innis

2007

The Journal of Nutrition

735

512

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“…GAP-43 is a regeneration-associated protein that is critical for successful regeneration and has been shown to be increased by -3 PUFA in vitro (Cao et al, 2005). GAP-43 can be upregulated in both neurons and Schwann cells and, after the crush injury, was seen to be increased in the DRG and sciatic nerve.…”

Section: Discussionmentioning

confidence: 99%

Improved Outcome after Peripheral Nerve Injury in Mice with Increased Levels of Endogenous Omega-3 Polyunsaturated Fatty Acids

Gladman¹,

Huang²,

Lim³

et al. 2012

J. Neurosci.

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Functional recovery after a peripheral nerve injury (PNI) is often poor. There is a need for therapies that protect neurons against injury and enhance regeneration. Omega-3 polyunsaturated fatty acids (PUFAs) have been shown to have therapeutic potential in a variety of neurological disorders, including acute traumatic injury. The objective of this study was to assess the neuroprotective and proregenerative potential of -3 PUFAs in PNI. We investigated this in mice that express the fat-1 gene encoding for -3 fatty acid desaturase, which leads to an increase in endogenous -3 PUFAs and a concomitant decrease in -6 PUFAs. Dorsal root ganglion (DRG) neurons from wild-type or fat-1 mice were subjected to a mechanical strain or hypoxic injury, and cell death was assessed using ethidium homodimer-1 labeling. The fat-1 background appears to confer robust neuroprotection against both injuries. We then examined the early functional and morphological changes in wild-type and fat-1 mice after a sciatic nerve crush. An accelerated functional recovery 7 d after injury was seen in fat-1 mice when assessed using von Frey filaments and the sciatic nerve functional index. These observations were also mapped to changes in injury-related markers. The injury-induced expression of ATF-3 was decreased in the DRG of fat-1 mice, whereas the axons detected 6 mm distal to the crush were increased. Fat-1 animals also had some protection against muscle atrophy after injury. In conclusion, both in vitro and in vivo experiments support the idea that a higher endogenous -3 PUFA could lead to beneficial effects after a PNI.

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“…11,12 More specifically, we synthesized N-acylated tryptamine derivatives and screened these compounds using a cell culture system that models DA cell degeneration in PD. This resulted in the discovery of compounds having interesting anti-oxidative and neuroprotective/neuritogenic properties with, however, poor blood-brain barrier (BBB) permeability.…”

Section: Introductionmentioning

confidence: 99%