Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

Yamaguchi, Hiroyasu; Shimizu, Akifumi; Degi, Konosuke; Morishita, Toshikazu

doi:10.1270/jsbbs.58.331

Cited by 77 publications

(44 citation statements)

References 12 publications

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“…The latter group provoked very severe affectations in the material evaluated. These results agree with what was indicated by Yamaguchi et al (2008), who indicated that with low doses of radiation (2-10 Gy), less damage is produced on the genome of the plant.…”

Section: Classification Of the Doses By Cluster Analysissupporting

confidence: 92%

“…Este último grupo provocó afectaciones muy severas en el material evaluado. Estos resultados concuerdan con lo indicado por Yamaguchi et al (2008), quienes indican que con bajas dosis de radiación (2-10 Gy) se produce un daño menor en el genoma de la planta. Los resultados expuestos corroboran que la radiosensibilidad en las plantas varía de acuerdo a las dosis de irradiación absorbida, entre otros factores.…”

Section: L a S I F I C A C I ó N D E L A S D O S I S P O R A N á L unclassified

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EFFECT OF GAMMA RADIATION ON Abies religiosa (Kunth) Schltd. et Cham

Iglesias-Andreu¹,

Sánchez-Velásquez²,

Tivo-Fernández³

et al. 2010

rchscfa

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Section: Classification Of the Doses By Cluster Analysissupporting

confidence: 92%

Section: L a S I F I C A C I ó N D E L A S D O S I S P O R A N á L unclassified

EFFECT OF GAMMA RADIATION ON Abies religiosa (Kunth) Schltd. et Cham

Iglesias-Andreu¹,

Sánchez-Velásquez²,

Tivo-Fernández³

et al. 2010

rchscfa

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“…Yamaguchi et al (2008) Ionizing radiation can be quantified in terms of absorbed dose (D) which is the amount of ionizing radiation energy deposited per unit mass of irradiated material. Initially, the magnitude was measured in roentgens, called "radiation dose".…”

Section: 30 and 60 Gymentioning

confidence: 99%

Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species

Iglesias-Andreu¹,

Octavio‐Aguilar²,

Bello-Bello³

2012

Gamma Radiation

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“…The gain or loss of a single chromosome can be detected by this method, as shown in Musa (Roux et al 2003), ryegrass (Barker et al 2001), and wheat-rye addition lines (Bashir et al 1993, Pfosser et al 1995. Moreover, Yamaguchi et al (2008) reported that nuclear DNA content of chrysanthemums decreased with increasing dose and dose rate of gamma rays, indicating that the nuclear DNA content could be used as an index of radiation damage.…”

Section: Introductionmentioning

confidence: 98%

Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

et al. 2010

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We investigated the effects of ion beams, in comparison to gamma rays, on mutation induction and nuclear DNA content as an index of radiation damage in Chrysanthemum morifolium. Leaf segments were irradiated with 220 MeV carbon ions (mean linear energy transfer = 107 keV/µm), 320 MeV carbon ions (76 keV/µm), 100 MeV helium ions (9 keV/µm), and gamma rays. The nuclear DNA content was measured and frequency of flower color mutations was investigated in regenerated plants. The number of plants with reduced nuclear DNA content increased with increasing irradiation doses of 320 MeV carbon ions, 100 MeV helium ions, and gamma rays. In contrast, they did not increase with 220 MeV carbon ions, even when the dose was increased. Irradiation treatment with 220 and 320 MeV carbon ions and gamma rays had a similar effect on mutation induction, while the effect of 100 MeV helium ions was not as great. Thus, the effects of irradiation treatments on mutation induction and nuclear DNA content differed according to the type of ion beams. 220 MeV carbon ion beam seemed to be the most appropriate among the three types of ion beams because it gave a high mutation frequency with low damage to chromosomes.Key Words: chrysanthemum, flower color mutation spectrum, gamma rays, ion beams, mutation frequency, nuclear DNA content. IntroductionChrysanthemum is a widely cultivated ornamental plant worldwide. Mutation is an important tool in chrysanthemum breeding. Many mutant cultivars have been produced by spontaneous mutations (sports) and induced artificially by gamma or X-rays. In Japan, ion beams that consist of ion particles, accelerated by a cyclotron, have been used to attempt to induce mutations since the 1990s. Ion beams have high linear energy transfer (LET), and thus, greater biological effects compared to low LET radiation such as gamma and X-rays. Thus, ion beams are expected to produce higher mutation frequencies and novel mutants. It has been demonstrated that ion beams induce mutations at high frequency and produce broad mutation spectrum, including novel mutants, in Arabidopsis Hase et al. 2000, Tanaka et al. 1997. Recently, mutation induction by ion beam irradiation has been carried out in various plants, particularly ornamental plants such as carnation (Okamura et al. The use of ion beams for inducing mutations in chrysanthemum breeding has also been attempted (Furutani et al. 2008, Nagatomi et al. 1998, Shirao et al. 2007, Suzuki et al. 2005, Ueno et al. 2005, Wakita et al. 2008, Watanabe et al. 2008. Thus, ion beam-specific flower color mutants that could not be obtained using gamma rays were obtained by the abovementioned programs (Nagatomi et al. 1998 Although radiation treatment induces mutations, it causes radiation damage. Konzak et al. (1965) suggested that not only mutation induction effect but also the plant damage caused by irradiation treatment, such as chromosomal aberrations, should be considered for the use of any mutagen in plant breeding. Reduction in chromosome number was reported in chrysanthemums irrad...

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Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

Cited by 77 publications

References 12 publications

EFFECT OF GAMMA RADIATION ON Abies religiosa (Kunth) Schltd. et Cham

EFFECT OF GAMMA RADIATION ON Abies religiosa (Kunth) Schltd. et Cham

Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species

Effects of ion beam irradiation on mutation induction and nuclear DNA content in chrysanthemum

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