Effects of Dose of Recombinant Human BMP-2 on Bone Formation at Palatal Sites in Young and Old Rats

Yamaji, Kozo; Kawanami, Masamitsu; Matsumoto, Atsushi; Odajima, Tomoomi; Nishitani, Yosuke; Iwasaka, Kensuke; Yoshimitsu, Keiko; Yoshiyama, Masahiro

doi:10.4012/dmj.26.481

Cited by 13 publications

(8 citation statements)

References 23 publications

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“…BMPs) which have osteogenic and osseoconductive effects (Rahn and Perren, 1970;Rahn, 1976;Allegrini et al, 2003). The osseoinductive effect of rhBMP-2 depends on the amount of cells in the region of augmentation which in turn depends on the species, the age and the vascularity of the area (Terheyden et al, 1999;Yamaji et al, 2007). Sinus-lift procedures in a sheep model are a reliable method for evaluating bone formation (Aral et al, 2008;Estaca et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep

Gutwald

Haberstroh

Stricker

et al. 2010

Journal of Cranio-Maxillofacial Surgery

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SUMMARY.Background: Several studies have reported certain bone morphogenic proteins (BMPs) to have positive effects on bone generation. Although some investigators have studied the effects of human recombinant BMP (rhBMP-2) in sinus augmentation in sheep, none of these studies looked at the placement of implants at the time of sinus augmentation. Furthermore, no literature could be found to report on the impact that different implant systems, as well as the positioning of the implants had on bone formation if rhBMP-2 was utilized in sinus-lift procedures. Purpose: The aim of this study was to compare sinus augmentation with rhBMP-2 on a poly-D, L-lactic-co-glycolic acid gelatine (PLPG) sponge with sinus augmentation with autologous pelvic cancellous bone in the maxillary sinus during the placement of different dental implants. Materials and methods: Nine adult female sheep were submitted to bilateral sinus-floor elevation. In one side (test group) the sinus lift was performed with rhBMP-2 on a PLPG-sponge, while the contralateral side served as the control by using cancellous bone from the iliac crest. Three different implants (Bränemark Ò , 3i Ò and Straumann Ò ) were inserted either simultaneously with the sinus augmentation or as a two staged procedure 6 weeks later. The animals were sacrificed at 6 and 12 weeks for histological and histomorphometrical evaluations during which boneto-implant contact (BIC) and bone density (BD) were evaluated. Results: BD and BIC were significantly higher at 12 weeks in the test group if the implants were placed at the time of the sinus lift (p \ 0.05). No difference was observed between the different implant systems or positions. Conclusions: The use of rhBMP-2 with PLPG-sponge increased BIC as well as BD in the augmented sinuses if compared to autologous bone. Different implant systems and positions of the implants had no effect on BIC or BD. Ó 2010 European Association for Cranio-Maxillo-Facial Surgery

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Section: Discussionmentioning

confidence: 99%

Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep

Gutwald

Haberstroh

Stricker

et al. 2010

Journal of Cranio-Maxillofacial Surgery

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“…According to BMP‐2(100) group, the peak of its mineral content was observed at 4 weeks, whereas the mineral content of BMP‐2(50) group was maintained ascending to 12 weeks, which might suggest that the peak point was advanced as the dose of the plasmid increased. This might be because osteogenic reagents, such as BMP‐2, recruit and activate not only osteoprogenitor cells but also bone‐resorbing cells like osteoclasts 32–34. Since a fine balance probably exists between the opposing effects of osteogenic factors, it is necessary to optimize its dose, its mode of delivery, and the timing of its release to maximize osteoinductive or osteoconductive responses.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyapatite fiber material with BMP‐2 gene induces ectopic bone formation

Kuroda

Kondo

et al. 2008

J Biomed Mater Res

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Collagen containing bone morphogenetic protein-2 (BMP-2) expression vector, which is called "gene-activated matrix," promotes bone regeneration when transplanted to the bone defect. We speculated that hydroxyapatite fiber (HF) would be an ideal matrix for "gene-activated matrix" especially for bone regeneration, because it is oseteoconductive and has high affinity to DNA. The purpose of this study is to clarify whether HF containing BMP-2 expression vector induces ectopic bone formation. We prepared HF containing 0, 10, 50, and 100 microg BMP-2 expression vector. Wistar male rats (8 weeks) were used and each rat received two HF implants in the left and right dorsal muscle. The rats were sacrificed 4, 8, and 12 weeks after the operation, and implants were analyzed radiographically by softex, dual-energy X-ray absorptiometry, and they were histologically examined. At 4 weeks, HF containing 50 or 100 microg BMP-2 expression vector showed high bone mineral contents and large radiopaque volume compared to the other implants. At 8 and 12 weeks, HF containing 50 microg BMP-2 expression vector exerted the highest values in the radiographic analyses. Bonelike tissue was histologically observed in HF containing 50 and 100 microg BMP-2 expression vector groups but not detected in the other implants. The present results suggest that HF is potential as a matrix for "gene-activated matrix" for bone tissue engineering.

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“…BMP2 and BMP7 homodimers were reported to accelerate bone formation efficiently. However, the use of the BMP homodimers is associated with a high cost 5,24) and potential side-effects 25) . BMP 2/7 heterodimers have been proposed to overcome the disadvantages of BMP homodimers.…”

Section: Discussionmentioning

confidence: 99%

“…Several regional bone regeneration techniques including bone tissue engineering has been reported to solve such peri-implant bone defect [1][2][3] . Bone morphogenetic proteins (BMPs) emerge as promising candidates for bone tissue engineering due to their significant effects on bone and cartilage growth 4,5) . The efficacy of BMP homodimers therapy has been proved to be successful in many animal experiments, but the wide application of BMP homodimers in the clinic is limited by the high effective dosage (e.g.…”

Section: Introductionmentioning

confidence: 99%

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

Sun

Wang

Zheng

et al. 2012

Dental Materials Journal

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This study aimed to compare the effects of bone morphogenetic protein BMP2/7 heterodimer and BMP homodimers on bone regeneration in bone defects model. Identical peri-implant bone defects model were created using proper controls on the frontal skull in 18 minipigs. Collagen sponges with low-dose (30 ng/mL) BMP2/7 heterodimer, BMP2 or BMP7 homodimer were filled in the defects. New bone formation and the expression of type I collagen (Col1), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated after 2, 3, and 6 weeks of implantation. BMP2/7 resulted in significantly higher new bone areas percentage in the defect region than BMP2 and BMP7 (p<0.05). Immunohistochemical staining of Col1, ALP and OCN was stronger in BMP2/7 group than BMP2, BMP7 and control group (p<0.05). These results demonstrate that BMP2/7 heterodimer is a stronger inducer of osteoblastogenesis and could be applied at low dose to reduce the cost and side effects of BMP homodimers.

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Effects of Dose of Recombinant Human BMP-2 on Bone Formation at Palatal Sites in Young and Old Rats

Cited by 13 publications

References 23 publications

Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep

Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep

Hydroxyapatite fiber material with BMP‐2 gene induces ectopic bone formation

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

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