Effects of drinking water acidification by organic acidifier on growth performance, digestive enzyme activity and caecal bacteria in growing rabbits

Zhu, Keyong; Xu, Xiu Rong; Sun, D.F.; Tang, J.L.; Zhang, Yukun

doi:10.1016/j.anifeedsci.2014.01.014

Cited by 25 publications

(21 citation statements)

References 34 publications

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“…The proportion of methanogen or NC10 in total bacteria was detected by relative quantification real-time PCR (Zhu et al 2014), therefore there was no unit for this index. Values with different lowercase superscripts in the same row differ significantly ( P < 0.05)…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Nitrate decreases methane production also by increasing methane oxidation through stimulating NC10 population in ruminal culture

Liu

Cao

et al. 2017

AMB Expr

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Studies proved that addition of nitrate in rumen could lead to reduction of methane emission. The mechanism of this function was involved in the competition effect of nitrate on hydrogen consumption and the inhibitory effect of generated nitrite on methanogen proliferation. The present study investigated an alternative mechanism that denitrifying anaerobic methane oxidizing (DAMO) bacteria, DAMO archaea and anammox bacteria may co-exist in rumen, therefore, more methane can be oxidized when addition of nitrate. Ruminal batch culture model was used to test the effects of addition of 5 mM NaNO3, 4 mM NH4Cl, or both into the culture substrate on methane production, fermentation patterns, and population of methanogens, NC10 and anaerobic methanotrophic-2d (ANME-2d). Our results showed that NC10 in the ruminal culture was detected by polymerase chain reaction (PCR) when using NC10 special primer sets, and addition of nitrate reduced methane production and the relative proportions of methanogen, whereas increased the relative proportion of NC10. A combined addition of ammonia salt and nitrate did not show further inhibitory effect on methane production but accelerated nitrate removal. We did not detect DAMO archaea in ruminal culture by real-time PCR when using ANME-2d special primer sets. The present study may encourage researchers to pay more attention to methane oxidation performed by anaerobic methanotroph when studying the strategies of inhibiting ruminal methane emission.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0377-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…The relative proportion values were calculated using the 2 −ΔΔCt method as previously described (Zhu et al 2014). …”

Section: Methodsmentioning

confidence: 99%

Nitrate decreases methane production also by increasing methane oxidation through stimulating NC10 population in ruminal culture

Liu

Cao

et al. 2017

AMB Expr

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“…Gouet and Fonty (1979) reported that Lactobacillus was not detected in the intestine of rabbits by using the pure culture method, however, Zhu et al [28]showed that Lactobacillus was present in the cecum of rabbits by real-time quantitative PCR inspection. The cecal microflora of young rabbits has been established at weaning, but the species become more complicated with the increase of age at 3 weeks after weaning.…”

Section: Characteristics Of the Change Of Intestinal Flora After Weanmentioning

confidence: 99%

The Review on Structure of Intestinal Flora at Different Growth Stages of Rabbits

Chen¹,

Yang²,

Wang³

2018

dtbh

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“…Bacteroides-Prevotella, Escherichia coli and total bacteria Total bacterial genomic DNA of intestinal content was extracted by modified phenol-chloroform-isoamyl alcohol extraction method (Zhu et al, 2014). Concentration of the extracted DNA solution was subsequently determined, and then diluted to a concentration of 15 ng/μL.…”

Section: Real-time Polymerase Chain Reaction Assay For Determination mentioning

confidence: 99%

“…The creep feed for kits based on corn, alfalfa meal and soybean meal was formulated according to Zhu et al (2014). Ingredient and chemical composition of the diet are shown in Table 1.…”

Section: Experimental Dietmentioning

confidence: 99%

Effects of doe-litter separation on intestinal bacteria, immune response and morphology of suckling rabbits

Zhang

Cui

Sun³

et al. 2018

World Rabbit Sci.

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Gut development is stimulated by exposure to microorganisms, especially early-life microbial exposure. This study aimed to investigate whether doe-litter separation, which is performed in many rabbit farms, affects this exposure and therefore inhibits the development of intestinal system in suckling rabbits. Immediately after parturition, Rex rabbit does (n=16) were adjusted to 8 kits per litter and divided into doe-litter separation (DLS) group and doe-litter together (DLT) group based on the conditions of the does. One healthy kit per litter was selected and sacrificed at 7 d, 14 d, 21 d and 28 d of age, and the number of total bacteria, Escherichia coli and Bacteroides-Prevotella, expression of interleukin 6 (IL-6) and interleukin 10 (IL-10) in duodenum and caecum were investigated by real-time polymerase chain reaction. The morphological parameters of duodenum and vermiform appendix were also measured. Our results showed that doe-litter separation affected the number of intestinal bacteria. At 7 d of age, except for caecal Escherichia coli, the number of the investigated bacteria was decreased by doe-litter separation (P<0.05). But 1 wk later, only the number of total bacteria and Bacteroides-Prevotella in caecal content (P<0.05) and Escherichia coli in duodenal content from DLS kits (P<0.05) were still lower than those from DLT kits. After being provided with supplementary food for 7 d, DLS kits had fewer total bacteria in caecal content (P<0.05) and fewer E. coli in duodenal content (P<0.01) than DLT kits. After growing to 28 d of age, kits in DLS group still tended to have fewer total bacteria in caecal content, and expression of IL-10 and secretion of secretory IgA (sIgA) in vermiform appendix in DLS group was obviously lower than kits in DLT group (P<0.05). The villus height:crypt depth ratio in duodenum at 3 rd wk and 4 th wk was decreased by DLS (P<0.05). Kits in DLS group had shorter villus height (P<0.05), higher crypt depth (P<0.05) and shorter vermiform appendix (P<0.05) at the end of the trial. Furthermore, separating kits from the doe had a negative effect on their average daily gain at 3 rd wk and 4 th wk (P<0.05). Limiting the microbiological contact with the mother during suckling period affected the kits' intestinal flora and could negatively affect the development of intestinal digestive and immune system and growth performance of kits.

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Effects of drinking water acidification by organic acidifier on growth performance, digestive enzyme activity and caecal bacteria in growing rabbits

Cited by 25 publications

References 34 publications

Nitrate decreases methane production also by increasing methane oxidation through stimulating NC10 population in ruminal culture

Nitrate decreases methane production also by increasing methane oxidation through stimulating NC10 population in ruminal culture

The Review on Structure of Intestinal Flora at Different Growth Stages of Rabbits

Effects of doe-litter separation on intestinal bacteria, immune response and morphology of suckling rabbits

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