Effects of drought stress on physiological and biochemicalchanges in Phaseolus vulgaris L.

Kuşvuran, Şebnem; Daşgan, Hayriye

doi:10.18805/lr.v0i0.7025

Cited by 31 publications

(31 citation statements)

References 21 publications

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“…Consequently, one of the parameters that helped us to identify the degree of susceptibility to oxidative stress damage in bush bean cultivars was the determination of the MDA content, especially at the vegetative stage. It has been shown that the increase in the MDA concentration in Phaseolus vulgaris plants is related to traits of susceptibility to water deficit conditions as a result of the increased production of oxygen free radicals that generate oxidative stress in cells (Kusvuran and Dasgan, 2017). In this sense, our results suggest that cultivars Cerinza and Bacat a showed the highest levels of damage by lipid peroxidation of cell membranes under water deficit conditions at the vegetative stage ( Fig.…”

Section: Discussionsupporting

confidence: 54%

Drought-tolerant Common Bush Bean Physiological Parameters as Indicators to Identify Susceptibility

Sánchez-Reinoso¹,

Moreno²,

Restrepo-Díaz³

2019

horts

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Bean crops can be displaced to marginal areas or face abiotic stresses such as water deficit. Physiological responses allow the identification of tolerant genotypes and lead to more precise breeding strategies. The objective of this research was to evaluate the physiological (leaf gas exchange properties, leaf water content, and leaf thickness) and biochemical [proline and malondialdehyde (MDA)] responses of five common bush bean (Phaseolus vulgaris L.) cultivars (ICA-Cerinza, ICA-Bachue, NUA35, Bianca, and Bacatá) under a water shortage period by irrigation suspension (15 days) at two different phenological stages [vegetative: 40–55 days after seed emergence (DAE) or reproductive: (50–65 DAE)]. A completely randomized block design was carried out with a factorial arrangement (the phenological stage as the main factor and the cultivars as the secondary factor) for a total of 10 treatments with four repetitions per treatment. Leaf photosynthesis (Pn) showed equal photosynthesis values in control plants of all cultivars (≈20 μmol·m−2·s−1). The water deficit period reduced Pn close to 55% (≈12 μmol·m−2·s−1) at both, vegetative, or reproductive stage in all cases. Similar results were also observed on leaf thickness, with a reduction of ≈10% in water-stressed plants at either vegetative or reproductive stage in all evaluated cultivars. A higher MDA and proline production were observed in plants affected by a 15-day water deficit period, mainly at the vegetative stage. The obtained results suggest that the vegetative stage presented a more negative impact on the evaluated physiological variables in most of the cultivars used. Cultivar Bachue showed lower gas exchange properties affectation and higher proline content, which may indicate that this cultivar can be tolerant to water deficit stress conditions. This study allows suggesting that proline and MDA estimation are simple, fast, and low-cost techniques to screen cultivars to obtain more precise breeding selection in common bean. Finally, common bean cultivar selection through the use of biochemical markers can be complemented by the estimation of leaf gas exchange parameters at different phenological stages.

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Section: Discussionsupporting

confidence: 54%

Drought-tolerant Common Bush Bean Physiological Parameters as Indicators to Identify Susceptibility

Sánchez-Reinoso¹,

Moreno²,

Restrepo-Díaz³

2019

horts

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“…The obtained results showed that water deficit caused a reduction of the chlorophyll content in susceptible genotypes such as Bacata, whereas cultivar Bachue showed an increase in this variable. Reductions in the chlorophyll content have been reported in susceptible Phaseolus vulgaris L. genotypes to drought stress [35]. A reduction of chlorophyll under drought stress conditions is due to an overproduction of reactive oxygen species in the thylakoids [36].…”

Section: Discussionmentioning

confidence: 99%

Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean

Sánchez-Reinoso¹,

Moreno²,

Restrepo-Díaz³

2019

Agronomy

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The common bean is susceptible to drought conditions and the evaluation of plant responses to low water availability can be difficult. The quantification of chlorophyll fluorescence as a sensitive trait to environmental stresses is an important alternative in the characterization of drought-susceptible genotypes. The objective of this study was to evaluate mainly the use of chlorophyll α fluorescence (maximum efficiency of PSII (Fv/Fm), photochemical quenching (qP), non-photochemical quenching (NPQ)) and rapid light-response curves (RLCs) (initial slope of the curve (α), minimum saturation irradiance (Ik) and maximum relative electron transport rate (ETRmax)) parameters as tools for the identification of susceptible or tolerant bush bean cultivars to water deficit stress conditions in two different phenological stages. Using a randomized block design in a factorial arrangement, five bush bean cultivars (Cerinza, Bachue, NUA35, Bacata and Bianca) were evaluated under water deficit conditions by the suspension of irrigation for 15 days from 40 to 55 Days after Emergence (DAE) (vegetative stage) or 50 to 65 DAE (reproductive stage). The results showed that Fv/Fm and NPQ recorded the highest variation due to water deficit conditions, especially in the vegetative stage. The greatest reductions in Fv/Fm (0.67) and NPQ (0.71) were evidenced in cultivar NUA35 compared to its control plants (0.78 and 1.07, respectively). The parameters obtained from RLCs showed that cultivar Bacata registered the lowest α (0.17) and Ik (838.19 μmol∙m−2∙s−1) values compared to its control plants (α 0.23; Ik 769.99 μmol∙m−2∙s−1). Differences were only obtained in ETRmax in the reproductive stage (50–65 DAE) in which cultivar NUA35 reached values of 158.5 in stressed plants compared to control plants (251.22). In conclusion, the parameters derived from RLCs such as α and Ik can be used as tools to identify drought susceptibility in the vegetative stage, whereas ETRmax can be used in the reproductive stage. In addition, PSII photochemistry (Fv/Fm and NPQ) can also help to understand the agronomic responses of common bush bean cultivars to drought conditions.

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“…The chlorophyll content was higher in leaves with broader and dark green (E-25YG-Cu and E-50M50LB-Cu) leaves than the landraces with narrower leaves. This reflects that chlorophyll content increases with leaf growth and development but decreases at the senescence stage (Pereyra et al 2014) and under stress conditions such as drought (Kusvuran and Dasgan 2017).…”

Section: Variation In Morpho-agronomic Traitsmentioning

confidence: 99%

Variation on growth and yield traits among selected Phaseolus vulgaris landraces in KwaZulu-Natal, South Africa

Ndlangamandla

Ntuli

2019

Biodiversitas

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Abstract. Ndlangamandla VV, Ntuli NR. 2019. Variation on growth and yield traits among selected Phaseolus vulgaris landraces in KwaZulu-Natal, South Africa. Biodiversitas 20: 1597-1605. Phaseolus vulgaris L. (common bean) of American origin is grown worldwide for edible leaves, immature pods and dry seeds. This is the first comprehensive study conducted on variation among P. vulgaris landraces in South Africa. This study aimed to characterize variability in morpho-agronomic traits of P. vulgaris landraces. Twenty landraces were planted in a randomized complete block design with three replications. Variations in germination percentage as well as in stem, leaf, pod and seed traits were determined. Significant variations were recorded in all vegetative and reproductive traits except germination percentage and seed thickness. Vegetative traits correlated positively with each other, whereas reproductive traits correlated positively with both traits. Positive association of almost all traits with first and second components in a principal component analysis and biplot indicated them as potential discriminatory traits for landraces. The biplot and dendrogram associated landraces mainly according to their seed colour as well as growth and yield traits. This study revealed the potential vegetative and reproductive traits that can be used to select vigorously growing and high-yielding P. vulgaris landraces for future large-scale farming and breeding in South Africa. These traits could potentially result in desired plants with big stems, many branches, and numerous and broad leaves with high chlorophyll content, which will yield many, long and wide pods as well as many and heavy seeds. The taller landraces (D-50M50LB-Cl, D-50P50C-Cl, E-100YG-Cl, E-25YG-Cu, E-50M50LB-Cu, E-50B50C-Cl and E-50DB50C-Cl) from KwaZulu-Natal show good potential for future large-scale farming and breeding for vigorous growth as well as high pod and seed yield.

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Effects of drought stress on physiological and biochemicalchanges in Phaseolus vulgaris L.

Cited by 31 publications

References 21 publications

Drought-tolerant Common Bush Bean Physiological Parameters as Indicators to Identify Susceptibility

Drought-tolerant Common Bush Bean Physiological Parameters as Indicators to Identify Susceptibility

Chlorophyll α Fluorescence Parameters as an Indicator to Identify Drought Susceptibility in Common Bush Bean

Variation on growth and yield traits among selected Phaseolus vulgaris landraces in KwaZulu-Natal, South Africa

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