Effects of duodenal perfusion with sodium taurocholate on biliary and pancreatic secretion in man

Björnsson, Ó. G.; Maton, Paul N.; Fletcher, David; Chadwick, V. S.

doi:10.1111/j.1365-2362.1982.tb00945.x

Cited by 22 publications

(3 citation statements)

References 33 publications

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“…changes in pH (l, 9). Because indocyanine green is used s a marker to monitor hepato-biliary excretion into the duodenum (10)(11)(12), where pH and composition of the lumihal fluid is variable, we have in the present work studied the effects of storage and changes in pH on the spectro-photometric absorbance of indocyanine green in various biological fluids. The effects of high temperature on the rate of decay of indocyanine green in several organic solvents were also studied.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Studies on Indocyanine Green: Molar Lineic Absorbance, pH Tolerance, Activation Energy and Rate of Decay in Various Solvents

Björnsson¹,

Murphy²,

Chadwick³

et al. 1983

Clinical Chemistry and Laboratory Medicine

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Physicochemical studies were carried out on the tricarbocyanine dye indocyanine green in biological fluids and organic solvents. The molar lineic absorbance of the compound was highest in organic solvents (methanol, 1.2-propanediol, dimethylformamide) and bile, but lowest in water and duodenal fluid. Indocyanine green remained stable in methanol and bile (t 1/2 > l year) but was rapidly decomposed to a colourless derivative in duodenal fluid and distilled water (t 1/2 3.6 days and 1.4 days, respectively). It was thermostable (120 °C) in methanol and 1.2-propanediol but thermolabile in water and dimethylformamide where the activation energy for the decofnposition reaction was low. At ambient temperature (20 °C) indocyanine green was particularly labile at pH < 5 and pH > 11. The rate of decay of indocyanine green in various solvents indicated that the rate limiting Step in the decay process was either a first or zero order reaction.Physikochemische Untersuchungen an Indocyaningrün: Molare lineare Absorbanz, pH-Toleranz, Aktivierungsenergie und Zerfallsgeschwindigkeit in verschiedenen Lösungsmitteln Zusammenfassung: An dem Tricarbocyaninfarbstoff Indocyaningrün wurden physikochemische Untersuchungen in biologischen Flüssigkeiten und organischen Lösungsmitteln durchgeführt. Die molare lineare Absorbanz der Verbindung war am höchsten in organischen Lösungsmitteln (Methanol, 1,2-Propandiol, Dimethylformamid) und Galle, am. geringsten in Wasser und Duodenalsaft. Indocyaningrün war in Methanol und Galle stabil (tj/2 > l Jahr), wurde jedoch in Duodenalsaft und destilliertem Wasser sehr schnell zu einem farblosen Abkömmling zersetzt (ti/ 2 3,6 bzw. 1,4 Tage). Es war thermostabil (120 °C) in Methanol und 1,2-Propandiol, jedoch thefniolabil in Wasser und Dimethylformamid, in denen-die Aktivierungsenergie für die Zersetzungsreaktion niedrig war. Bei Umgebungstemperatur (20 °C) war der Farbstoff besonders labil bei pH<5 und pH> 11. Die Zerfallsgeschwindigkeit von Indocyaningrün in verschiedenen Lösungsmitteln zeigte, daß der Zerfallsprozeß eine Reaktionskinetik erster oder nullter Ordnung folgt.

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Section: Introductionmentioning

confidence: 99%

Physicochemical Studies on Indocyanine Green: Molar Lineic Absorbance, pH Tolerance, Activation Energy and Rate of Decay in Various Solvents

Björnsson¹,

Murphy²,

Chadwick³

et al. 1983

Clinical Chemistry and Laboratory Medicine

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“…All values are presented as the mean k SEM of the number of independent experiments shown in parentheses (n). The data were approximately normally distributed (frequency distribution), and no attempt was made to further normalize the data by logarithmic transformation before they were evaluated statistically by the paired or unpaired Student's t-test, as appropriate [12,13].…”

Section: Methodsmentioning

confidence: 99%

Regulation of VLDL secretion in primary culture of rat hepatocytes: involvement of cAMP and cAMP‐dependent protein kinases

Björnsson¹,

Sparks

et al. 1994

Eur J Clin Investigation

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When hepatocytes were cultured for 24 h in the presence of forskolin (10(-4) mol l-1) or isobutylmethylxanthine (IBMX, 10(-3) mol l-1), the intracellular cAMP concentration peaked (320-380 pmol mg-1 protein) after 10-20 min of culture. This increase was accompanied by a decrease in the secretion of triacylglycerol, cholesterol and apoprotein B associated with VLDL. After 4 h cAMP levels had returned almost to basal values but the inhibition of VLDL secretion persisted. There was a small intracellular accumulation of triacylglycerol but not of apoprotein B. Addition of forskolin and IBMX together led to a further increase in intracellular cAMP and a further suppression of VLDL output. Similar effects on the secretion of VLDL were also observed after addition of Bt2cAMP. Exposure of cell cultures to glucagon (10(-7) mol l-1) for only 10 min raised cellular cAMP levels to > 200 pmol mg-1 protein, and suppressed VLDL secretion during the next 24 h to < 40% of control. All of the substances tested inhibited de novo synthesis of fatty acids but had little or no effect on cholesterol synthesis and did not inhibit oleate esterification to triacylglycerol. The cAMP-dependent protein kinase antagonist Rp-cAMPS prevented suppression of VLDL triacylglycerol secretion induced by glucagon (10(-7) mol l-1) and abolished glucagon-induced ketogenesis. Rp-cAMPS also inhibited Bt2cAMP (7.5 x 10(-6) mol l-1)-induced suppression of VLDL secretion and enhancement of ketogenesis. It is concluded that rat hepatic VLDL metabolism can be regulated by cAMP and cAMP-dependent protein kinases, and that the initial transient rise in cellular cAMP levels induced by glucagon is sufficient to maintain a long-term inhibitory effect on assembly and secretion of VLDL.

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“…In some studies where bile acids were administered concomitantly with amino acids or fat, an inhibition of pancreatic enzyme secretion was observed. The mechanism underlying this observation is not completely understood, although it is possible that bile acids inhibit CCK release by a negative feedback mechanism which helps to relax and refill the gallbladder (24,171,202,248). Chemical sequestration of bile acids in dogs augmented the release of CCK and pancreatic enzyme secretion in response to amino acids and addition of taurocholate reversed this effect (89).…”

Section: Undermentioning

confidence: 99%

Regulation of Pancreatic Secretion

Liddle

The Pancreapedia: Exocrine Pancreas Knowledge Base

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Effects of duodenal perfusion with sodium taurocholate on biliary and pancreatic secretion in man

Cited by 22 publications

References 33 publications

Physicochemical Studies on Indocyanine Green: Molar Lineic Absorbance, pH Tolerance, Activation Energy and Rate of Decay in Various Solvents

Physicochemical Studies on Indocyanine Green: Molar Lineic Absorbance, pH Tolerance, Activation Energy and Rate of Decay in Various Solvents

Regulation of VLDL secretion in primary culture of rat hepatocytes: involvement of cAMP and cAMP‐dependent protein kinases

Regulation of Pancreatic Secretion

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