Glass samples with varying compositions was prepared by standard approach. The composition of the samples was (50-x) B2O3–25TeO2–25BaO-xDy2O3, with x ranging from 0 to 1.25 mol%. XRD profiles confirmed that the samples were amorphous, as there were no long-range lattice arrangements observed. The successful preparation of amorphous samples was further confirmed by the absence of sharp lines & peaks. The optical properties of the obtained samples were analyzed. That a decrease in Eopt values resulted in a higher value of the refractive index (n) for the glasses. However, when the concentration of Dy2O3 exceeded certain levels (0.75, 1, and 1.25 mol%), the refractive index (n) decreased due to an increase in Eopt values. Experimental measurements using a NaI(Tl) detector were conducted to determine the radiation shielding parameters (LAC and MAC), as well as the (HVL), (TVL), & (MFP) of the glasses against gamma rays emitted by 137Cs and 60Co isotopes at energies of 0.662, 1.173, and 1.333 MeV. Excellent agreement was observed when comparing the experimental results with theoretical calculations using Phy-x/PSD software program. This suggests that the fabricated glasses have great potential for various applications in the field of optics and can effectively shield against radiation.