Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

Lee, Seung-Chan; Seo, Ho-Chul; Lee, Jaewang; Jun, Jin Hyun; Choi, Kyoo Wan

doi:10.5653/cerm.2019.00514

Cited by 9 publications

(9 citation statements)

References 45 publications

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“…They found that peri-implantation development was not affected by individual culture under 5% oxygen, but under 20% oxygen, individual culture resulted in smaller outgrowths than embryos that had been cultured in groups, indicating they were less viable. We similarly showed that a dynamic oxygen concentration (decreasing from 5% to 2%) had beneficial effects on mouse pre-and peri-implantation development using an outgrowth model [33].…”

Section: History Of Research Using the Outgrowth Model Of Mammalian Bmentioning

confidence: 70%

Advantages of the outgrowth model for evaluating the implantation competence of blastocysts

Kim

Lee

Jun

2020

Clin Exp Reprod Med

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The implantation process is highly complex and difficult to mimic <i>in vitro</i>, and a reliable experimental model of implantation has yet to be established. Many researchers have used embryo transfer (ET) to assess implantation potential; however, ET with pseudopregnant mice requires expert surgical skills and numerous sacrificial animals. To overcome those economic and ethical problems, several researchers have tried to use outgrowth models to evaluate the implantation potential of embryos. Many previous studies, as well as our experiments, have found significant correlations between blastocyst outgrowth <i>in vitro</i> and implantation in utero by ET. This review proposes the blastocyst outgrowth model as a possible alternative to animal experimentation involving ET in utero. In particular, the outgrowth model might be a cost- and time-effective alternative method to ET for evaluating the effectiveness of culture conditions or treatments. An advanced outgrowth model and further culture of outgrowth embryos could provide a subtle research model of peri- and postimplantation development, excluding maternal effects, and thereby could facilitate progress in assisted reproductive technologies. Recently, we found that outgrowth embryos secreted extracellular vesicles containing specific microRNAs. The function of microRNAs from outgrowth embryos should be elucidated in further researches.

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Section: History Of Research Using the Outgrowth Model Of Mammalian Bmentioning

confidence: 70%

Advantages of the outgrowth model for evaluating the implantation competence of blastocysts

Kim

Lee

Jun

2020

Clin Exp Reprod Med

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“…Our study was approved by the ethics committee at the Eulji University Institutional Animal Care and Use Committee (EUIACUC 19-19, EUIACUC 20-12). The protocol for superovulation was applied according to our previous report [ 14 ]. Briefly, female ICR mice (aged 5-10 weeks) and male ICR mice (aged 10 weeks-3 months) were mated under controlled light-dark cycle (lights on at 8:00 AM; lights off at 8:00 PM).…”

Section: Methodsmentioning

confidence: 99%

“…In the DO group, zygotes were cultured under 5% O 2 conditions to the morula stage for 2 days and then cultured under 2% O 2 to the blastocyst stage for 2 days. Nitrogen and carbon dioxide concentrations were fine-tuned using a double-channel gas supply system, also used in our previous study [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Currently, there are no optimized culture systems that reflect the different oxygen con-centrations within the female reproductive tract. However, in vitro culture systems for preimplantation embryos that mimic oxygen concentrations in the in vivo female reproductive system have begun to emerge [13][14][15], despite lack of standards for the culture systems and conditions.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic Oxygen Conditions Promote the Translocation of HIF-1α to the Nucleus in Mouse Blastocysts

Choi

Kim

Yoon

et al. 2021

BioMed Research International

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Oxygen tension is one of the most critical factors for mammalian embryo development and its survival. The HIF protein is an essential transcription factor that activated under hypoxic conditions. In this study, we evaluated the effect of dynamic oxygen conditions on the expression of embryonic genes and translocation of hypoxia-inducible factor-1α (HIF-1α) in cultured mouse blastocysts. Two-pronuclear (2PN) zygotes harvested from ICR mice were subjected to either high oxygen (HO; 20%), low oxygen (LO; 5%), or dynamic oxygen (DO; 5% to 2%) conditions. In the DO group, PN zygotes were cultured in 5% O2 from days 1 to 3 and then in 2% O2 till day 5 after hCG injection. On day 5, the percentage of blastocysts in the cultured embryos from each group was estimated, and the embryos were also subjected to immunocytochemical and gene expression analysis. We found that the percentage of blastocysts was similar among the experimental groups; however, the percentage of hatching blastocysts in the DO and LO groups was significantly higher than that in the HO group. The total cell number of blastocysts in the DO group was significantly higher than that of both the HO and LO groups. Further, gene expression analysis revealed that the expression of genes related to the embryonic development was significantly higher in the DO group than that in the HO and LO groups. Interestingly, HIF-1α mRNA expression did not significantly differ; however, HIF-1α protein translocation from the cytoplasm to the nucleus was significantly higher in the DO group than in the HO and LO groups. Our study suggests that dynamic oxygen concentrations increase the developmental capacity in mouse preimplantation embryos through activation of the potent transcription factor HIF-1α.

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“…Studies have shown that reducing the oxygen concentration in the incubator was more conducive to embryo development in humans [ 16 ], cattle [ 17 ], mice [ 18 ], and swine [ 19 ]. In subsequent studies, 5% oxygen concentrations (hypoxic) were also used in vitro, resulting in a higher blastocyst rate and greater benefits before and after implantation compared with those of 20% oxygen concentrations (normoxia) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Transcriptome Analysis of mRNA Expression Patterns of Early Embryo Development in Goat under Hypoxic and Normoxic Conditions

Wan

Deng

et al. 2021

Biology

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It has been reported that hypoxic environments were more suitable for the in vitro development of mammalian embryos, but the underlying mechanisms were still unclear. In the present study, RNA-seq was performed to compare 8-cell-stage and blastocyst-stage goat embryos under hypoxic and normoxic conditions; zygotes were checked at 72 and 168 h to 8-cell stage (L8C) and blastocyst stage (LM) in hypoxic conditions and 8-cell stage (H8C) and blastocyst stage (HM) in normoxic conditions. In the H8C and L8C groups, 399 DEGs were identified, including 348 up- and 51 down-regulated DEGs. In the HM and LM groups, 1710 DEGs were identified, including 1516 up- and 194 down-regulated DEGs. The expression levels of zygotic genes, transcription factors, and maternal genes, such as WEE2, GDF9, HSP70.1, BTG4, and UBE2S showed significant changes. Functional enrichment analysis indicated that these DEGs were mainly related to biological processes and function regulation. In addition, combined with the pathway–gene interaction network and protein–protein interaction network, twenty-two of the hub genes were identified and they are mainly involved in energy metabolism, immune stress response, cell cycle, receptor binding, and signal transduction pathways. The present study provides comprehensive insights into the effects of oxidative stress on early embryo development in goats.

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Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

Cited by 9 publications

References 45 publications

Advantages of the outgrowth model for evaluating the implantation competence of blastocysts

Advantages of the outgrowth model for evaluating the implantation competence of blastocysts

Dynamic Oxygen Conditions Promote the Translocation of HIF-1α to the Nucleus in Mouse Blastocysts

Comprehensive Transcriptome Analysis of mRNA Expression Patterns of Early Embryo Development in Goat under Hypoxic and Normoxic Conditions

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