Effects of dynamic radial tensile stress on fibrocartilage differentiation of bone marrow mesenchymal stem cells

Su, Xuelian; Wang, Jizeng; Kang, Hong; Bao, Guangjie; Liu, Lin

doi:10.1186/s12938-020-0751-1

Cited by 9 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4) The restoration of the early mechanical properties could also better promote the regeneration of fibrocartilage. Some researchers have proposed that a good mechanical environment could better promote the regeneration of fibrocartilage ( 52 , 53 ). On the basis of our in vivo experimental results, the mechanical properties in the s-Cu/Zn-gelatin groups, such as earlier tensile force and greater stiffness, are better than those of the other groups and provide a better mechanical environment to promote the regeneration of fibrocartilage.…”

Section: Discussionmentioning

confidence: 99%

Gradient bimetallic ion–based hydrogels for tissue microstructure reconstruction of tendon-to-bone insertion

et al. 2021

View full text Add to dashboard Cite

Although gradients play an essential role in guiding the function of tissues, achieving synchronous regeneration of gradient tissue injuries remains a challenge. Here, a gradient bimetallic (Cu and Zn) ion–based hydrogel was first constructed via the one-step coordinative crosslinking of sulfhydryl groups with copper and zinc ions for the microstructure reconstruction of the tendon-to-bone insertion. In this bimetallic hydrogel system, zinc and copper ions could not only act as crosslinkers but also provide strong antibacterial effects and induce regenerative capacity in vitro. The capability of hydrogels in simultaneously promoting tenogenesis and osteogenesis was further verified in a rat rotator cuff tear model. It was found that the Cu/Zn gradient layer could induce considerable collagen and fibrocartilage arrangement and ingrowth at the tendon-to-bone interface. Overall, the gradient bimetallic ion–based hydrogel ensures accessibility and provides opportunities to regenerate inhomogeneous tissue with physiological complexity or interface tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Gradient bimetallic ion–based hydrogels for tissue microstructure reconstruction of tendon-to-bone insertion

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Small nucleolar RNA host gene (SNHG)14 is a newly discovered long non-coding RNA that could induce osteogenic differentiation of hMSCs in vitro by targeting miR-2861 [ 81 ]. Although it would be much easier to use only hMSCs (one cell source) for all three zones, the ligamentous [ 82 ] and fibrocartilaginous [ 83 , 84 , 85 ] differentiation of hMSCs is very controversially discussed. In contrast to another study, where the colonization of a decellularized scaffold was performed with a zonal application of growth factors [ 53 ], no supplements except for ascorbic acid in the growth medium to stimulate collagen synthesis were used in this study.…”

Section: Discussionmentioning

confidence: 99%

Co-Culture of Mesenchymal Stem Cells and Ligamentocytes on Triphasic Embroidered Poly(L-lactide-co-ε-caprolactone) and Polylactic Acid Scaffolds for Anterior Cruciate Ligament Enthesis Tissue Engineering

Gögele

Vogt

Hahn

et al. 2023

IJMS

View full text Add to dashboard Cite

Successful anterior cruciate ligament (ACL) reconstructions strive for a firm bone-ligament integration. With the aim to establish an enthesis-like construct, embroidered functionalized scaffolds were colonized with spheroids of osteogenically differentiated human mesenchymal stem cells (hMSCs) and lapine (l) ACL fibroblasts in this study. These triphasic poly(L-lactide-co-ε-caprolactone) and polylactic acid (P(LA-CL)/PLA) scaffolds with a bone-, a fibrocartilage transition- and a ligament zone were colonized with spheroids directly after assembly (DC) or with 14-day pre-cultured lACL fibroblast and 14-day osteogenically differentiated hMSCs spheroids (=longer pre-cultivation, LC). The scaffolds with co-cultures were cultured for 14 days. Cell vitality, DNA and sulfated glycosaminoglycan (sGAG) contents were determined. The relative gene expressions of collagen types I and X, Mohawk, Tenascin C and runt-related protein (RUNX) 2 were analyzed. Compared to the lACL spheroids, those with hMSCs adhered more rapidly. Vimentin and collagen type I immunoreactivity were mainly detected in the hMSCs colonizing the bone zone. The DNA content was higher in the DC than in LC whereas the sGAG content was higher in LC. The gene expression of ECM components and transcription factors depended on cell type and pre-culturing condition. Zonal colonization of triphasic scaffolds using spheroids is possible, offering a novel approach for enthesis tissue engineering.

show abstract

“…However, a higher stretch stimulus (15%) simultaneously promoted the synthesis of α-smooth muscle actin. Therefore, 10% radial stretch stimulation is the optimal intensity to induce differentiation of BMSCs into fibrochondrocytes [ 69 ].…”

Section: Mechanical Microenvironment For Stem Cell Growthmentioning

confidence: 99%

How the mechanical microenvironment of stem cell growth affects their differentiation: a review

Zhang

Wang

2022

Stem Cell Res Ther

View full text Add to dashboard Cite

Stem cell differentiation is of great interest in medical research; however, specifically and effectively regulating stem cell differentiation is still a challenge. In addition to chemical factors, physical signals are an important component of the stem cell ecotone. The mechanical microenvironment of stem cells has a huge role in stem cell differentiation. Herein, we describe the knowledge accumulated to date on the mechanical environment in which stem cells exist, which consists of various factors, including the extracellular matrix and topology, substrate stiffness, shear stress, hydrostatic pressure, tension, and microgravity. We then detail the currently known signalling pathways that stem cells use to perceive the mechanical environment, including those involving nuclear factor-kB, the nicotinic acetylcholine receptor, the piezoelectric mechanosensitive ion channel, and hypoxia-inducible factor 1α. Using this information in clinical settings to treat diseases is the goal of this research, and we describe the progress that has been made. In this review, we examined the effects of mechanical factors in the stem cell growth microenvironment on stem cell differentiation, how mechanical signals are transmitted to and function within the cell, and the influence of mechanical factors on the use of stem cells in clinical applications.

show abstract

Effects of dynamic radial tensile stress on fibrocartilage differentiation of bone marrow mesenchymal stem cells

Cited by 9 publications

References 29 publications

Gradient bimetallic ion–based hydrogels for tissue microstructure reconstruction of tendon-to-bone insertion

Gradient bimetallic ion–based hydrogels for tissue microstructure reconstruction of tendon-to-bone insertion

Co-Culture of Mesenchymal Stem Cells and Ligamentocytes on Triphasic Embroidered Poly(L-lactide-co-ε-caprolactone) and Polylactic Acid Scaffolds for Anterior Cruciate Ligament Enthesis Tissue Engineering

How the mechanical microenvironment of stem cell growth affects their differentiation: a review

Contact Info

Product

Resources

About