Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs

Kim, Seong‐Ki; Cho, Myung-Woo; Kim, Junsu; Jang, Ki Beom; Kim, Sheen-A; Mun, Daye; Kim, Byeong-Hyeon; Kim, Young-Hwa; Park, Jun-Cheol; Choe, Jae Gol; Song, Minho

doi:10.11625/kjoa.2018.26.1.141

Cited by 5 publications

(10 citation statements)

References 11 publications

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“…2). The improved nutrient digestibility in this study was similar to results reported by previous studies [11,27,28]. Addition of exogenous enzymes in non-ruminant diets has been using for several benefits including, minimization of anti-nutritional effects and maximization of nutrient utilization, to improve nutrient utilization and growth performance of non-ruminants [29] because the digestive system and the secretion and activity of digestive enzymes of young non-ruminants are immature and insufficient Sangwoo Park, et al Each value is the mean of 5 replicates (5 pigs/pen).…”

Section: Resultssupporting

confidence: 92%

“…However, no difference was found on growth performance of weaned pigs during overall experimental period between PC and PR ( Table 2). These results are similar to the results from previous studies for weaned pigs [10,11,21] and for growing pigs [14,25]. The reason for these observations may be related to the improved nutrient utilization efficiency by addition of PR [25].…”

Section: Resultssupporting

confidence: 91%

“…After anesthesia, pigs were euthanized by CO 2 gas [19]. Ileal digesta samples were collected from distal ileum before the ileocecal junction [11]. The collected ileal digesta samples were stored at -20℃ until analysis.…”

Section: Competing Interestsmentioning

confidence: 99%

“…The collected ileal digesta samples were stored at -20℃ until analysis. Sections of ileum of 3 cm length were collected, washed gently with distilled water, and fixed in 10% neutral buffered formalin for histological analysis [20,21].…”

Section: Competing Interestsmentioning

confidence: 99%

“…Dietary protease (PR) is an exogenous enzyme able to degrade proteins and has being widely used in swine diets as a part of multi-enzyme products [10]. Several studies have reported supplementation of enzyme cocktails with protease in swine diets enhanced protein digestion and growth performacnce of weaning, growing, and finishing pigs [11][12][13]. Recent studies also reported addition of PR alone in swine diets impoved gut development and health of weaned pigs by breaking down protein-bound complexes with other ANFs and affected positively nutrient digestibility and growth performace of pigs [9,[11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs

Park¹,

Lee²,

Yang³

et al. 2020

J Anim Sci Technol

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This study was conducted to evaluate effects of dietary protease (PR) on growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. A total of 75 weaned pigs [7.06 ± 0.18 kg of average body weight (BW); 28 day old] were randomly allotted to 3 dietary treatments in a randomized complete block design (blocks = BW and sex): a diet based on corn and soybean meal to meet the requirement of crude protein (CP) as a positive control (PC; CP = 24.49%), a low protein diet as a negative control (NC; CP = 22.51%), and NC + 0.02% PR. The PR used in this study was a commercial product containing 75,000 protease units/g derived from Nocardiopsis prasina produced in Bacillus licheniformis. Pigs were fed the dietary treatments for 6 weeks and the diets containing 0.2% chromic oxide for the last week of this study. Blood, feces, ileal digesta, and ileum samples were collected from randomly selected two pigs in each pen on respective time points. Measurements were growth performance, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter (DM), CP, and energy, frequency of diarrhea, packed cell volume (PCV), and ileal morphology of weaned pigs. Pigs fed PC and PR had higher (p < 0.05) final BW, average daily gain (ADG), and gain to feed ratio (G:F) during overall experimental period than those fed NC. Pigs fed PC and PR had higher (p < 0.05) AID or ATTD of DM, CP, or energy than those fed NC. Moreover, pigs fed PR had higher ratio between villus height and crypt depth (p < 0.05) and number of goblet cells (p < 0.05) than those fed NC. Addition of PR decreased (p < 0.05) frequency of diarrhea for the first two weeks after weaning compared with PC and NC. In addition, pigs fed PR had lower (p < 0.05) PCV on d 14 after weaning than those fed PC and NC. In conclusion, addition of PR in nursery diets with a low protein level significantly improved growth performance, nutrient digestibility, and intestinal morphology of weaned pigs.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Section: Competing Interestsmentioning

confidence: 99%

Section: Competing Interestsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs

Park¹,

Lee²,

Yang³

et al. 2020

J Anim Sci Technol

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The Effects of Phytase and Non-Starch Polysaccharide-Hydrolyzing Enzymes on Trace Element Deposition, Intestinal Morphology, and Cecal Microbiota of Growing–Finishing Pigs

Liu

et al. 2023

Animals

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This study investigated the effects of supplementing phytase and non-starch polysaccharide-degrading enzymes (NSPases) to corn–soybean meal-based diet on the growth performance, trace element deposition, and intestinal health of growing–finishing pigs. Fifty pigs were randomly assigned into the control (basal diet), phytase (basal diet + 100 g/t of phytase), β-mannanase (basal diet + 40 g/t of β-mannanase), β-glucanase (basal diet + 100 g/t of β-glucanase), and xylanase (basal diet + 100 g/t of xylanase) groups. The results show that the supplementation of phytase and NSPases had no impacts (p > 0.05) on the growth performance of pigs. Compared with the control group, pigs fed with xylanase had higher (p < 0.05) Zn concentrations in the ileum and muscle and those fed with phytase had higher (p < 0.05) Zn concentrations in the ileum. Phytase and xylanase supplementation decreased (p < 0.05) fecal Zn concentrations in pigs compared with the control group (p < 0.05). In addition, phytase, β-mannanase, β-glucanase, and xylanase supplementation up-regulated (p < 0.05) the FPN1 expression, whereas xylanase up-regulated (p < 0.05) the Znt1 expression in the duodenum of pigs compared with the control group. Moreover, phytase, β-glucanase, and xylanase supplementation up-regulated (p < 0.05) the jejunal Znt1 expression compared with the control group. The intestinal morphology results show that the phytase, β-mannanase, and xylanase groups had increased villus heights (VHs), an increased villus height–crypt depth ratio (VH:CD), and decreased crypt depths (CDs) in the duodenum, whereas phytase, β-mannanase, β-glucanase, and xylanase groups had decreased VH and VH:CD, and increased CD in the jejunum compared with the control group (p < 0.05). Pigs fed with exogenous enzymes had decreased bacterial diversity in the cecum. The dietary supplementation of NSPases increased the relative abundance of Firmicutes and decreased spirochaetes (p < 0.05). Compared with the control group, dietary NSPase treatment decreased (p < 0.05) the opportunistic pathogens, such as Treponema_2 and Eubacterium_ruminantium. Moreover, the relative abundances of Lachnospiraceae_XPB1014 and Lachnospiraceae were enriched in the β-glucanase and β-mannanase groups (p < 0.05), respectively. In conclusion, phytase and xylanase supplementation may promote zinc deposition in pigs. Additionally, the supplementation of NSPases may improve the gut health of pigs by modulating the intestinal morphology and microbiota.

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Effects of dietary protease on immune responses of weaned pigs

Lee¹,

Kang²,

Park³

et al. 2020

J Anim Sci Technol

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This experiment was conducted to investigate effects of dietary protease on immune responses of weaned pigs. Weaned pigs (n = 75; 7.06 ± 0.18 kg BW; 28 d old) were randomly assigned to 3 treatments (5 pigs/pen; 5 pens/treatment). Dietary treatments were positive control, a diet with required protein level (PC), negative control, a diet with lower protein level than PC (NC), and NC + 0.02% dietary protease (PRO). The dietary protease used in this experiment was a commercial product containing 75,000 protease units/g derived from Nocardi opsis prasina produced in Bacillus licheniformis. The dietary treatments did not contain any ingredients or additives that may provide antibacterial or physiological effects. Pigs were fed respective dietary treatments for 6 weeks. Blood was collected from randomly selected 2 pigs in each pen on d 1, 3, 7, and 14 after weaning. Measurements were number of white blood cells (WBC), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1), and C-reactive protein (CRP). Pigs fed PRO had lower WBC on d 7 (14.84 vs 20.42 × 10 3 / μL; p < 0.05) and TNF-α on d 7 (618 vs 889 pg/mL; p = 0.085) and 14 (437 vs 576 pg/mL; p = 0.069) than those fed NC, but there were no differences on WBC and TNF-α between PC and PRO. Pigs fed PRO had lower TGF-β1 on d 3 (630 vs. 1,588 and 1,396 pg/mL; p < 0.05) than those fed PC and NC. However, no differences were found on CRP among dietary treatments. In conclusion, addition of dietary protease reduced inflammatory immune responses of weaned pigs.

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Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs

Cited by 5 publications

References 11 publications

Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs

Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs

The Effects of Phytase and Non-Starch Polysaccharide-Hydrolyzing Enzymes on Trace Element Deposition, Intestinal Morphology, and Cecal Microbiota of Growing–Finishing Pigs

Effects of dietary protease on immune responses of weaned pigs

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