Effects of Educated Monocytes with Xenogeneic Mesenchymal Stem Cell–Derived Conditioned Medium in a Mouse Model of Chronic Asthma

Galeh, Hadi Esmaili Gourvarchin; Froushani, Seyyed Meysam Abtahi; Ahangaran, Nahideh Afzale; Hadai, Siamak Naji

doi:10.1080/08820139.2018.1458108

Cited by 13 publications

(4 citation statements)

References 45 publications

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“…Based on the pathogenicity of disease, neutrophils are the essential immune cells migrating into the colonic tissue after luminal instilment of acetic acid and induce inflammatory damages by the formation of oxygen and nitrogen species [19] potentially be an approach for cell-based treatments of mice model of asthma [9]. Accordingly, it was formerly documented that MSCs could increase the anti-oxidant capability of small bowel tissue after intestinal ischemia-reperfusion damage [22].…”

Section: Discussionmentioning

confidence: 99%

“…The supernatants were extracted and centrifuged for 10 minutes at 300 g to remove cell remnants. Afterward, a mixture was prepared from the harvested supernatants from every BALB/c mice, passed through a 0.2-μm filter orderly, and then kept frozen at -80ºC before tests [9].…”

Section: Isolation Of Mscs and Supernatant Preparationmentioning

confidence: 99%

“…Following that, attached monocytes were cultured in DMEM enriched with 10% FBS and 50% bone marrow-MSCs-Sp or DMEM enriched with 10% FBS and 50% bone marrow-MSCs-Sp (negative control media). After 24 hours, the cells were harvested, rinsed three times, and labeled as untreated or treated for in vivo assays [9].…”

Section: Monocyte Isolation and Treatmentmentioning

confidence: 99%

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Evaluation of the Anti-Oxidative Effects of Monocyte Cells Treated with Bone Marrow Mesenchymal Stem Cells Supernatant on Experimental Colitis in BALB/c Mice

Ghaleh

Kondori

Razaviyan

et al. 2021

GMJ

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Background: Alternate activation of monocytes could induce anti-inflammatory impacts. This study aimed to investigate whether monocyte cells treated with bone marrow mesenchymal stem cells supernatant (MSC-Sp) could improve anti-inflammatory responses as a cell transfer therapy for colitis. Materials and Methods: The induction of experimental colitis was done by acetic acid in four groups of male BALB/c mice, including the control colitis, treated-monocytes, non-treated-monocytes, and mesalazine groups. Following MSCs culture, the supernatant was harvested, and then 50% conditioned media, or negative control media was added to the monocytes for 24 h. After ten days, peritoneal injection of treated or non-treated-monocytes (105 cells/100µL) was performed in animals' relevant groups of colitis. Ten days later, the oxidative stress profile and histopathological evaluation of colon tissue were assessed. Results: Treated monocytes showed a significant improvement in the oxidative stress profile, namely myeloperoxidase (0.126±0.008), nitric oxide (0.153±0.01), and malondialdehyde (0.148±0.014) compared to the control colitis group (P<0.05). Also, histopathological results revealed that the rate of damage in the treated-monocytes group was less than in normal mice. Conclusion: Our study indicated that the treated monocytes had anti-oxidative potential in colitis mice and were usable as a complementary therapy. [GMJ.2021;10:e2131]

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Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Mscs and Supernatant Preparationmentioning

confidence: 99%

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Evaluation of the Anti-Oxidative Effects of Monocyte Cells Treated with Bone Marrow Mesenchymal Stem Cells Supernatant on Experimental Colitis in BALB/c Mice

Ghaleh

Kondori

Razaviyan

et al. 2021

GMJ

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“…Then cell suspensions (2 × 10 6 cells/mL) underwent incubation in plates with 24 wells and were pulsated by PHA solution of 50 μL (1 mg/mL). The culture supernatants were gathered after 72 h. IL-10 and IL-17 cytokines were assayed by ELISA test according to the instructions of the provider of the kits [17].…”

Section: Measuring Cytokinesmentioning

confidence: 99%

Pistacia atlantica gum aqueous extract modulates humoral and cellular immune responses in BALB/c mice

Ghaleh¹,

Gandabeh²,

Motlagh³

et al. 2020

Turk J Vet Anim Sci

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Natural herbal products contain different compounds that can modulate immune and inflammatory diseases. This investigation aimed to examine the influence of Pistacia atlantica aqueous extract on cellular and humoral immunity in mice following the exposure to sheep red blood cells (SRBCs). For this purpose, male BALB/c mice (n = 40) were randomly assigned into 4 equivalent groups. The animals received 1 × 10 9 SRBCs in complete Freund's adjuvant (CFA) intraperitoneally 3 times with two-week intervals. Five days after the final injection, blood samples were taken from the animals. Additionally, 1 × 10 9 SRBCs were also injected into the mice's left hind footpad 48 h before the blood collection. Three groups of mice received various oral doses of the extract (100, 200, and 400 mg/kg) for 52 consecutive days after the study onset. Measurements were performed on the levels of humoral and cellular immune responses, splenocytes' ability to uptake neutral red, respiratory burst, and cell viability. The mice receiving Pistacia atlantica in all concentrations displayed significantly low delayed type hypersensitivity (DTH) reactions in a dose-dependent manner compared to the control animals. The treated mice also presented a dose-dependent, significantly lower average anti-SRBC titer than that in the control animals. IL-17 and IL-10 cytokine levels respectively decreased and increased significantly in the mice that received Pistacia atlantica compared to the control group. Splenocyte proliferation and respiratory burst declined significantly in mice treated with Pistacia atlantica as opposed to the normal control group but phagocytosis ability was significantly decreased. The immunomodulatory impacts of Pistacia atlantica might be partially caused by immunity diversion from proinflammatory cytokine IL-17 into antiinflammatory cytokine IL-10.

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Mesenchymal stem cells in allergic diseases: Current status

Xie

et al. 2020

Allergology International

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Effects of Educated Monocytes with Xenogeneic Mesenchymal Stem Cell–Derived Conditioned Medium in a Mouse Model of Chronic Asthma

Abstract: The educated monocytes with MCM may be as a promising strategy for cell-based therapies of asthma.

Cited by 13 publications

References 45 publications

Evaluation of the Anti-Oxidative Effects of Monocyte Cells Treated with Bone Marrow Mesenchymal Stem Cells Supernatant on Experimental Colitis in BALB/c Mice

Evaluation of the Anti-Oxidative Effects of Monocyte Cells Treated with Bone Marrow Mesenchymal Stem Cells Supernatant on Experimental Colitis in BALB/c Mice

Pistacia atlantica gum aqueous extract modulates humoral and cellular immune responses in BALB/c mice

Mesenchymal stem cells in allergic diseases: Current status

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