Effects of electrical pulses on the distribution of radioactive phosphate in cerebral tissues

Incorporation of [32P]orthophosphate into brain-slice phospholipids and their precursors. Effects of electrical stimulation

“…Electrical stimulation caused a fall in the relative specific radioactivity of inorganic phosphate in the slice. Under different conditions, a similar effect of electrical stimulation was noticed by Heald (1956); he attributed it to faster equilibration of phosphorus in the trichloroacetic acidinsoluble fraction with that in the acid-soluble phosphates. The relative specific radioactivity of the y-phosphate group of ATP was decreased slightly but not significantly by electrical stimulation.…”

The composition of isolated cerebral tissues: ascorbic acid and cozymase

“…Prompt fixation after applying electrical pulses revealed no change in cozymase level. These experiments ( Table 1, B) employed conditions under which glycolysis and other aspects of carbohydrate metabolism had been shown to undergo rapid change (Heald, 1956;McIlwain & Tresize, 1956). Time (min.)…”

The Effects of Potassium Ion Concentration on Phosphate Metabolism in Cerebral Slices

“…After 5 sec the slice was removed, rapidly drained on a clock glass and transferred to 4.0 ml of trichloroacetic acid (10% w/v) a t 0". It was shown previously that this procedure did not significantly alter the levels of inorganic phosphate and phosphocreatine in the slices (HEALD, 1956) and resulted in only a small contamination of tissue inorganic phosphate with that of the medium. The slices from each hemisphere were combined, ground in a small centrifuge tube with a loosely fitting pestle and centrifuged in the high-speed attachment of the M.S.E.…”