Effects of electrically-induced constant tension on giant unilamellar vesicles using irreversible electroporation

Karal, Mohammad Abu Sayem; Ahamed, Md. Kabir; Rahman, M. Mostafizur; Ahmed, Marzuk; Shakil, Mostofa; Siddique-e-Rabbani, Khondkar

doi:10.1007/s00249-019-01398-9

Cited by 29 publications

(24 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, an attempt to evaluate a stimulus-dependent response of giant unilamellar lipid vesicles (GUV) using a series of electric field pulses of micro- to millisecond duration, has been demonstrated to be successful in electrodeformation, electrofusion and electroporation in the membranes of these vesicles ( Karal et al, 2019 ). Authors proved that the results of pore formation at different electrical-induced membrane tensions are in agreement with those reported for mechanical-induced ones.…”

Section: Introductionmentioning

confidence: 99%

Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments

Caramazza

Nardoni

Angelis

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments

Caramazza

Nardoni

Angelis

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

“…Therefore, the spherical structure of GUV was permanently disappeared due to the rupture of vesicle. In our several previous papers, we explained the rupture of GUVs in such a way that at first a nanopore is formed in the membranes whose radius rapidly increases to infinity, leading to complete rupture of GUVs [ 38 , 46 , 56 ]. The time of pore formation is defined as the time when the vesicles started to rupture.…”

Section: Results and Analysismentioning

confidence: 99%

“…Here, we describe the experimental technique on the electric field-induced rupture of vesicles in the presence of different P. A MOSFET based IRE circuit was used for generating direct current (DC) pulses of frequency 1.1 kHz and pulse width 200 μs in which electric signal was controlled with a microcontroller. The detail of the method is described in our reported papers [38,46]. The gold coated electrode was used for applying the electric field on GUVs.…”

Section: Methods Of Applying Constant Electric Tension On Guvs Under Osmotic Pressurementioning

confidence: 99%

“…DOPG/DOPC/chol (46/39/15)-GUVs (46/39/15 indicates molar ratio) and DOPG/DOPC (40/60)-GUVs were prepared in a buffer (10 mM PIPES, 150 mM NaCl, pH 7.0, 1 mM EGTA) containing 100 mM sucrose using the well-known natural swelling method [ 37 , 38 ]. The surface charge density of DOPG/DOPC (40/60)-GUVs is = – 0.10 Cm -2 where, X (= 0.40) is the DOPG molar fraction in membranes, e is the electronic charge and a DOPG (= 72.5 Å 2 /molecule) is the cross sectional area of DOPG [ 39 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles

et al. 2021

Self Cite

View full text Add to dashboard Cite

Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC, with specific molar ratios. We determined the rate constant (kp) of rupture of DOPG/DOPC/chol (46/39/15)-GUVs and DOPG/DOPC (40/60)-GUVs induced by constant electric tension (σc) under different Π. The σc dependent kp values were fitted with a theoretical equation, and the corresponding membrane tension (σoseq) at swelling equilibrium under Π was estimated. The estimated membrane tension agreed well with the theoretical calculation within the experimental error. Interestingly, the values of σoseq were almost same for both types of synthesized GUVs under same osmotic pressure. We also examined the sucrose leakage, due to large osmotic pressure-induced pore formation, from the inside of DOPG/DOPC/chol(46/39/15)-GUVs. The estimated membrane tension due to large Π at which sucrose leaked out was very similar to the electric tension at which GUVs were ruptured without Π. We explained the σc and Π induced pore formation in the lipid membranes of GUVs.

show abstract

“…5C-F). Additionally membrane tension was important in membrane dynamics triggered by electric fields, 36 and a membrane relaxation preceded liposome buddings (Supplementary Movies S1-S3 and S9). These implied that 400 ls, 1.248 kV/cm PEF imposed electrical stress without electroporation on membranes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Pulsed Electric Fields Promote Liposome Buddings

Urabe

Ogata

et al. 2021

Bioelectricity

View full text Add to dashboard Cite

Background: Liposomes have been a useful tool to analyze membrane behavior. Various studies have attempted to induce biological activities, for example, buddings, divisions, and endocytosis, on liposomes, focusing on lipid rafts that move along electric fields. Materials and Methods: Liposomes consisting of soybean lecithin, phosphatidylcholine, and cholesterol were prepared, with inner and outer liquid conductivities of 0.595 and 1.564 S/m, respectively. Results: We tried to induce buddings by pulsed electric fields (PEFs) on liposomes. Results demonstrated that 1.248 kV/cm, 400 ls PEF promoted postpulse liposome buddings, which were preceded by a membrane relaxation. Although a transient thick area (a lipid raft-like area) on the membrane just after PEF application preceded buddings, it was not the sufficient factor for buddings. Conclusion: We established a brief model as follows: 1.248 kV/cm, 400 ls PEF induced the lipid membrane relaxation without electroporation to trigger buddings. The current results could be a new frontier in bioelectrics.

show abstract

Effects of electrically-induced constant tension on giant unilamellar vesicles using irreversible electroporation

Cited by 29 publications

References 52 publications

Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments

Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments

Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles

Pulsed Electric Fields Promote Liposome Buddings

Contact Info

Product

Resources

About