Effects of Electromagnetic Fields on Cells: Physiological and Therapeutical Approaches and Molecular Mechanisms of Interaction

Funk, Richard; Monsees, Thomas K.

doi:10.1159/000093061

Cited by 145 publications

(107 citation statements)

References 245 publications

(142 reference statements)

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“…Therefore, coupling of an electromagnetic field with the live cell can occur via either field interaction with charged molecules and proteins in the cell membrane that alters the flow of ions through the ion channels or rearranges the distribution of the membrane receptors, or via direct field penetration inside the cell and interaction with charged entities in the cytoplasm [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Modulation of cell function by electric field: a high-resolution analysis

Taghian

Narmoneva

Kogan

2015

J. R. Soc. Interface.

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Regulation of cell function by a non-thermal, physiological-level electromagnetic field has potential for vascular tissue healing therapies and advancing hybrid bioelectronic technology. We have recently demonstrated that a physiological electric field (EF) applied wirelessly can regulate intracellular signalling and cell function in a frequency-dependent manner. However, the mechanism for such regulation is not well understood. Here, we present a systematic numerical study of a cell-field interaction following cell exposure to the external EF. We use a realistic experimental environment that also recapitulates the absence of a direct electric contact between the field-sourcing electrodes and the cells or the culture medium. We identify characteristic regimes and present their classification with respect to frequency, location, and the electrical properties of the model components. The results show a striking difference in the frequency dependence of EF penetration and cell response between cells suspended in an electrolyte and cells attached to a substrate. The EF structure in the cell is strongly inhomogeneous and is sensitive to the physical properties of the cell and its environment. These findings provide insight into the mechanisms for frequency-dependent cell responses to EF that regulate cell function, which may have important implications for EF-based therapies and biotechnology development.

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Section: Introductionmentioning

confidence: 99%

Modulation of cell function by electric field: a high-resolution analysis

Taghian

Narmoneva

Kogan

2015

J. R. Soc. Interface.

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“…According to Miyakoshi, 2006, electromagnetic fields induce DNA damages [13]. Funk, in 2006 also shows electromagnetic fields could reduce cAMP in bone cell culture up to 80% which is one of the most important inhibitory cell proliferation agents [14]. Since each cell have some electric parameter which cause ion charge in cell membrane and cancerous cell had a depolarize ruined membrane with permeability to water and Na+ which lead to anaerobic respiration, hypoxia stress and releasing angiogenic factors, angiogenesis and metastasis [15].…”

Section: Discussionmentioning

confidence: 99%

Role of Low Frequency Bio Electromagnetic Field on Angiogenesis Biomarkers in Human Breast Tumor Cell Line

Baharara¹,

Mousavi²

2014

International Journal of Cellular and Molecular Biotechnology

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Many biological processes are affected by electromagnetic fields. Effect of electromagnetic field on proliferation, cell division, wound healing and gene expression were examined. Angiogenesis, new blood vessel formation, which is required for embryonic development and many physiological events, plays a crucial role in the pathobiology of tumors and researches show angiogenesis inhibition could be an effective therapeutic strategy for patients with cancer. In this research, effects of low-frequency electromagnetic field on VEGF-A, VEGFR-2 and MMP gene expression as potent biomarkers of angiogenesis was investigated in human breast cancer cells. MCF-7 cells were grown in RPMI 1640 medium supplemented with 10% FBS and incubated at 37˚C with 5% CO2. After 48h all flasks exposed with electromagnetic field (50Hz, 0.004 T) then total RNA was extracted and cDNA was synthesized using specific primer. Finally synthesized products were analyzed by Real Time PCR to determine expression level of VEGF-A, VEGFR-2 and MMP. Data was analyzed by SPSS software ANOVA and Tukey. Data analysis shows inhibitory effect of low-frequency bio electromagnetic field with 50Hz, 400 gauss intensity in MCF-7 cell line compared with control group. Although all genes did not show same reduction but gene expression in all cases affected by low-frequency electromagnetic field. Significant inhibitory effect on gene expression was for VEGFR-2 with 25% decrease P<0.001. Matrix Metalloproteinase gene level decreased 8% (P˂0.05) while it was noticeable for VEGF-A at P˃0.05. Results indicate decreases in the expression of biomarkers of angiogenesis in the treated samples with lowfrequency electromagnetic field with 400 gauss intensity in MCF-7 cell line compared with control group which may be promising prevention for angiogenesis related diseases.

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“…The results of these studies are in accordance with the present data that ELF-MF may act as a stressful factor to alter the cell structure and function in digestive system. Although the mechanism of action of ELF-MF with biological structures is up to now largely unknown, it has been proposed that the cell membrane must be the main target for interaction [34].…”

Section: Discussionmentioning

confidence: 99%