“…Mechanical testing of regenerated areas is also used as an evaluation strategy (6 out of 66). To quantify the amount of repaired bone from histological and/or immunohistochemical analysis, either image analysis software is used (mostly ImageJ, 92,125,127,132,142,143,150,161,167–169 Image‐Pro Plus 119,122,157,170–172 or i‐solution 120,133,136 ), or a scoring system 173 is applied. Approximately 50% of studies do not show quantification of the histological and immunohistochemical images leading to potential biased and subjective analyses.…”