Effects of electrospun fibers containing ascorbic acid on oxidative stress reduction for cardiac tissue engineering

Liver disease cases are rapidly expanding worldwide, and transplantation remains the only effective cure for end-stage disease. There is an increasing demand for developing potential drug treatments, and regenerative therapies using in-vitro culture platforms. Human decellularized extracellular matrix (dECM) is an appealing alternative to conventional animal tissues as it contains human-specific proteins and can serve as scaffolding materials. Herein we exploit this with human donor tissue from discarded liver which was not suitable for transplant using a synergistic approach to combining biological and topographical cues in electrospun materials as an in-vitro culture platform. To realise this, we developed a methodology for incorporating human liver dECM into electrospun polycaprolactone (PCL) fibres with surface nanotopographies (230–580 nm). The hybrid scaffolds were fabricated using varying concentrations of dECM; their morphology, mechanical properties, hydrophilicity and stability were analysed. The scaffolds were validated using HepG2 and primary mouse hepatocytes, with subsequent results indicating that the modified scaffolds-maintained cell growth and influenced cell attachment, proliferation and hepatic-related gene expression. This work demonstrates a novel approach to harvesting the potential from decellularized human tissues in the form of innovative in-vitro culture platforms for liver.

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Combining Human Liver ECM with Topographically Featured Electrospun Scaffolds for Hybrid Hepatic Models

Gao,

Gadd,

Heim

et al. 2024

Preprint

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Liver disease cases are rapidly expanding worldwide, and transplantation remains the only effective cure for end-stage disease. There is an increasing demand for developing potential drug treatments, and regenerative therapies using in-vitro culture platforms. Human decellularized extracellular matrix (dECM) is an appealing alternative to conventional animal tissues as it contains human-specific proteins and can serve as scaffolding materials. Herein we exploit this with human donor tissue from discarded liver which was not suitable for transplant using a synergistic approach to combining biological and topographical cues in electrospun materials as an in-vitro culture platform. To realise this, we developed a methodology for incorporating human liver dECM into electrospun polycaprolactone (PCL) fibres with surface nanotopographies (230-580 nm). The hybrid scaffolds were fabricated using varying concentrations of dECM; their morphology, mechanical properties, hydrophilicity and stability were analysed. The scaffolds were validated using HepG2 and primary mouse hepatocytes, with subsequent results indicating that the modified scaffolds-maintained cell growth and influenced cell attachment, proliferation and hepatic-related gene expression. This work demonstrates a novel approach to harvesting the potential from decellurised human tissues in the form of innovative in-vitro culture platforms for liver.

show abstract

Effects of electrospun fibers containing ascorbic acid on oxidative stress reduction for cardiac tissue engineering

Cited by 5 publications

References 109 publications

Comparative analysis of scaffold fibre diameter-modulated responses on immortalized and primary thyroid cells: Implications for thyroid regeneration

Comparative analysis of scaffold fibre diameter-modulated responses on immortalized and primary thyroid cells: Implications for thyroid regeneration

Combining human liver ECM with topographically featured electrospun scaffolds for engineering hepatic microenvironment

Combining Human Liver ECM with Topographically Featured Electrospun Scaffolds for Hybrid Hepatic Models

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