Effects of elevated temperature on 8-OHdG expression in the American oyster (Crassostrea virginica): Induction of oxidative stress biomarkers, cellular apoptosis, DNA damage and γH2AX signaling pathways

Rahman, Md. Mushfiqur; Billah, Mohammad Maruf; Kline, Richard J.; Rahman, Md. Saydur

doi:10.1016/j.fsirep.2022.100079

Cited by 10 publications

(13 citation statements)

References 129 publications

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“…In nuclear and mitochondrial DNA, 8-OHdG is the result of oxidative DNA damage when deoxyguanosine, a component of DNA, is oxidized. 47 In our study, we also found that fucoxanthin attenuated TBI-induced oxidative DNA damage in ECs as evidenced by 8-OHdG/CD31 double staining.…”

Section: Discussionsupporting

confidence: 76%

Fucoxanthin ameliorates traumatic brain injury by suppressing the blood–brain barrier disruption

Zhang,

Hu,

Bai

et al. 2023

iScience

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Section: Discussionsupporting

confidence: 76%

Fucoxanthin ameliorates traumatic brain injury by suppressing the blood–brain barrier disruption

Zhang,

Hu,

Bai

et al. 2023

iScience

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“…Herein, the oxidative brain injury induced by exposure to WBH extended to induce oxidative DNA damage that was reflected by the increase of 8-OHdG levels in the brain of the hyperthermic rats. In the same line, heat shock-driven oxidative stress induced DNA damage and increased 8-OHdG level in cells/tissues in oysters (Rahman et al, 2022). Moreover, increased serum 8-OHdG level was observed in high temperature-exposed bakery workers (Kheder and Al-Dosky, 2023).…”

Section: Discussionmentioning

confidence: 78%

Whole Body Hyperthermia-Induced Brain Injury in Rats: Forensic Biochemical, Pathological, and Immunohistochemical Investigations

El-Borai,

El-Bahrawy,

Assayed

et al. 2023

Journal of Current Veterinary Research

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Episodes of hyperthermia occur frequently from exposure to infections or adverse climatic changes. Such hyperthermia has been linked to various detrimental health effects. This study aimed to investigate the pathophysiological responses to acute versus subacute whole-body hyperthermia (WBH) in rats, particularly on the brain, with special reference to their medicolegal importance. Rats were randomly assigned to three equal groups. The normothermic group was kept at room temperature, the acute whole-body hyperthermic (AWBH) group was subjected to long-period WBH as a single bout of 4 hours at 43 °C, and the subacute whole-body hyperthermic (SWBH) group was subjected to repeated short-period WBH;2 hours daily for 7 successive days at 43 °C. A significant increase in body temperature and a significant decrease in body weight were recorded in both the acute and subacute hyperthermic groups. However, significant elevations in the serum glucose and cortisol levels and in the brain malondialdehyde and 8-hydroxy-2'-desoxyguanosine levels, with a significant reduction in brain total antioxidant capacity, were observed only in the AWBH group. Moreover, exposure to WBH induced various degrees of pathological changes, along with positive immune reactivity for heat shock protein 70 and glial fibrillary acidic protein in the cerebrum and cerebellum of rats in both hyperthermic groups. Overall, WBH induced various adverse health effects, mediated by the induction of oxidative damage, particularly following acute exposure. Hence, these findings provide evidence that acute exposure to long-term WBH may markedly exacerbate brain injury more than repeated short-term exposure, reflecting the role of the adaptive mechanisms to repeated heat exposure. Interestingly, the obtained findings may be valuable in the forensic antemortem and postmortem diagnosis of WBH and heat-related deaths.

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“…The DNA oxidative damage is usually characterized by 8-OHdG, which is the product of DNA oxidation from deoxyguanosine. 46 No obvious difference in the expression of 8-OHdG was observed in both groups at 12 h (Figure 7A). With the entrance of the decline phase, the expression of 8-OHdG in air-incubated S. putrefaciens exhibited an obvious increase at 72 h (Figure 7A).…”

Section: Synthesis and Activity Of Extracellular Proteasementioning

confidence: 92%

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

Li,

Wang,

Xie

2024

J. Agric. Food Chem.

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To explore whether oxidative stress caused by 100% CO 2 is an inhibitory mechanism against Shewanella putrefaciens, the oxidative stress reaction, antioxidant activity, and damage to the cell membrane, protein, and DNA of CO 2 -incubated S. putrefaciens at 4 °C were evaluated. Research demonstrated that CO 2 caused more severe reactive oxygen species (ROS) accumulation. Simultaneously, weaker•− -scavenging activity and decreased T-VOC and GSH content were also observed. The activities of antioxidant enzymes (SOD, POD, CAT, and GPX) continuously declined, which might be attributed to the CO 2 -mediated decrease in the pH value. Correspondingly, the cell membrane was damaged with hyperpolarization, increased permeability, and more severe lipid peroxidation. The expression of total and membrane protein decreased, and the synthesis and activity of extracellular protease were inhibited. DNA was also subjected to oxidative damage and expressed at a lower level. All results collaboratively confirmed that ROS excitation and inhibition of antioxidant activity were important inhibition mechanisms of CO 2 on S. putrefaciens.

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Effects of elevated temperature on 8-OHdG expression in the American oyster (Crassostrea virginica): Induction of oxidative stress biomarkers, cellular apoptosis, DNA damage and γH2AX signaling pathways

Cited by 10 publications

References 129 publications

Fucoxanthin ameliorates traumatic brain injury by suppressing the blood–brain barrier disruption

Fucoxanthin ameliorates traumatic brain injury by suppressing the blood–brain barrier disruption

Whole Body Hyperthermia-Induced Brain Injury in Rats: Forensic Biochemical, Pathological, and Immunohistochemical Investigations

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

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Effects of elevated temperature on 8-OHdG expression in the American oyster (Crassostrea virginica): Induction of oxidative stress biomarkers, cellular apoptosis, DNA damage and γH2AX signaling pathways

Cited by 10 publications

References 129 publications

Fucoxanthin ameliorates traumatic brain injury by suppressing the blood–brain barrier disruption

Fucoxanthin ameliorates traumatic brain injury by suppressing the blood–brain barrier disruption

Whole Body Hyperthermia-Induced Brain Injury in Rats: Forensic Biochemical, Pathological, and Immunohistochemical Investigations

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO2 on Shewanella putrefaciens at 4 °C

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Product

Resources

About

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C