Effects of endogenous substance P expression on degranulation in RBL-2H3 cells

Hu, Hua; Zhang, Ruxin; Fang, Xiaoyun; Yu, Min; Yu, Shui; Zhang, Jie; Wang, Hong

doi:10.1007/s00011-010-0301-6

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…In line with this, Berger et al 38 found a coregulated decrease of TAC4 mRNA and TACR1 (that encode TK receptor 1) mRNA as well as their proteins in monocytes and macrophages following the activation by monocytic differentiation cytokines. It is also noteworthy that SP has been shown to be increased in activated mass cells, and knocking down of endogenous SP reduced the degranulation ability of the cells 39 . These results clearly support the role of TKs, especially HK‐1 and SP, in different stages of immunomodulatory processes, which can assist in developing more‐effective therapeutic approaches by introducing these new targets to treat immune disorders.…”

Section: Immune Cell Development and Functionssupporting

confidence: 58%

The immunomodulatory effects of tachykinins and their receptors

Khorasani

Boroumand

Arab

et al. 2020

J of Cellular Biochemistry

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Tachykinins (TKs) are a family of neuropeptides mainly expressed by neuronal and non-neuronal cell types, especially immune cells. Expression of TKs receptors on immune cell surfaces, their involvement in immune-related disorders, and therefore, understanding their immunomodulatory roles have become of particular interest to researchers. In fact, the precise understanding of TKs intervention in the immune system would help to design novel therapeutic approaches for patients suffering from immune disorders. The present review summarizes studies on TKs function as modulators of the immune system by reviewing their roles in generation, activation, development, and migration of immune cells. Also, it discusses TKs involvement in three main cellular mechanisms including inflammation, apoptosis, and proliferation. K E Y W O R D S immune system, immunomodulation, tachykinin, tachykinin receptor 3032 |

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Section: Immune Cell Development and Functionssupporting

confidence: 58%

The immunomodulatory effects of tachykinins and their receptors

Khorasani

Boroumand

Arab

et al. 2020

J of Cellular Biochemistry

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“…Similarly, we also observed the upregulation of SP levels in synovial tissues obtained from patients with different stages of DDH compared with those obtained from the control group. It has been reported that SP is involved in the mediation of inflammatory hyperalgesia and immune regulation [ 40 ]. In this study, we explored the possible correlation between SP and inflammatory cytokines in the SF of patients with severe DDH.…”

Section: Discussionmentioning

confidence: 99%

Increasing expression of substance P and calcitonin gene-related peptide in synovial tissue and fluid contribute to the progress of arthritis in developmental dysplasia of the hip

Wang

Zhang

et al. 2015

Arthritis Res Ther

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IntroductionDevelopmental dysplasia of the hip (DDH) is a common musculoskeletal disorder that has pain and loss of joint function as major pathological features. In the present study, we explored the mechanisms of possible involvement and regulation of substance P (SP) and calcitonin gene-related peptide (CGRP) in the pathological and inflammatory processes of arthritis in DDH.MethodsBlood, synovial tissue and fluid samples were collected from patients diagnosed with different severities of DDH and from patients with femoral neck fracture. Levels of SP, CGRP and inflammatory cytokines in synovium and synovial fluid (SF) in the different groups were evaluated by immunohistochemistry, real-time PCR and enzyme-linked immunosorbent assay (ELISA). Correlations between neuropeptides and inflammatory cytokines in SF were evaluated by partial correlation analysis. The proinflammatory effects of SP and CGRP on synoviocytes obtained from patients with moderate DDH were investigated in vitro by real-time PCR and ELISA. The mechanisms of those effects were evaluated by Western blot analysis and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) DNA binding assay.ResultsSignificantly increased levels of neuropeptides and inflammatory cytokines were observed in synovium and SF from patients in the severe DDH group compared with the moderate DDH and control groups. In moderate DDH samples, SP in SF correlated with tumor necrosis factor (TNF)-α, and CGRP in SF correlated with TNF-α and interleukin (IL)-10. In the severe DDH group, SP in SF correlated with interleukin (IL)-1β, TNF-α and IL-10. CGRP in SF correlated with TNF-α. Additionally, SP might have had obvious proinflammatory effects on synoviocytes through the activation of NF-κB.ConclusionsThe upregulation of SP and CGRP in synovium and SF might participate in the inflammatory process of arthritis in DDH. The activation of the NF-κB pathway seems indispensable in the proinflammatory effect of SP on synoviocytes. This original discovery may indicate a potential clinical drug target and the development of innovative therapies for DDH.

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“…There is some evidence that the expression of endogenous SP mRNA and peptide is significantly increased under IgE-activated conditions and that small hairpin RNA (shRNA)- mediated knockdown of endogenous SP can reduce the ability of IgE-activated mast cells to undergo degranulation. 240 This finding suggests that endogenous SP plays an important role in mast cell antigen-mediated degranulation and thus enhances the progression of allergic inflammation. 240 Indeed, it is notable that SP is expressed not only by neurons, but also in immune cells such as mast cells 240 which release allergic mediators such as histamine, 241 thereby exacerbating allergic symptoms.…”

Section: Mechanismsmentioning

confidence: 98%

“… 240 This finding suggests that endogenous SP plays an important role in mast cell antigen-mediated degranulation and thus enhances the progression of allergic inflammation. 240 Indeed, it is notable that SP is expressed not only by neurons, but also in immune cells such as mast cells 240 which release allergic mediators such as histamine, 241 thereby exacerbating allergic symptoms. It is possible that widespread expression of SP in diverse cell types may indicate a multifunctional role for SP in a wide variety of physiologic and pathophysiologic conditions by activating a multitude of signalling pathways.…”

Section: Mechanismsmentioning

confidence: 98%

Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis

Cheng

Chen

et al. 2018

Allergy Asthma Immunol Res

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261

198

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Allergic rhinitis (AR) is a global health problem that causes major illnesses and disabilities worldwide. Epidemiologic studies have demonstrated that the prevalence of AR has increased progressively over the last few decades in more developed countries and currently affects up to 40% of the population worldwide. Likewise, a rising trend of AR has also been observed over the last 2–3 decades in developing countries including China, with the prevalence of AR varying widely in these countries. A survey of self-reported AR over a 6-year period in the general Chinese adult population reported that the standardized prevalence of adult AR increased from 11.1% in 2005 to 17.6% in 2011. An increasing number of original articles and imporclinical trials on the epidemiology, pathophysiologic mechanisms, diagnosis, management and comorbidities of AR in Chinese subjects have been published in international peer-reviewed journals over the past 2 decades, and substantially added to our understanding of this disease as a global problem. Although guidelines for the diagnosis and treatment of AR in Chinese subjects have also been published, they have not been translated into English and therefore not generally accessible for reference to non-Chinese speaking international medical communities. Moreover, methods for the diagnosis and treatment of AR in China have not been standardized entirely and some patients are still treated according to regional preferences. Thus, the present guidelines have been developed by the Chinese Society of Allergy to be accessible to both national and international medical communities involved in the management of AR patients. These guidelines have been prepared in line with existing international guidelines to provide evidence-based recommendations for the diagnosis and management of AR in China.

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Effects of endogenous substance P expression on degranulation in RBL-2H3 cells

Abstract: Activated RBL-2H3 cells express endogenous SP which plays a role in antigen-induced degranulation.

Cited by 6 publications

References 30 publications

The immunomodulatory effects of tachykinins and their receptors

The immunomodulatory effects of tachykinins and their receptors

Increasing expression of substance P and calcitonin gene-related peptide in synovial tissue and fluid contribute to the progress of arthritis in developmental dysplasia of the hip

Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis

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