2002
DOI: 10.1677/joe.0.1750225
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Effects of endothelin-1 on release of adrenomedullin and C-type natriuretic peptide from individual human vascular endothelial cells

Abstract: Regulation of cardiovascular system activity involves complex interactions amongst numerous factors. Three of these vasoactive factors are adrenomedullin, C-type natriuretic peptide (CNP) and endothelin-1 (ET-1), each of which is claimed to have important local effects. To investigate paracrine/autocrine regulation of the secretion of these peptides we used a cell immunoblot method. We postulated that basal release of adrenomedullin and CNP by endothelial cells is modulated by ET-1. Dispersed human aortic endo… Show more

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Cited by 18 publications
(10 citation statements)
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“…In this study, a significant increase in adrenomedullin-secreting cells was detected at 0$001 mM angiotensin and maximum stimulation was observed at about 1 mM. Previous experiments in our laboratory have indicated that endothelin-1 increases the number of cells secreting adrenomedullin (Evans et al 2002). It is evident therefore that adrenomedullin is released from endothelial cells in response to two potent vasoconstrictors, angiotensin-II and endothelin-1.…”
Section: Discussionsupporting
confidence: 61%
See 1 more Smart Citation
“…In this study, a significant increase in adrenomedullin-secreting cells was detected at 0$001 mM angiotensin and maximum stimulation was observed at about 1 mM. Previous experiments in our laboratory have indicated that endothelin-1 increases the number of cells secreting adrenomedullin (Evans et al 2002). It is evident therefore that adrenomedullin is released from endothelial cells in response to two potent vasoconstrictors, angiotensin-II and endothelin-1.…”
Section: Discussionsupporting
confidence: 61%
“…The cell immunoblot method, in which cells are incubated on a protein-binding membrane, has been extensively employed by our laboratory (Evans et al 2002(Evans et al , 2003 and others (Arita 1993, Kusaka et al 2000. The method uses singly dispersed cells, and in contrast to other methods in which cells are cultured in high density, is able to reduce effects of paracrine interactions, and can investigate functional diversity which may be present in endothelial cells (Frid et al 2004).…”
Section: Introductionmentioning
confidence: 99%
“…This result is in agreement with several previous findings. Thus, both ETA and ETA/ETB receptor antagonists decrease plasma atrial natriuretic peptide responses to volume overload (22); the nonselective endothelin receptor antagonist bosentan blocks the increase in Nppb mRNA levels produced by pressure overload in the left atria (23), and antiendothelin-1 serum decreases the percentage of cells that secrete C-type natriuretic peptides in human vascular endothelial cells (24). Together, these studies suggest that below-normal expression of Edn1 probably suppresses the increased expression of natriuretic peptides that is the normal response to volume expansion.…”
Section: Discussionmentioning
confidence: 99%
“…The method was based on that previously described [24]. Briefly, HAEC were transferred to glass cloning rings sealed to an Immobilon-P protein binding membrane and incubated in DMEM-H, EBM 0.1% BSA only or EBM with either 0.025, 0.25, or 0.75 mM H 2 O 2 for 12 hours.…”
Section: Determination Of Cells Secreting Imd Peptidementioning
confidence: 99%