Effects of Enterally Administering Granulocyte Colony-Stimulating Factor to Suckling Mice

Gersting, Jason A.; Christensen, Robert D.; Calhoun, Darlene A

doi:10.1203/01.pdr.0000117846.51197.7c

Cited by 17 publications

(8 citation statements)

References 41 publications

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“…Feeding rhG-CSF to suckling mice resulted in a larger villous area, a longer villous perimeter, a longer villous length, an increased crypt depth, and increased mitotic activity as estimated by the PCNA (proliferating cell nuclear antigen) index, [33]. Previous studies demonstrated that G-CSF inhibited BT to the abdominal organs in rats and mongrel dogs with severe acute pancreatitis [34,35].…”

Section: Discussionmentioning

confidence: 99%

Confirmation and Prevention of Intestinal Barrier Dysfunction and Bacterial Translocation Caused by Methotrexate

et al. 2006

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When intestinal barrier function is damaged bacterial translocation (BT) can occur. The injury to intestinal barrier function caused by chemotherapy has been investigated in some studies, however, definitive evidence of BT caused by chemotherapy is lacking. The aim of this study was to investigate small intestinal barrier dysfunction and BT and to evaluate the preventive effect of granulocyte colony-stimulating factor (G-CSF) on intestinal barrier dysfunction and BT in a rat model of chemotherapy. Sprague-Dawley rats were treated with methotrexate (MTX; 3.5 mg/kg) for 3 days to induce intestinal barrier dysfunction and BT, gavaged Escherichia coli TG1 labeled with green fluorescent protein for 2 days to track BT, and G-CSF (10 microg/kg) for 4 days to prevent intestinal barrier dysfunction and BT. Intestinal permeability was measured by the urinary excretion rate of lactulose and mannitol following administration by gavage. Representative tissue specimens from the mesenteric lymph nodes, spleen, liver, and kidney were aseptically harvested for bacteria culture in ampicillin-supplemented medium. Light microscopy was performed on intestinal samples. MTX induced significant mucosal and villous atrophy in ileum and significantly increased intestinal permeability. MTX also induced noticeable BT. G-CSF significantly increased the mucosal thickness and villous height of the ileum and decreased intestinal permeability and BT. In conclusion, MTX caused intestinal barrier dysfunction and BT, and G-CSF prevented intestinal barrier dysfunction and BT.

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Section: Discussionmentioning

confidence: 99%

Confirmation and Prevention of Intestinal Barrier Dysfunction and Bacterial Translocation Caused by Methotrexate

et al. 2006

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“…While milk-borne G-CSF survives transit through the stomach, it is not appreciably absorbed and acts at the intestinal surface , 106,107 where it increases villi, crypt depth, and cell proliferation. 108 Other regulatory cytokines found in milk are IL-10 and IL-7; 109 milk-borne IL-7 is known to cross the intestinal wall and influence thymic development. 110 …”

Section: Immunological Factorsmentioning

confidence: 99%

Human Milk Composition

Ballard¹,

Morrow²

2013

Pediatric Clinics of North America

2,021

922

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Synopsis The composition of human milk is the biologic norm for infant nutrition. Human milk also contains many hundreds to thousands of distinct bioactive molecules that protect against infection and inflammation and contribute to immune maturation, organ development, and healthy microbial colonization. Some of these molecules, e.g., lactoferrin, are being investigated as novel therapeutic agents. A dynamic, bioactive fluid, human milk changes in composition from colostrum to late lactation, and varies within feeds, diurnally, and between mothers. Feeding infants with expressed human milk is increasing. Pasteurized donor milk is now commonly provided to high risk infants and most mothers in the U.S. express and freeze their milk at some point in lactation for future infant feedings. Many milk proteins are degraded by heat treatment and freeze-thaw cycles may not have the same bioactivity after undergoing these treatments. This article provides an overview of the composition of human milk, sources of its variation, and its clinical relevance.

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“…66 These cytokines are present in amniotic fluid and human milk, are swallowed by the fetus in large amounts, 199,200 and have a demonstrated an in vitro and in vivo protective effect on gut mucosa. [201][202][203][204][205][206]…”

Section: Recombinant Cytokinesmentioning

confidence: 99%