Effects of environmental factors on denitrifying bacteria and functional genes in sediments of Bohai Sea, China

Chen, Quanrui; Fan, Jing-Li; Ming, Hongxia; Su, Jie; Wang, Yantao; Wang, Bin

doi:10.1016/j.marpolbul.2020.111621

Cited by 25 publications

(11 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nitrate can be reduced into nitrogen through denitrifying bacteria. The nitrogen metabolism pathway indicates that functional denitrification genes such as nirS (playing an important role in nitrite reduction), norB , nosZ , and narG [ 72 ] are detected in P. stutzeri with denitrification. In addition, nitrate is metabolized into ammonia through dissimilatory nitrate reduction, and the annotated gene nirBD encodes nitrite reductase (EC:1.7.1.15) and can contribute to nitrite reduction [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

et al. 2022

View full text Add to dashboard Cite

Background Pseudomonas stutzeri S116 is a sulfur-oxidizing bacteria isolated from marine sludge. It exhibited excellent electricity generation as bioanode and biocathode applied in microbial fuel cells (MFCs). Complete genome sequencing of P. stutzeri and cyclic voltammetry method were performed to reveal its mechanism in microbial fuel cells system. Results This study indicated that the MFCs generated a maximum output voltage of 254.2 mV and 226.0 mV, and maximum power density of 765 mW/m2 and 656.6 mW/m2 respectively. Complete genome sequencing of P. stutzeri S116 was performed to indicate that most function genes showed high similarities with P. stutzeri, and its primary annotations were associated with energy production and conversion (6.84%), amino acid transport and metabolism (6.82%) and inorganic ion transport and metabolism (6.77%). Homology of 36 genes involved in oxidative phosphorylation was detected, which suggests the strain S116 possesses an integrated electron transport chain. Additionally, many genes encoding pilus-assembly proteins and redox mediators (riboflavin and phenazine) were detected in the databases. Thiosulfate oxidization and dissimilatory nitrate reduction were annotated in the sulfur metabolism pathway and nitrogen metabolism pathway, respectively. Gene function analysis and cyclic voltammetry indicated that P. stutzeri probably possesses cellular machinery such as cytochrome c and redox mediators and can perform extracellular electron transfer and produce electricity in MFCs. Conclusion The redox mediators secreted by P. stutzeri S116 were probably responsible for performance of MFCs. The critical genes and metabolic pathways involved in thiosulfate oxide and nitrate reduction were detected, which indicated that the strain can treat wastewater containing sulfide and nitrite efficiently.

show abstract

Section: Discussionmentioning

confidence: 99%

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Although the biogeochemical cycling driven by microbes has displayed instant or delayed response to oxygen depletion ( Bertics et al, 2013 ; Sinkko et al, 2013 ), less is known for the microbial communities in the reported oxygen depletion water of Bohai sea. The contribution of denitrifying bacteria in Bohai Sea has been revealed by denitrification rate, relative abundance, and NO 3 –/ nitrite (NO 2 – ) reductase activity of nar / nir -encoded denitrifiers in sediments ( Wang et al, 2015 ; Zhang et al, 2019 ; Chen et al, 2020 ). However, information on the nosZ -type denitrifying bacteria and their metabolism capability in Bohai Sea water column is still unknown, and the potential correlation between denitrifying bacteria and the formation of the bottom water hypoxia is also awaiting to discover.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Variation of Bacteria in Response to Summertime Oxygen Depletion in Water Column of Bohai Sea

Wang

Guo

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Aiming to reveal the variation in bacteria community under oxygen depletion formed every summer in water column of central Bohai Sea, a time-scenario sampling from June to August in 2018 at a 20-day interval along one inshore–offshore transect was settled. Water samples were collected at the surface, middle, and bottom layer and then analyzed by high-throughput sequencing targeting both 16S rRNA and nosZ genes. Compared to the surface and middle water, oxygen depletion occurred at bottom layer in August. In top two layers, Cyanobacteria dominated the bacterial community, whereas heterotrophic bacteria became dominant in bottom water of Bohai Sea. Based on the time scenario, distinct community separation was observed before (June and July) and after (August) oxygen depletion (p = 0.003). Vertically, strict stratification of nosZ gene was stably formed along 3 sampling layers. As a response to oxygen depletion, the diversity indices of both total bacteria (16S rRNA) and nosZ gene-encoded denitrification bacteria all increased, which indicated the intense potential of nitrogen lose when oxygen depleted. Dissolved oxygen (DO) was the key impacting factor on the community composition of total bacteria in June, whereas nutrients together with DO play the important roles in August for both total and denitrifying bacteria. The biotic impact was revealed further by strong correlations which showed between Cyanobacteria and heterotrophic bacteria in June from co-occurrence network analysis, which became weak in August when DO was depleted. This study discovered the variation in bacteria community in oxygen-depleted water with further effort to understand the potential role of denitrifying bacteria under oxygen depletion in Bohai Sea for the first time, which provided insights into the microbial response to the world-wide expanding oxygen depletion and their contributions in the ocean nitrogen cycling.

show abstract

“…Therefore, DO is also a primary factor in developing the bacterioplankton community structure [ 69 ]. In addition, TP is one of the determinants of bacterial community structure in sediments, which could change bacteria’s overall structure and function by affecting denitrifying bacteria [ 70 , 71 ]. The co-occurrence networks revealed that COD Mn , Chl a , and pH were associated with multiple nodes in the bacterioplankton network, and a few dominant genera were associated with TP in another network.…”

Section: Discussionmentioning

confidence: 99%

Determinants and Assembly Mechanism of Bacterial Community Structure in Ningxia Section of the Yellow River

et al. 2023

View full text Add to dashboard Cite

The Yellow River is a valuable resource in the Ningxia Hui Autonomous Region and plays a vital role in local human activities and biodiversity. Bacteria are a crucial component of river ecosystems, but the driving factors and assembly mechanisms of bacterial community structure in this region remain unclear. Herein, we documented the bacterial community composition, determinants, co-occurrence pattern, and assembly mechanism for surface water and sediment. In comparison to sediment, the bacterioplankton community showed significant seasonal variation, as well as less diversity and abundance. The network topology parameters indicated that the sediment bacterial network was more stable than water, but the bacterioplankton network had higher connectivity. In this lotic ecosystem, CODMn, Chl a, and pH affected the structure of the bacterioplankton community, while TP was the primary factor influencing the structure of the sediment bacterial community. The combined results of the neutral community model and the phylogenetic null model indicate that Bacterial communities in both habitats were mainly affected by stochastic processes, with ecological processes dominated by ecological drift for bacterioplankton and dispersal limitation for sediment bacteria. These results provide essential insights into future research on microbial ecology, environmental monitoring, and classified management in the Ningxia section of the Yellow River.

show abstract

Effects of environmental factors on denitrifying bacteria and functional genes in sediments of Bohai Sea, China

Cited by 25 publications

References 52 publications

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

Understanding the Variation of Bacteria in Response to Summertime Oxygen Depletion in Water Column of Bohai Sea

Determinants and Assembly Mechanism of Bacterial Community Structure in Ningxia Section of the Yellow River

Contact Info

Product

Resources

About