Effects of environmentally relevant concentrations of diclofenac in Mytilus trossulus

Świacka, Klaudia; Smolarz, Katarzyna; Maculewicz, Jakub; Caban, Magda

doi:10.1016/j.scitotenv.2020.139797

Cited by 21 publications

(8 citation statements)

References 62 publications

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“…The effects of the drug in the present and other studies (wiacka et al, 2020) were related to its concentration in water. The present study did not reveal effects on all studied functions at levels of diclofenac from 0.001 to 0.1 μg/L, which can be considered a no‐observed‐effect concentration (NOEC) for benthic invertebrates under conditions of short‐term exposure.…”

Section: Discussionsupporting

confidence: 68%

“…The percentage of diclofenac absorption from water to soft tissues depends on the concentration in the environment (Jodeh et al, 2016). The bivalve mollusk Mytilus can absorb 4–40 μg/L of diclofenac from the environment into its tissues so that the highest recorded concentration of diclofenac in body tissues was 1.69 μg/kg dry weight in 1 week (wiacka et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Effects of Diclofenac on the Reproductive Health, Respiratory Rate, Cardiac Activity, and Heat Tolerance of Aquatic Animals

Berezina

Sharov

Chernova

et al. 2021

Environmental Toxicology and Chemistry

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Diclofenac is an important pharmaceutical present in the water cycle of wastewater treatment and one of the most distributed drugs in aquatic ecosystems. Despite the great interest in the fate of diclofenac in freshwaters, the effects of environmentally relevant concentrations on invertebrates are still unclear. Two species of freshwater invertebrates, the amphipod Gmelinoides fasciatus and the bivalve mollusk Unio pictorum, were exposed to diclofenac concentrations of 0.001–2 μg/L (environmentally relevant levels) for 96 h. A set of biological endpoints (survival, fecundity, embryo abnormalities, respiration and heart rates, heat tolerance, and cardiac stress tolerance) were estimated in exposed invertebrates. Effects of diclofenac on amphipod metabolic rate and reproduction (number and state of embryos) and adaptive capacity (cardiac stress tolerance) in both species were evident. The oxygen consumption of amphipods exposed to diclofenac of 0.1–2 μg/L was 1.5–2 times higher than in the control, indicating increased energy requirements for standard metabolism in the presence of diclofenac (>0.1 μg/L). The heart rate recovery time in mollusks after heating to critical temperature (30 °C) was 1.7 and 9 times greater in mollusks exposed to 0.1 and 0.9 μg/L, respectively, than in the control (24 min). A level of diclofenac >0.9 μg/L adversely affected amphipod embryos, leading to an increase in the number of embryos with impaired development, which subsequently died. Thus, the lowest effective concentration of diclofenac (0.1 μg/L) led to increased energy demands of animals while reducing cardiac stress tolerance, and at a level close to 1 μg/L reproductive disorders (elevated mortality of the embryos) occurred. Environ Toxicol Chem 2022;41:677–686. © 2021 SETAC

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Effects of Diclofenac on the Reproductive Health, Respiratory Rate, Cardiac Activity, and Heat Tolerance of Aquatic Animals

Berezina

Sharov

Chernova

et al. 2021

Environmental Toxicology and Chemistry

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“…Special attention has been paid in the last years to the presence of pharmaceutical compounds in the aquatic environment because of their biological activity, pseudo-persistence, bioaccumulation properties, and toxicity [ 1 – 3 ]. They are continuously discharged from wastewater treatment plants (WWTPs) [ 1 , 4 ] and can be accumulated [ 2 , 5 – 10 ] and trophic magnified [ 11 ] in aquatic organisms affecting not only the aquatic fauna [ 1 , 12 , 13 ] but also human health through the consumption of edible aquatic organisms [ 11 , 14 ]. Their identification in marine bivalves is an issue of special interest as they are filter feeders that can accumulate pollutants present in the aquatic media [ 15 – 17 ] what makes them useful indicators of water pollution [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-assisted extraction as an easy-to-perform analytical methodology for monitoring ibuprofen and its main metabolites in mussels

et al. 2022

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Non-steroidal anti-inflammatory drugs (NSAIDs) have been reported to be the main pharmaceutical class accumulated in seafood. Among them, ibuprofen (IBU) is of special concern as it is used worldwide to treat common pain, does not require a medical prescription, it is often taken in a high daily dose, and has been reported to cause potential adverse effects on aquatic organisms. IBU is highly transformed into hydroxy- and carboxy-metabolites and/or degradation products generated not only after its administration but also during wastewater treatment or in the environment. These compounds can be present in the environment at higher concentrations than IBU and present higher toxicity. In this work, a low-cost and affordable routine analytical method was developed and validated for the first-time determination of IBU and its main metabolites in mussels. The method is based on ultrasound-assisted extraction (UAE), clean-up by dispersive solid-phase extraction (d-SPE) and analytical determination by liquid chromatography-tandem mass spectrometry. Box-Behnken experimental design was used for method optimisation to better evaluate the influence and interactions of UAE and d-SPE variables. Extraction recoveries were in the range from 81 to 115%. Precision, expressed as relative standard deviation, was lower than 7%. Method detection limits were in the range from 0.1 to 1.9 ng g−1 dry weight. The method was successfully applied to wild mussels. Graphical abstract

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“…Not surprisingly, studies have shown that many pharmaceuticals have been detected in rivers (ng/L−μg/L), , aquatic vertebrates (e.g., fish), and invertebrates (e.g., gammarids) (ng/g−μg/g). − Diclofenac as a widely used NSAID in both humans and livestock, has been widely found in various environmental media, including in the ng/L to μg/L range in surface waters and ng/g to μg/g range in aquatic organisms. ,, Even though the detected concentrations are low, pharmaceuticals may pose a risk to the nontarget organisms in the aquatic ecosystems because they are designed to be biologically active at low doses for targeted organisms (e.g., humans) and often have a specific mechanism of action (MoA). For example, the pharmacological MoA of diclofenac in humans is the reduction of prostaglandin synthesis by inhibiting cyclooxygenase-1 (COX-1) and COX-2. , Interestingly, a few studies have shown that diclofenac also affects the prostaglandin metabolome in several nontarget organisms such as rainbow trout ( Oncorhynchus mykiss ), zebrafish ( Danio rerio ), and marine mussels ( Mytilus galloprovincialis ) by using metabolomics. , Also, studies have shown that exposure of diclofenac induces adverse effects in different aquatic vertebrates and invertebrates, ,,− as exemplified by the toxic effects in different organs (e.g., cytological alterations in the liver, kidney, and gills) of rainbow trout exposed to environmentally relevant concentrations (1–5 μg/L) …”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling and Toxicokinetics Modeling to Assess the Effects of the Pharmaceutical Diclofenac in the Aquatic Invertebrate Hyalella azteca

Scheidegger

Laczkó

et al. 2021

Environ. Sci. Technol.

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The exposure of ecologically critical invertebrate species to biologically active pharmaceuticals poses a serious risk to the aquatic ecosystem. Yet, the fate and toxic effects of pharmaceuticals on these nontarget aquatic invertebrates and the underlying mechanisms are poorly studied. Herein, we investigated the toxicokinetic (TK) processes (i.e., uptake, biotransformation, and elimination) of the pharmaceutical diclofenac and its biotransformation in the freshwater invertebrate Hyalella azteca. We further employed mass spectrometry-based metabolomics to assess the toxic effects of diclofenac on the metabolic functions of H. azteca exposed to environmentally relevant concentrations (10 and 100 μg/L). The TK results showed a quick uptake of diclofenac by H. azteca (maximum internal concentration of 1.9 μmol/kg) and rapid formation of the conjugate diclofenac taurine (maximum internal concentration of 80.6 μmol/kg), indicating over 40 times higher accumulation of diclofenac taurine than that of diclofenac in H. azteca. Depuration kinetics demonstrated that the elimination of diclofenac taurine was 64 times slower than diclofenac in H. azteca. Metabolomics results suggested that diclofenac inhibited prostaglandin synthesis and affected the carnitine shuttle pathway at environmentally relevant concentrations. These findings shed light on the significance of the TK process of diclofenac, especially the formation of diclofenac taurine, as well as the sublethal effects of diclofenac on the bulk metabolome of H. azteca. Combining the TK processes and metabolomics provides complementary insights and thus a better mechanistic understanding of the effects of diclofenac in aquatic invertebrates.

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Effects of environmentally relevant concentrations of diclofenac in Mytilus trossulus

Cited by 21 publications

References 62 publications

Effects of Diclofenac on the Reproductive Health, Respiratory Rate, Cardiac Activity, and Heat Tolerance of Aquatic Animals

Effects of Diclofenac on the Reproductive Health, Respiratory Rate, Cardiac Activity, and Heat Tolerance of Aquatic Animals

Ultrasound-assisted extraction as an easy-to-perform analytical methodology for monitoring ibuprofen and its main metabolites in mussels

Metabolomic Profiling and Toxicokinetics Modeling to Assess the Effects of the Pharmaceutical Diclofenac in the Aquatic Invertebrate Hyalella azteca

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