Effects of Enzyme Types and Extraction Conditions on Protein Recovery and Antioxidant Properties of Hydrolysed Proteins Derived from Defatted Lemna minor

Tran, Hai Le; Le, Hong Anh Thi; Le, Thanh Thanh; Phan, Van Man

doi:10.14416/j.asep.2021.05.003

Cited by 6 publications

(6 citation statements)

References 9 publications

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“…In the first study, Tran et al performed enzymatic hydrolyses of proteins from defatted Lemna minor using flavourzyme and alcalase at different concentrations and times and they evaluated the antioxidant property of the hydrolysates. The DHs obtained varied between 9.45% and 16.34% [26], which is significantly higher than those obtained in the present study. These differences can be explained by different factors: the enzymes used for hydrolyses were not specific, the raw material was different (blend of several duckweed species from Florida vs. Lemna minor from Vietnam), differences in hydrolysis conditions (higher temperature, different durations and enzyme concentrations), and the methods used to measure the DH were different (OPA vs. TNBS assay).…”

Section: Degree Of Hydrolysis (Dh)contrasting

confidence: 88%

“…However, it is important to keep in mind that a higher degree of hydrolysis does not necessarily mean a more biologically interesting hydrolysate. To our knowledge, enzymatic hydrolysis of duckweed proteins has only been performed twice on duckweed proteins powder [26,27]. In the first study, Tran et al performed enzymatic hydrolyses of proteins from defatted Lemna minor using flavourzyme and alcalase at different concentrations and times and they evaluated the antioxidant property of the hydrolysates.…”

Section: Degree Of Hydrolysis (Dh)mentioning

confidence: 99%

“…Plant proteins serve as robust reservoirs of biologically active peptides and have prompted a considerable number of studies exploring the bioactivities spanning an extensive array of plant matrices [5,8,25]. Nonetheless, to the best of our knowledge, enzymatic hydrolysis of duckweeds has been carried out only twice to date [26,27], with these endeavors directed towards assessing the antioxidant, functional and antimicrobial attributes of the resulting hydrolysates. However, these studies did not characterize the peptide sequences present, and no studies reported the antihypertensive activities of duckweed hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic Hydrolysis of Water Lentil (Duckweed): An Emerging Source of Proteins for the Production of Antihypertensive Fractions

Bernier,

Thibodeau,

Bazinet

2024

Foods

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Water lentil (Duckweed), an emerging protein source, is a small floating aquatic plant with agronomic and compositional characteristics rendering it a potential source of bioactive peptides. However, enzymatic hydrolysis of duckweeds has only been carried out to assess the antioxidant and antimicrobial activities of the hydrolysates. The main objectives of this study were to perform enzymatic hydrolysis of duckweed powder utilizing several enzymes and to evaluate the final antihypertensive activity of the fractions. Duckweed powder was efficiently hydrolyzed by pepsin, chymotrypsin, papain and trypsin, with degree of hydrolysis ranging from 3% to 9%, even without prior extraction and concentration of proteins. A total of 485 peptide sequences were identified in the hydrolysates and only 51 were common to two or three hydrolysates. It appeared that phenolic compounds were released through enzymatic hydrolyses and primarily found in the supernatants after centrifugation at concentrations up to 11 mg gallic acid/g sample. The chymotryptic final hydrolysate, the chymotryptic supernatant and the papain supernatant increased the ACE inhibitory activity by more than 6- to 8-folds, resulting in IC50 values ranging between 0.55 to 0.70 mg peptides/mL. Depending on the fraction, the ACE-inhibition was attributed to either bioactive peptides, phenolic compounds or a synergistic effect of both. To the best of our knowledge, this was the first study to investigate the enzymatic hydrolysis of duckweed proteins to produce bioactive peptides with therapeutic applications in mind.

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Section: Degree Of Hydrolysis (Dh)contrasting

confidence: 88%

Section: Degree Of Hydrolysis (Dh)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzymatic Hydrolysis of Water Lentil (Duckweed): An Emerging Source of Proteins for the Production of Antihypertensive Fractions

Bernier,

Thibodeau,

Bazinet

2024

Foods

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“…Most studies also focused on the genus Lemna , although scant information is available on the biological and functional properties of the released peptides. Antimicrobial activity, ACE inhibitory, and DPPH scavenging activities were found for enzymatic hydrolysates obtained by W. globose , Lemna minor , and Chlorella sorokiniana proteins [ 257 , 258 ]. Microalgae peptides that mimic the functions of mediators involved in pathologic processes responsible for vascular damage playing a putative role in the prevention of cardiovascular disease were discussed by Li et al [ 212 ].…”

Section: Bioactive Properties Of Non-meat Proteinsmentioning

confidence: 99%

Alternative Protein Sources and Novel Foods: Benefits, Food Applications and Safety Issues

et al. 2023

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The increasing size of the human population and the shortage of highly valuable proteinaceous ingredients has prompted the international community to scout for new, sustainable, and natural protein resources from invertebrates (e.g., insects) and underutilized legume crops, unexploited terrestrial and aquatic weeds, and fungi. Insect proteins are known for their nutritional value, being rich in proteins with a good balance of essential amino acids and being a valuable source of essential fatty acids and trace elements. Unconventional legume crops were found rich in nutritional, phytochemical, and therapeutic properties, showing excellent abilities to survive extreme environmental conditions. This review evaluates the recent state of underutilized legume crops, aquatic weeds, fungi, and insects intended as alternative protein sources, from ingredient production to their incorporation in food products, including their food formulations and the functional characteristics of alternative plant-based proteins and edible insect proteins as novel foods. Emphasis is also placed on safety issues due to the presence of anti-nutritional factors and allergenic proteins in insects and/or underutilized legumes. The functional and biological activities of protein hydrolysates from different protein sources are reviewed, along with bioactive peptides displaying antihypertensive, antioxidant, antidiabetic, and/or antimicrobial activity. Due to the healthy properties of these foods for the high abundance of bioactive peptides and phytochemicals, more consumers are expected to turn to vegetarianism or veganism in the future, and the increasing demand for such products will be a challenge for the future.

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“…1 mg/ml of extract was dissolved in methanol, ethyl acetate, and hexane using different concentrations (25, 50, 75, and 100 μg/ml). For control, DPPH and methanol were used without the extract mixture [8]. The mixture was vigorously mixed and stored in the dark for 30 min.…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Paddy straw: an economical substrate for oyster mushroom (Pleurotus ostreatus) cultivation

Thomas

Arivu²,

Shanmugam³

et al. 2022

E3S Web Conf.

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Cultivation of the Pleurotus ostreatus, oyster mushroom on paddy straw without supplements was investigated to follow circular economy concept to convert agricultural waste to value added products. Substrate nutrients, mushroom yield, and biological efficiency were determined. Three different extracts were used in this study (methanol, ethyl acetate, and hexane). Antioxidant and scavenging activity was determined using DPPH and H2O2. To find the essential compounds present in the mushrooms, GC-MS was analyzed. It was found that mushroom growth on paddy substrate was less than five days with excess mushroom yield. The biological efficiency was found between 54.5-130.9%, with the moisture of 93%. It was found that C, P, N, and K were integrated into mushrooms with these elements than in the utilized substrate. In DPPH results, the minimum concentration was 37.07 μg/ml, and the maximum was 67.2 μg/ml. IC50 value of 42.6 μg/ml were 50% for inhibition concentration. In H2O2, the minimum concentration was found to be 72.57 μg/ml, and the maximum was 98.02 μg/ml. This concentration indicates that the IC50 value of 84.07 μg/ml can be used in the biological process or component by 50% for inhibition concentration. The compounds include Oxirane, 2-Methyl-3-(1-Methylethyl)-, O-Methylisourea Hydrogen Sulfate, Diethyl Phthalate, 1,1,3,3-Tetrapropoxy- were found commonly in all three extracts. Hence, analysis of mushroom extracts is needed to determine the mechanisms of action of the various components for antimicrobial activity and inhibitory activity. Therefore, paddy straw could be used as an effective and economical substrate for oyster mushroom cultivation.

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Effects of Enzyme Types and Extraction Conditions on Protein Recovery and Antioxidant Properties of Hydrolysed Proteins Derived from Defatted Lemna minor

Cited by 6 publications

References 9 publications

Enzymatic Hydrolysis of Water Lentil (Duckweed): An Emerging Source of Proteins for the Production of Antihypertensive Fractions

Enzymatic Hydrolysis of Water Lentil (Duckweed): An Emerging Source of Proteins for the Production of Antihypertensive Fractions

Alternative Protein Sources and Novel Foods: Benefits, Food Applications and Safety Issues

Paddy straw: an economical substrate for oyster mushroom (Pleurotus ostreatus) cultivation

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