Effects of Eosinophilotropic Cytokines on Differentiation of an Eosinophil Cell Line, YY-1

Ueno, Masao; Watanabe, Kimitsuna; Tsurufuji, Susumu; Hirashima, Mitsuomi

doi:10.1159/000236755

Cited by 3 publications

(5 citation statements)

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“…However, its expression is downregulated on mature human eosinophils when treated the same cocktail of cytokines (Gregory et al, 2003). Although the receptors are found on a wider range of cells, administration of IL-3 and GM-CSF has been shown to promote eosinophil production in animal models, as well as in clinical trials (Sanderson, 1992; Ueno et al, 1994; Takamoto and Sugane, 1995). …”

Section: Activation Of Eosinophils and Recruitment To The Allergic Lungmentioning

confidence: 99%

Eosinophils in Fungus-Associated Allergic Pulmonary Disease

Ghosh¹,

Hoselton²,

Dorsam³

et al. 2013

Front. Pharmacol.

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Asthma is frequently caused and/or exacerbated by sensitization to fungal allergens, which are ubiquitous in many indoor and outdoor environments. Severe asthma with fungal sensitization is characterized by airway hyperresponsiveness and bronchial constriction in response to an inhaled allergen that is worsened by environmental exposure to airborne fungi and which leads to a disease course that is often very difficult to treat with standard asthma therapies. As a result of complex interactions among inflammatory cells, structural cells, and the intercellular matrix of the allergic lung, patients with sensitization to fungal allergens may experience a greater degree of airway wall remodeling and progressive, accumulated pulmonary dysfunction as part of the disease sequela. From their development in the bone marrow to their recruitment to the lung via chemokine and cytokine networks, eosinophils form an important component of the inflammatory milieu that is associated with this syndrome. Eosinophils are recognized as complex multi-factorial leukocytes with diverse functions in the context of allergic fungal asthma. In this review, we will consider recent advances in our understanding of the molecular mechanisms that are associated with eosinophil development and migration to the allergic lung in response to fungal inhalation, along with the eosinophil’s function in the immune response to and the immunopathology attributed to fungus-associated allergic pulmonary disease.

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Section: Activation Of Eosinophils and Recruitment To The Allergic Lungmentioning

confidence: 99%

Eosinophils in Fungus-Associated Allergic Pulmonary Disease

Ghosh¹,

Hoselton²,

Dorsam³

et al. 2013

Front. Pharmacol.

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show abstract

“…The differential responsiveness of eosinophils isolated from patients or normal controls is still a puzzle. n-BA -induced maturation of YY-1 cells [21,22] leads to an increased IL-18R expression and the treated YY-1 cells could respond to IL-18. A fraction of peripheral eosinophils from HES patients could also be activated [28].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it is difficult to obtain sufficient eosinophils for functional analysis due to their small numbers in the peripheral blood cells. We therefore checked expression of IL-18R in the human eosinophilic cell lines, YY-1 [21,22] and EoL-1 [23] and other human cell lines including B cells (NALM6, RAMOS, DND39), myeloma cells (U266), T cells (Jurkat), monocytic cells (U937) and basophilic cells (KU812). As shown in Fig…”

Section: Expression Of Il-18 > Chain Receptor Mrna In Human Peripheramentioning

confidence: 99%

“…Since YY-1 cells are known to differentiate into more mature eosinophils following treatment with n-butyric acid (n-BA), as measured by the cellular contents of granules and cytokines [21,22], the effect of n-BA on the expression of § chain of IL-18R in YY-1 cells was studied by RT-PCR and FACS analyses. As shown in Fig.…”

Section: Treatment Of Yy-1 Cells With N-butyric Acid Enhances Expressmentioning

confidence: 99%

“…These observations prompted us to examine the effects of IL-18 on eosinophils. The expression of IL-18 § chain receptor was monitored on peripheral blood eosinophils and two human eosinophilic cell lines, YY-1 [21,22] and EoL-1 [23]. Incubation of YY-1 cells with IL-18 enhanced IL-8 synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Interleukin-18 enhances the production of interleukin-8 by eosinophils

et al. 2001

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Interleukin‐18 (IL‐18), a proinflammatory cytokine, leads to IFN‐γ production by NK or T cells, induces Th1 differentiation and suppresses IgE synthesis by B cells when acting on responding cells together with IL‐12. IL‐18 also exhibits biological activities related to allergic inflammation such as histamine or IL‐4 release from basophils and accumulation of eosinophils in localized lesions in allergic model mice. In this study, Reverse transcription (RT)‐PCR analysis revealed that IL‐18 receptor α chain mRNA was expressed in both freshly prepared eosinophils and two eosinophilic cell lines (YY‐1 and EoL‐1 cells). Flow cytometry and RT‐PCR analyses revealed that the treatment of YY‐1 cells with n‐butyric acid promoted cell maturation and caused an enhancement of IL‐18 receptor α chain expression. IL‐18 had little effect on the survival of peripheral eosinophils, but it dose‐dependently augmented IL‐8 synthesis by YY‐1 cells. In addition, IL‐18‐mediated up‐regulation of IL‐8 expression in eosinophils from a patient suffering from hyper‐eosinophilic syndrome was confirmed. Our findings using peripheral blood eosinophils and eosinophilic cell line suggest the functional importance of IL‐18 in the induction of IL‐8 and a potential proinflammatory role in allergy.

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Hl-60

Drexler

2001

The Leukemia-Lymphoma Cell Line FactsBook

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Effects of Eosinophilotropic Cytokines on Differentiation of an Eosinophil Cell Line, YY-1

Cited by 3 publications

References 2 publications

Eosinophils in Fungus-Associated Allergic Pulmonary Disease

Eosinophils in Fungus-Associated Allergic Pulmonary Disease

Interleukin-18 enhances the production of interleukin-8 by eosinophils

Hl-60

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