Effects of epoxy stearic acid on lipid metabolism in HepG2 cells

Liu, Ying; Li, Jinwei; Liu, Yuanfa

doi:10.1111/1750-3841.15405

Cited by 12 publications

(9 citation statements)

References 45 publications

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“…The second blow (oxidative stress response) is based on excessive accumulation of lipids, which produces a large amount of free radicals and reactive oxygen species. Extensive research has shown that a certain concentration of oleic acid (OA) can cause oxidative stress in HepG2 cells (Liu et al., 2020; Yu et al., 2019). After adipose and liver tissue induced by reactive oxygen species, inflammation occurs in the liver cells (Mantena et al., 2008).…”

Section: Introductionmentioning

confidence: 99%

Major royal jelly proteins prevents NAFLD by improving mitochondrial function and lipid accumulation through activating the AMPK / SIRT3 pathway in vitro

Zhang

Zhou

et al. 2021

Journal of Food Science

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Nonalcoholic fatty liver disease (NAFLD) is a metabolic syndrome, whose main characteristics are excessive lipid accumulation and oxidative stress. Major royal jelly proteins (MRJPs) is a kind of water‐soluble protein, which is abundant in royal jelly (RJ). The aim of this study was to evaluate the effect of MRJPs on lipid accumulation and oxidative stress of liver cells. Here, we first optimized the conditions for extracting MRJPs from RJ and identified the extraction effect and product by SDS‐PAGE. Then, we used oleic acid (OA) of 1.0 mM to induce hepatocytes for 24 hr to establish a stable cell models of lipid accumulation, and we found that pre‐administration (24 hr) of MRJPs (0.2, 0.5, and 1.0 g/L) could significantly reduce the lipid drop content and triglyceride level in the model cells, and simultaneously reduce the alanine aminotransferase and aspertate aminotransferase levels in the cell culture supernatant. In addition, pre‐incubation (24 hr) with MRJPs (0.2, 0.5, and 1.0 g/L) could restore superoxide dismutase (SOD) level and mitochondrial membrane potential as compared with OA group. Furthermore, MRJPs administration significantly upregulated the expression of Silent Information Regulator 2 Associated Protein 3, mitochondrial superoxide dismutase (SOD2), and cytochrome c oxidase subunit IV in OA‐treated HepG2 cells. The study for the first time provides evidences on the lipid‐lowering effect of MRJPs at the cellular level, which can further provide support for the development and application of polypeptide drugs in the future, and can also provide a choice for the prevention and treatment of liver metabolic diseases represented by NAFLD. Practical Application Our study proved that MRJPs had substantial preventing effect on OA‐induced lipid accumulation and mitochondrial dysfunction in HepG2 cells. This research can further provide theoretical support for the development and application of peptide drugs in the future. Besides, it can not only further broaden our understanding of NAFLD and other diseases, but also provide ideas for research on oxidative stress and lipid accumulation in the body.

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Section: Introductionmentioning

confidence: 99%

Major royal jelly proteins prevents NAFLD by improving mitochondrial function and lipid accumulation through activating the AMPK / SIRT3 pathway in vitro

Zhang

Zhou

et al. 2021

Journal of Food Science

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“…To the best of our knowledge, the biological activities of 12(13)-EpODE and 14(15)-EpEDE have not been investigated so far. High levels of 9(10)-epoxy-stearic acid may induce lipid accumulation and oxidative stress in HepG2 cells [ 74 , 75 ]. Among the vicinal dihydroxy-PUFAs that were substantially and significantly reduced in MetS participants, 9,10-DiHOME was shown to increase coronary resistance [ 76 ] and to attenuate insulin signaling [ 73 ] but no information is available for 7,8-DiHDPE.…”

Section: Discussionmentioning

confidence: 99%

The Plasma Oxylipin Signature Provides a Deep Phenotyping of Metabolic Syndrome Complementary to the Clinical Criteria

Dalle

Tournayre

Mainka

et al. 2022

IJMS

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Metabolic syndrome (MetS) is a complex condition encompassing a constellation of cardiometabolic abnormalities. Oxylipins are a superfamily of lipid mediators regulating many cardiometabolic functions. Plasma oxylipin signature could provide a new clinical tool to enhance the phenotyping of MetS pathophysiology. A high-throughput validated mass spectrometry method, allowing for the quantitative profiling of over 130 oxylipins, was applied to identify and validate the oxylipin signature of MetS in two independent nested case/control studies involving 476 participants. We identified an oxylipin signature of MetS (coined OxyScore), including 23 oxylipins and having high performances in classification and replicability (cross-validated AUCROC of 89%, 95% CI: 85–93% and 78%, 95% CI: 72–85% in the Discovery and Replication studies, respectively). Correlation analysis and comparison with a classification model incorporating the MetS criteria showed that the oxylipin signature brings consistent and complementary information to the clinical criteria. Being linked with the regulation of various biological processes, the candidate oxylipins provide an integrative phenotyping of MetS regarding the activation and/or negative feedback regulation of crucial molecular pathways. This may help identify patients at higher risk of cardiometabolic diseases. The oxylipin signature of patients with metabolic syndrome enhances MetS phenotyping and may ultimately help to better stratify the risk of cardiometabolic diseases.

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Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, the biological activities of 12(13)-EpODE and 14(15)-EpEDE have not been investigated so far. High levels of 9(10)-epoxy-stearic acid may induce lipid accumulation and oxidative stress in HepG2 cells (87, 88). Among the vicinal dihydroxy-PUFAs that were substantially and significantly reduced in MetS participants, 9,10-DiHOME was shown to increase coronary resistance (89) and to attenuate insulin signaling (86) but no information is available for 7,8-DiHDPE.…”

Section: Discussionmentioning

confidence: 99%

Oxylipin profiling identifies a mechanistic signature of metabolic syndrome: results from two independent cohorts

Dalle

Tournayre

Mainka

et al. 2022

Preprint

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Background. Metabolic syndrome (MetS) is a complex condition encompassing a constellation of cardiometabolic abnormalities. Integratively phenotyping the molecular pathways involved in MetS would help to deeply characterize its pathophysiology and to better stratify the risk of cardiometabolic diseases. Oxylipins are a superfamilly of lipid mediators regulating most biological processes involved in cardiometabolic health. Methods. A high-throughput validated mass spectrometry method allowing the quantitative profiling of over 130 oxylipins was applied to identify and validate the oxylipin signature of MetS in two independent case/control studies involving 476 participants. Results. We have uncovered and validated an oxylipin signature of MetS (coined OxyScore) including 23 oxylipins and having high performances of classification and replicability (cross-validated AUCROC of 89%, 95% CI: 85%-93% and 78%, 95% CI: 72%-85% in the Discovery and Replication studies, respectively). Correlation analysis and comparison with a classification model incorporating both the oxylipins and the MetS criteria showed that the oxylipin signature brings consistent and complementary information supporting its clinical utility. Moreover, the OxyScore provides a unique mechanistic signature of MetS regarding the activation and/or negative feedback regulation of crucial molecular pathways that may help identify patients at higher risk of cardiometabolic diseases. Conclusion. Oxylipin profiling identifies a mechanistic signature of metabolic syndrome that may help to enhance MetS phenotyping and ultimately to better predict the risk of cardiometabolic diseases via a better patient stratification.

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Effects of epoxy stearic acid on lipid metabolism in HepG2 cells

Cited by 12 publications

References 45 publications

Major royal jelly proteins prevents NAFLD by improving mitochondrial function and lipid accumulation through activating the AMPK / SIRT3 pathway in vitro

Major royal jelly proteins prevents NAFLD by improving mitochondrial function and lipid accumulation through activating the AMPK / SIRT3 pathway in vitro

The Plasma Oxylipin Signature Provides a Deep Phenotyping of Metabolic Syndrome Complementary to the Clinical Criteria

Oxylipin profiling identifies a mechanistic signature of metabolic syndrome: results from two independent cohorts

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