Effects of ergosterol, palmitic acid and related simple lipids on the recovery of Candida albicans from ultraviolet irradiation

Sarachek, Alvin; Higgins, N. Patrick

doi:10.1007/bf00424928

Cited by 27 publications

(15 citation statements)

References 22 publications

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“…The absorption and antifungal activity of polyene antibiotics are inhibited by the presence of fatty acids. Inhibition increases with fatty acid chain length and unsaturation (11,15,26). The presence of long-chain unsaturated fatty acids in the medium prevented a large proportion of added candicidin binding to the yeast.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the bulk of the bound candicidin is associated with an un-I Present address: University of Bristol, Long Ashton Research Station, Bristol, BS18 9AF, England. known component in the yeast cell wall in addition to the active site in the cell membrane (13). Addition of fatty acids to yeast suspensions prevents polyene antifungal activity (11,15,26). The lipid composition of the cell wall of C. albicans was investigated to see whether this could provide a possible explanation of the observed facts.…”

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confidence: 99%

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Mode of Action of the Polyene Antibiotic Candicidin: Binding Factors in the Wall of Candida albicans

Hammond

Kliger

1976

Antimicrob Agents Chemother

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The polyene antibiotic candicidin produces a rapid efflux of K + ions from a suspension of Candida albicans . Onset of K + leakage depends on the culture age, stationary-phase yeasts leaking K + more slowly than exponential-phase yeasts. The time taken for potassium leakage to begin represents the time taken by the antibiotic to cross the cell wall and produce membrane damage. It was shown that there were factors in the cell wall of C. albicans that increased their total binding capacity and their affinity for candicidin during growth. An attempt was made to relate changes in the lipid content of the yeast cell with the increased time taken to produce membrane damage.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Mode of Action of the Polyene Antibiotic Candicidin: Binding Factors in the Wall of Candida albicans

Hammond

Kliger

1976

Antimicrob Agents Chemother

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“…show enhanced survival following ultraviolet (uv) irradiation if grown at low temperature, in the presence of sterols or long chain fatty acids, or on minimal rather than enriched medium (Busbee and Sarachek, 1969;Sarachek and Higgins, 1972). Sarachek and Higgins (1972) have summarized evidence suggesting that each of these effects related to recovery from uv induced disturbance of organizational features of the cell membrane which are critical for the initiation of cell division.…”

Section: Non-budding Cells Of Candida Albicansmentioning

confidence: 99%

“…Sarachek and Higgins (1972) have summarized evidence suggesting that each of these effects related to recovery from uv induced disturbance of organizational features of the cell membrane which are critical for the initiation of cell division. Recently, Molzahn and Woods (1972) Tables 1 and 2 compare the growth rates and susceptibilities to inactivation by amphotericin B of WD-18-4 and the representative group of its antibiotic-resistant derivatives described in this report; the resistant strains WD-18-4(5A), WD-18-4(7A) and were obtained by selection at 25 C whereas strains WD-18-4(20B) and WD-18-4(25B) were obtained by selection at 37° C. Procedures for measuring growth rates and cellular inactivation by amphotericin B were those of Bish and Sarachek (1967) and of Sarachek and Higgins (1972). For both kinds of determinations, experimental flasks were incubated on reciprocal shakers operating at 80, 1.5 inch strokes per minute.…”

Section: Non-budding Cells Of Candida Albicansmentioning

confidence: 99%

Influences of cellular susceptibility to amphotericin B and of post- irradiation growth conditions on inactivaion of Candida albicans by ultraviolet radiation

Sarachek¹,

Pettriess²

1973

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“…Ghosh and Ghosh (2), investigating the effect of various lipids on the uptake of nystatin by Candida albicans, found that the uptake was antagonized more effectively by linoleic acid than by ergosterol, cholesterol, vitamin A, or oleic acid. Sarachek and Higgins (8) showed that fatty acids could protect nonirradiated cells from damage by amphotericin B and also significantly enhance the recovery of ultraviolet light-damaged cells. The protective action increased with chain length, but the unsaturated C18 acids, oleic, linoleic, and linolenic, were less effective than saturated C18 stearic acid.…”

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confidence: 99%

Effect of fatty acids on action of polyene antibiotics

Iannitelli¹,

Ikawa²

1980

Antimicrob Agents Chemother

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Fatty acids cause a decrease in the absorption spectra of the antifungal polyene macrolide antibiotics nystatin, filipin, candicidin, and amphotericin B. For nystatin, fi6pin, and candicidin, this decrease in absorption could be correlated with the activity of the fatty acids in protecting the yeast Saccharomyces cerevisiae against the action of these antibiotics. With amphotericin B a correlation was observed between the decrease in absorption caused by certain derivatives of fatty acids and the protective action of these derivatives against the activity of amphotericin B on yeast. It is concluded that, like the sterols, fatty acids also interact with the polyene antibiotics and thereby reduce their effective concentrations.The polyene macrolide antibiotics are effective antifungal agents and, as a rule, are inactive against bacteria. The polyenes interact with sterols of the cell membrane to alter its structure and cause the leakage of essential metabolites (see reference 3). Lampen et al. (6) showed that when ergosterol or cholesterol was incorporated into the culture medium, yeasts were protected from the action of polyenes such as filipin, nystatin, and antimycoin.Hickey (5) has shown that the action of the polyene ascosin on the yeast Saccharomyces cerevisiae was antagonized by fatty acids, the longer-chain-length acids and the more unsaturated acids being the most effective. Ghosh and Ghosh (2), investigating the effect of various lipids on the uptake of nystatin by Candida albicans, found that the uptake was antagonized more effectively by linoleic acid than by ergosterol, cholesterol, vitamin A, or oleic acid. Sarachek and Higgins (8) showed that fatty acids could protect nonirradiated cells from damage by amphotericin B and also significantly enhance the recovery of ultraviolet light-damaged cells. The protective action increased with chain length, but the unsaturated C18 acids, oleic, linoleic, and linolenic, were less effective than saturated C18 stearic acid. Recently, Hammond and Kliger (4) showed that resistance to membrane damage of C. albicans by candicidin increased with the age of the culture. This effect was correlated with an increase in cell wall triglycerides with age. Lampen et al. (6) studied the binding of the polyene antibiotics filipin, nystatin, and antimycoin to sterols by spectrophotometric means. They found that sterols significantly reduced the absorbance of the polyenes at their absorption maxima. This was interpreted to mean that the sterols decreased the effective concentration of the polyenes in the mixture. This was borne out by the fact that the addition of isopropyl alcohol restored the absorbance because of the resolubilization of the polyenes. Bittman and Fischkoff (1), by fluorescence studies, concluded that polyene-cholesterol binding did take place in an aqueous buffer and that the binding was accomplished by hydrophobic interactions.A study was made to determine whether changes in the absorbance of polyene antibiotics could be produced by fatty acids and whether t...

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Effects of ergosterol, palmitic acid and related simple lipids on the recovery of Candida albicans from ultraviolet irradiation

Cited by 27 publications

References 22 publications

Mode of Action of the Polyene Antibiotic Candicidin: Binding Factors in the Wall of Candida albicans

Mode of Action of the Polyene Antibiotic Candicidin: Binding Factors in the Wall of Candida albicans

Influences of cellular susceptibility to amphotericin B and of post- irradiation growth conditions on inactivaion of Candida albicans by ultraviolet radiation

Effect of fatty acids on action of polyene antibiotics

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