Effects of Erythrocyte Microvesicles on the Coagulation Process Stages

Abstract

We studied the effect of erythrocyte microvesicles on various stages of platelet-free autoplasma coagulation process. The erythrocyte microvesicles not only exhibited procoagulant activity, but also produced a pronounced anticoagulant effect by inhibiting fibrin formation process. The effects of microvesicles isolated from erythrocytes after 24- and 48-hour cell storage on plasma coagulation were different. The hemocoagulation effects of the "light" and "heavy" microvesicles were studied.

Red Blood Cell-Derived Extracellular Vesicles: An Overview of Current Research Progress, Challenges, and Opportunities

“…Moreover, the procoagulant activity of RBC EVs significantly increases during RCC storage [46]. The procoagulant activity of RBC EVs isolated from stored RCCs after 24 and 48 h storage was even different [47]. Although it is widely recognized that the storage of RBC is associated with impairment of their properties that can induce a circulatory risk to recipients, the specific mechanisms related to this phenomenon are still not fully understood currently.…”

Antithrombin Activity of Erythrocyte Microvesicles

“…This process becomes particularly important during erythrocyte storage, because it can underlie some post-transfusion complications in the recipients [10]. Previously we observed an increase in thrombin time under the effect of eMV [6], which prompted us to examine antithrombin activity (ATA) of eMV.Our aim was to determine the effect of erythrocyte preservation on ATA of eMV isolated from stored erythrocytes. …”