Effects of estradiol on aldosterone secretion in ovariectomized rats

Kau, Mei-Mei; Lo, Ming-Jae; Tsai, Shiow-Chwen; Chen, Jiann‐Jong; Lu, Chunliang; H, Lin; Wang, Shyi-Wu; Wang, Paulus S.

doi:10.1002/(sici)1097-4644(19990401)73:1<137::aid-jcb15>3.0.co;2-7

Cited by 17 publications

(12 citation statements)

References 32 publications

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“…However, these findings are in contrast to published studies on animal models, where progesterone increased aldosterone production by ASWT [17] and increased mRNA CYP11B2 production [17]. In our system, estradiol showed no effect on both ASCE and ASWT activities in contrast to the results reported by Kau, where an increase in the production of aldosterone by estradiol replacement in ovariectomised rat was shown [43]. …”

Section: Discussioncontrasting

confidence: 99%

Different effects of progesterone and estradiol on chimeric and wild type aldosterone synthase in vitro

Vecchiola

Lagos

Fuentes

et al. 2013

Reprod Biol Endocrinol

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BackgroundFamilial hyperaldosteronism type I (FH-I) is caused by the unequal recombination between the 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes, resulting in the generation of a CYP11B1/B2 chimeric gene and abnormal adrenal aldosterone production. Affected patients usually show severe hypertension and an elevated frequency of stroke at a young age. Aldosterone levels rise during pregnancy, yet in pregnant women with FH-1, their hypertensive condition either remains unchanged or may even improve. The purpose of this study was to investigate in vitro whether female sex steroids modulate the activity of chimeric (ASCE) or wild type (ASWT) aldosterone synthase enzymes.MethodsWe designed an in vitro assay using HEK-293 cell line transiently transfected with vectors containing the full ASCE or ASWT cDNAs. Progesterone or estradiol effects on AS enzyme activities were evaluated in transfected cells incubated with deoxycorticosterone (DOC) alone or DOC plus increasing doses of these steroids.ResultsIn our in vitro model, both enzymes showed similar apparent kinetic parameters (Km = 1.191 microM and Vmax = 27.08 microM/24 h for ASCE and Km = 1.163 microM and Vmax = 36.98 microM/24 h for ASWT; p = ns, Mann–Whitney test). Progesterone inhibited aldosterone production by ASCE- and ASWT-transfected cells, while estradiol demonstrated no effect. Progesterone acted as a competitive inhibitor for both enzymes. Molecular modelling studies and binding affinity estimations indicate that progesterone might bind to the substrate site in both ASCE and ASWT, supporting the idea that this steroid could regulate these enzymatic activities and contribute to the decay of aldosterone synthase activity in chimeric gene-positive patients.ConclusionsOur results show an inhibitory action of progesterone in the aldosterone synthesis by chimeric or wild type aldosterone synthase enzymes. This is a novel regulatory mechanism of progesterone action, which could be involved in protecting pregnant women with FH-1 against hypertension. In vitro, both enzymes showed comparable kinetic parameters, but ASWT was more strongly inhibited than ASCE. This study implicates a new role for progesterone in the regulation of aldosterone levels that could contribute, along with other factors, to the maintenance of an adequate aldosterone-progesterone balance in pregnancy.

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Section: Discussioncontrasting

confidence: 99%

Different effects of progesterone and estradiol on chimeric and wild type aldosterone synthase in vitro

Vecchiola

Lagos

Fuentes

et al. 2013

Reprod Biol Endocrinol

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“…However, in this study, both pregnant and nonpregnant women were in 100 mEq sodium balance. Nonetheless, other factors that are elevated in pregnancy may increase aldosterone synthase activity, such as estradiol (34).…”

Section: Discussionmentioning

confidence: 99%

The Renin-Aldosterone Response to Stimulation and Suppression During Normal Pregnancy

Bentley‐Lewis¹,

Graves²,

Seely³

2005

LHIP

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Objective-During normal pregnancy, studies have shown increased activity of the reninangiotensin-aldosterone system (RAAS) and a dissociation of plasma renin activity (PRA) and aldosterone (Aldo) evidenced by a greater increase in Aldo relative to PRA. The aims of this study were to examine the RAAS response to stimulation by upright posture and suppression by saline infusion and to investigate the PRA-Aldo dissociation under these two conditions. Methods-We studied 24 healthy normotensive women (mean ± standard error of mean, ages 29 ± 1 yrs) in sodium (Na) balance in the second and third trimesters and postpartum. Subjects underwent a 24-hour urine collection which was analyzed for Na, norepinephrine (NE), epinephrine (Epi), and dopamine (DA); a posture study with analysis of blood pressure (BP), PRA, Aldo, NE, Epi, DA, and cortisol; and a 0.9% NaCl infusion study (500 mL/hr for 3 hrs) with analysis of BP, PRA, Aldo, cortisol, and digitalis-like factor (DLF). Analyses included paired t tests to compare posture and saline responses, repeated measures to compare across periods, and percent change to evaluate the PRA-Aldo dissociation.Results-During pregnancy, PRA, Aldo, BP, catecholamines, and cortisol levels were significantly greater in upright than left lateral decubitus (LLD) posture, and the percent change in Aldo was significantly greater than the percent change in PRA. During pregnancy in response to saline infusion, BP did not change; the PRA and Aldo significantly decreased; the percent change in Aldo was significantly greater than the percent change in PRA in the second trimester; and serum DLF and cortisol levels significantly decreased.Conclusions-In longitudinally studied normal pregnancy, PRA and Aldo levels were dissociated at baseline, with stimulation and, to a lesser degree, with suppression. Norepinephrine, adrenocorticotrophic hormone, and DLF may contribute to this dissociation, and clarification of these interactions may provide insight into the regulation of aldosterone during normal and hypertensive pregnancy.

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“…Nowak et al (1995) indicated the stimulatory effect of oestradiol on basal corticosterone release in rats in vitro. Priming of ovariectomised rats with oestradiol benzoate causes an increase of basal and ACTHstimulated corticosterone (Lo et al, 2000) and aldosterone (Kau et al, 1999) release in vitro. On the other hand, Mesiano et al (1999) reported that oestradiol suppressed cAMP-induced cortisol secretion by H295 cells, whilst both basal and stimulated production of DHEA was enhanced.…”

Section: Discussionmentioning

confidence: 96%

Phytoestrogens alter cortisol and and rostenedione secretion by porcine adrenocortical cells

Kamińska

Opałka

Dusza

2007

Acta Veterinaria Hungarica

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The effect of plant-derived, nonsteroidal estrogens (genistein, daidzein and biochanin A) on cortisol and androstenedione secretion by porcine adrenocortical cells was examined. Adrenals were harvested from locally slaughtered mature gilts on days 5-9 of the oestrous cycle. Adrenocortical cells were incubated with or without genistein, daidzein, biochanin A (0.5, 5, 10 or 50 µM), oestradiol (10, 50, 100 or 500 pg/mL) and ACTH (5 nM -positive control). All incubations were performed for 8 h (95% air and 5% CO 2 , 37 °C). ACTH increased cortisol and androstenedione secretion. Genistein, daidzein and biochanin A suppressed cortisol output, whereas androstenedione secretion was enhanced by these phytoestrogens. In contrast, oestradiol did not alter steroid secretion by porcine adrenocortical cells, which may suggest that phytoestrogens have a non-oestrogenic mechanism of action. Therefore, phytoestrogens present in commercial forage may influence adrenocortical function in pigs by decreasing cortisol and increasing androstenedione secretion. Key words: Phytoestrogens, isoflavones, adrenocortical cells, pigPhytoestrogens are phytochemicals present in whole plants, their seeds and plant-derived preserves (Reinli and Block, 1996). These compounds are widely present in legumes and beans, which are the main components of the fodder of farm animals and constitute an integral part of the human diet (Lundh, 1995;Duncan et al., 2003). Soybeans, commonly used in pig diets, are the most significant source of isoflavones (one of the phytoestrogen classes) including

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Effects of estradiol on aldosterone secretion in ovariectomized rats

Cited by 17 publications

References 32 publications

Different effects of progesterone and estradiol on chimeric and wild type aldosterone synthase in vitro

Different effects of progesterone and estradiol on chimeric and wild type aldosterone synthase in vitro

The Renin-Aldosterone Response to Stimulation and Suppression During Normal Pregnancy

Phytoestrogens alter cortisol and and rostenedione secretion by porcine adrenocortical cells

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