Effects of Estrogen Receptor Alpha on Vascular Smooth Muscle Cell SM22

Abstract

Abstract-Myocardin and associated transcription factor (Myocardin-Related Transcription Factors, MRTFs) by serum response factor (SRF) together constitute the composition of molecular differentiation of muscle cell switch. This paper focuses on the vascular smooth muscle cells (vascular smooth muscle cells, VSMCs), breast cancer cell lines MCF-7 and African green monkey kidney cell line COS-7 as the research object, using cellular and molecular biological methods to study the transcription factor Myocardin and Estrogen receptor α (ER α) in the regulation of differentiation of vascular smooth muscle cell function. The luciferase reporter assay in COS-7 and MCF-7 cell lines and VSMCs (Luciferase Report assay) method to study the ER alpha for Myocardin differentiation ability, the results showed that ER α can inhibit SM22 α Myocardin for transcription activation. At the same time, the expression of estradiol and tamoxifen treatment by transfection group for detection of SM22 and found that blocking the ER alpha for the inhibitory effect of Myocardin differentiation ability can be ER alpha antagonist tamoxi.