Effects of estrogen via estrogen receptors on parvalbumin levels in cardiac myocytes of ovariectomized rats

Wirakiat, Wimon; Udomuksorn, Wandee; Vongvatcharanon, Surapong; Vongvatcharanon, Uraporn

doi:10.1016/j.acthis.2011.02.004

Cited by 8 publications

(4 citation statements)

References 29 publications

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“…In the latter group, interestingly, this finding is further supported by the upregulation of molecular pathways involved in PV expression, such as Pgc-1 α, required for both mRNA and protein expression of PV in the hippocampus ( Lucas et al, 2010 ; Jinde et al, 2013 ) and a master regulator of mitochondrial biogenesis ( St-Pierre et al, 2006 ), and Sirt 1 , a histone deacetylase that directly regulates the activity of PGC-1α ( Rodgers et al, 2008 ; Aquilano et al, 2010 ). This is not surprising, since PV-expressing interneurons are known to express estrogen receptors ( Blurton-Jones and Tuszynski, 2002 ; Higaki et al, 2012 ) and changes in size of the PV-expressing subpopulation following E2 treatment have previously been reported in different brain regions ( Rewal et al, 2005 ; Macrì et al, 2010 ; Sotonyi et al, 2010 ; Koh, 2014 ), as well as in non-neural tissues ( Wirakiat et al, 2012 ).…”

Section: Discussionmentioning

confidence: 73%

Estrogen administration modulates hippocampal GABAergic subpopulations in the hippocampus of trimethyltin-treated rats

Corvino

Maria

Marchese

et al. 2015

Front. Cell. Neurosci.

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Given the well-documented involvement of estrogens in the modulation of hippocampal functions in both physiological and pathological conditions, the present study investigates the effects of 17-beta estradiol (E2) administration in the rat model of hippocampal neurodegeneration induced by trimethyltin (TMT) administration (8 mg/kg), characterized by loss of pyramidal neurons in CA1, CA3/hilus hippocampal subfields, associated with astroglial and microglial activation, seizures and cognitive impairment. After TMT/saline treatment, ovariectomized animals received two doses of E2 (0.2 mg/kg intra-peritoneal) or vehicle, and were sacrificed 48 h or 7 days after TMT-treatment. Our results indicate that in TMT-treated animals E2 administration induces the early (48 h) upregulation of genes involved in neuroprotection and synaptogenesis, namely Bcl2, trkB, cadherin 2 and cyclin-dependent-kinase-5. Increased expression levels of glutamic acid decarboxylase (gad) 67, neuropeptide Y (Npy), parvalbumin, Pgc-1α and Sirtuin 1 genes, the latter involved in parvalbumin (PV) synthesis, were also evident. Unbiased stereology performed on rats sacrificed 7 days after TMT treatment showed that although E2 does not significantly influence the extent of TMT-induced neuronal death, significantly enhances the TMT-induced modulation of GABAergic interneuron population size in selected hippocampal subfields. In particular, E2 administration causes, in TMT-treated rats, a significant increase in the number of GAD67-expressing interneurons in CA1 stratum oriens, CA3 pyramidal layer, hilus and dentate gyrus, accompanied by a parallel increase in NPY-expressing cells, essentially in the same regions, and of PV-positive cells in CA1 pyramidal layer. The present results add information concerning the role of in vivo E2 administration on mechanisms involved in cellular plasticity in the adult brain.

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Section: Discussionmentioning

confidence: 73%

Estrogen administration modulates hippocampal GABAergic subpopulations in the hippocampus of trimethyltin-treated rats

Corvino

Maria

Marchese

et al. 2015

Front. Cell. Neurosci.

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“…Based on the time course of previous studies investigating neuromuscular adaptations to surgically induced menopause, rats were euthanized after 10 weeks. 14,18 For control animals, the estrous cycle was not controlled for on the day of euthanization. After euthanization, larynges were excised, bisected, fixed in 4% formaldehyde for 1 hour, and cryoprotected overnight in 20% sucrose solution at 4 C. Hemilarynges were then flash frozen in isopentane cooled to just above freezing temperature and stored at −80 C until processed.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the time course of previous studies investigating neuromuscular adaptations to surgically induced menopause, rats were euthanized after 10 weeks 14,18 . For control animals, the estrous cycle was not controlled for on the day of euthanization.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds

Lenell

Johnson

2020

The Laryngoscope

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Objectives/Hypothesis: Menopause adversely affecs power and endurance of the limb muscles. However, despite clinical observations that menopause corresponds to negative changes of the voice, the direct effects of estrogen deprivation on the thyroarytenoid muscles are unknown. The purpose of this study was to investigate the effects of estrogen deprivation via ovariectomy on three neuromuscular parameters of the thyroarytenoid muscles using a rat model.Study Design: Animal model. Methods: Cryosections of vocal folds of 20 (10 control and 10 ovariectomized) female rats were stained to label neuromuscular junctions, fiber size, or parvalbumin levels using immunohistochemical techniques and compared between experimental groups.Results: The neuromuscular junctions, thyroarytenoid fiber sizes, and parvalbumin levels of the vocal folds were similar between experimental groups.Conclusions: The loss of estrogen did not change neuromuscular parameters of the vocal folds of adult female rats; therefore, vocal changes within the outer vibratory layers of the vocal folds may primarily be responsible for clinically observed menopausal vocal changes.

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“…The collected epithelial cells were stained with 0.5% methylene blue. The state of evaluation of the estrous cycle was determined according to the work of Wirakiat et al (2012). The rats were in the stage of diestrus before ovariectomy, except for the SHAM group.…”

Section: Evaluation Of the Estrous Cyclementioning

confidence: 99%

Treatment with Pueraria mirifica Extract Prevented Muscle Atrophy and Restored Muscle Strength in Ovariectomized Rats

Inthanuchit¹,

Udomuksorn²,

Kumarnsit³

et al. 2017

JSM

Self Cite

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Pueraria mirifica (PM) is a phytoestrogen-rich plant that was tested to establish if its phytosteroids could prevent estrogen dependent sarcopenia. The effect of PM on the estrogen levels, estrous cycle, toxicity, muscle mass, strength and endurance of extensor digitorum longus (EDL) and gastrocnemius muscles of ovariectomized rats was investigated. Adult female Wistar rats were divided into six groups: Sham-operated (SHAM); ovariectomized (OVX) fed with distilled water (PM0); OVX injected with 40 µg/kg estradiol benzoate (E40); (4-6) OVX fed with ethanolic extract of PM at doses of 50 (PM50), 500 (PM500) and 1000 (PM1000) mg/kg for 90 days. After treatment with all three doses of PM, no toxicity was detected to the hematopoietic system and liver function whereas the E40 group did show toxic effects. Treatment with 50 and 500 mg/kg of PM showed no effect on uterine hypertrophy and caused no arrest of the estrous cycle whereas treatment with estrogen and 1000 mg/kg of PM treatment did. The estrogen level, the cross sectional area of the EDL and the gastrocnemius muscle fiber strength and endurance were all significantly reduced in the PM0 group compared to that of the SHAM group (p<0.05) but were significantly increased in the E40, PM50, PM500 and PM1000 compared to that of the PM0 group (p<0.05). This indicated that the estrogenic activity of PM alleviated muscle atrophy and built up muscle strength and endurance. Thus, the 50 and 500 mg/kg of PM were suitable for treating estrogen dependent sarcopenia in ovariectomized rats.

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Effects of estrogen via estrogen receptors on parvalbumin levels in cardiac myocytes of ovariectomized rats

Cited by 8 publications

References 29 publications

Estrogen administration modulates hippocampal GABAergic subpopulations in the hippocampus of trimethyltin-treated rats

Estrogen administration modulates hippocampal GABAergic subpopulations in the hippocampus of trimethyltin-treated rats

The Effects of Menopause on Neuromuscular Parameters of the Rat Vocal Folds

Treatment with Pueraria mirifica Extract Prevented Muscle Atrophy and Restored Muscle Strength in Ovariectomized Rats

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