Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages

Blonska, Marzenna; Bronikowska, Joanna; Pietsz, Grazyna; Czuba, Zenon; Scheller, S; Król, Wojciech

doi:10.1016/j.jep.2003.11.011

Cited by 116 publications

(98 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stimulation of immune system by natural products has already been reported (Hegazi et al, 1995;Kong et al, 2004). Not only in broilers, but also in rodents these effect of propolis has been confirmed (Blonska et al, 2004;Giurgea et al, 1983). The effect of natural products such as propolis on immune system of different species is interesting and complicated.…”

Section: Resultsmentioning

confidence: 87%

Humoral Immunity of Broilers is Affected by Oil Extracted Propolis (OEP) in the Diet

Rahmani

Pourreza²

2005

International J. of Poultry Science

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 87%

Humoral Immunity of Broilers is Affected by Oil Extracted Propolis (OEP) in the Diet

Rahmani

Pourreza²

2005

International J. of Poultry Science

View full text Add to dashboard Cite

show abstract

“…In human HT1080 fibrosarcoma cells CAPE also decrease mRNA expression of matrix metalloproteinase (MMP) that plays a pivotal role in tumor metastasis by cleavage of extracellular matrix (35). Chrysin has been shown to exert anti-inflammatory and anti-carcinogenic effects through the inhibition of mRNA expression of interleukin-1ß (IL-1ß), inducible nitric oxide synthase (iNOS), and COX-2 (36,37). Artepillin C derived from Brazilian propolis caused a dose-dependent decrease in growth of CaCc2 human colon carcinoma cells, and it arrested the cells in G0/G1 phase via a stimulation of p21 CIP1 mRNA expression (38).…”

Section: Discussionmentioning

confidence: 99%

Growth inhibitory activity of ethanol extracts of Chinese and Brazilian propolis in four human colon carcinoma cell lines

Ishihara

Naoi²,

Hashita³

et al. 2009

Oncol Rep

View full text Add to dashboard Cite

Abstract. More than 300 bio-active compounds have been identified from bee propolis in various regions of the world. The objective of this study was to examine whether the ethanol extracts of Chinese and Brazilian propolis may exert anticancer activities in four human colon carcinoma cell lines, namely CaCo2, HCT116, HT29 and SW480. Propolis samples were extracted with ethanol, and the crude extracts were dissolved in dimethylsulfoxide and used for the experiments. In HCT116, HT29 and SW480 cell lines, the extracts of both Chinese and Brazilian propolis caused a marked dose-dependent growth inhibition, with IC 50 values in the range of 4-41 μg/ml. In HCT116 cell line, Chinese propolis extract induced apoptosis in the cells after 72 h of treatment. In addition, Chinese propolis extract caused a dose-dependent increase in the cellular mRNA levels of p21 CIP1 and p53 in the HCT116 cell line. These findings indicate that the ethanol extracts of propolis contain components that may have anticancer activity. Thus, propolis and related products may provide a novel approach to the chemoprevention and treatment of human colon carcinoma. IntroductionThere is growing interest in the use of natural products to aid in the maintenance of human health. Natural products contain a wide variety of chemical compounds that have potent biological effects, including anticancer activity (1). Identification of the active components and their mechanism of action is important to assess their potential for clinical use and possible diverse side effects. Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries in Europe, North and South America, China and Japan (2). Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties (2,3). Propolis from Europe, North and South America, Asia and Africa differ in their composition because of the differences in the local vegetation (2,3). The medical application of propolis has led to increased interest in its chemical compositions and its botanical origins since polyphenol compounds and their derivatives have been identified in propolis (2,3).Colon carcinoma is a common malignancy ranking third in frequency on a world wide basis and causes about 500,000 deaths annually (4). This malignancy is most common in developed countries of Western world, and mortality rate of this disease is rising (4). It is thought that one-third of human carcinoma might be associated with dietary habits and lifestyle (5) and dietary factors are known to be critical modulators of the development of human colon carcinoma (6,7). Despite recent clinical advances, colon carcinoma still remains a major issue because of its incidence, morbidity, and mortality (4,8,9). One promising approach to reduce the incidence and improve the prognosis of this malignancy may be chemoprevention (1,10).Although the above-described fi...

show abstract

“…The cell suspension (500 µl) was then incubated with 5 µl of annexin V-FITC and 10 µl of propidium iodide (PI) for 10 min at room temperature in the dark. The population of annexin V-positive cells was evaluated by flow cytometry (BD FAcScan, Becton-Dickinson Immnunocytometry Systems, San Jose, cA, USA) (11,13,25,31,32).…”

Section: Determination Of the Brazilian Green Propolis Profile By Thementioning

confidence: 99%

“…Propolis cannot be used as a raw material, so it must be purified by extraction to remove the inert material and preserve the polyphenolic fraction (20). The ethanolic extract of propolis (EEP) has attracted the interest of researchers in the last decade because of its many biological properties, such as antioxidant, immunomodulatory, chemopreventive and anticancer effects (11,(20)(21)(22)(23)(24)(25)(26)(27)(28)(29). These data suggest that propolis regulates multiple signalling pathways and has the potential to improve health and prevent diseases.…”

Section: Introductionmentioning

confidence: 99%

Ethanolic extract of Brazilian green propolis sensitizes prostate cancer cells to TRAIL-induced apoptosis

Szliszka

Zydowicz²,

Janoszka³

et al. 2011

Int J Oncol

View full text Add to dashboard Cite

Abstract. Prostate cancer represents an ideal disease for chemopreventive intervention. Propolis possesses immunomodulatory, anti-tumour and chemopreventive properties. The tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is an important endogenous anti-cancer agent that induces apoptosis selectively in tumour cells. However, some cancer cells are resistant to TRAIL-mediated apoptosis. Naturally occurring phenolic and polyphenolic compounds sensitize TRAIL-resistant cancer cells and augment the apoptotic activity of TRAIL. The ethanolic extract of Brazilian green propolis (EEP) is rich in phenolic components. Our in vitro results indicate the potential targets in the TRAILinduced apoptotic pathway for the cancer chemopreventive activity of Brazilian propolis. We examined the cytotoxic and apoptotic effects of Brazilian EEP and its bioactive components in combination with TRAIL on LNCaP prostate cancer cells. The chemical composition of Brazilian green propolis was determined by high performance liquid chromatographydiode array detection. The cytotoxicity was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl-tetrazolium and lactate dehydrogenase assays. Apoptosis was detected using annexin V-FITC by f low cytometry and f luorescence microscopy. The mitochondrial membrane potential (∆Ψm) was evaluated using DePsipher staining by fluorescence microscopy. Flow cytometry was used to analyse death receptor (TRAIL-R1 and TRAIL-R2) expression in LNCaP cells. The inhibition of nuclear factor-κB (NF-κB) (p65) activation in cancer cells was confirmed by the ELISA-based TransAM NF-κB kit. The LNCaP cells were shown to be resistant to TRAIL-induced apoptosis. Our study demonstrates that EEP sensitizes TRAIL-resistant prostate cancer cells. The main phenolic components detected in Brazilian green propolis are artepillin C, quercetin, kaempferol and p-coumaric acid. Brazilian propolis and its bioactive components markedly augmented TRAIL-mediated apoptosis and cytotoxicity in prostate cancer cells. Brazilian EEP enhanced the expression of TRAIL-R2 and the activity of NF-κB in LNCaP cells. The co-treatment of prostate cancer cells with 100 ng/ml TRAIL and 50 µg/ml EEP increased the percentage of apoptotic cells to 65.8±1.2% and caused a significant disruption of ∆Ψm in LNCaP cells. We show that Brazilian EEP helped cells overcome TRAIL resistance by engaging both intrinsic and extrinsic apoptotic pathways and regulating NF-κB activity.

show abstract

Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages

Cited by 116 publications

References 35 publications

Humoral Immunity of Broilers is Affected by Oil Extracted Propolis (OEP) in the Diet

Humoral Immunity of Broilers is Affected by Oil Extracted Propolis (OEP) in the Diet

Growth inhibitory activity of ethanol extracts of Chinese and Brazilian propolis in four human colon carcinoma cell lines

Ethanolic extract of Brazilian green propolis sensitizes prostate cancer cells to TRAIL-induced apoptosis

Contact Info

Product

Resources

About