Effects of ethanol, molasses and Lactobacillus plantarum on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages in the Tibetan plateau of China

Abstract

In Tibet, it is common practice to make and relocate total mixed ration (TMR) silages before feeding due to the uneven distribution of forages temporally and spatially. This study was conducted to investigate the effects of Lactobacillus plantarum (L), molasses (M) or ethanol (E) on the fermentation quality and aerobic stability of local adaptive TMR silage. After 45 days of ensiling, pH and ammonia nitrogen in inoculated TMR silages were significantly lower than control. During the first 6 days of the aerobic exposure test, a small fluctuation in lactic acid concentration for all TMR silages was observed, and then silages with ethanol continued this trend, while lactic acid in silage without ethanol sharply decreased until the end of the aerobic exposure period. Meanwhile, pH gradually increased along the aerobic exposure; silages treated with ethanol showed lower pH after 9 days of aerobic exposure. The population of yeast gradually increased during 6 days of aerobic exposure, after that an accelerated rise was observed in TMR silages without ethanol. The combinational beneficial effect of L. plantarum and ethanol was found in combined addition of ethanol and Lactobacillus plantarum silages (EL), indicated by intermediate fermentation quality and higher aerobic stability.

Effects of the Application of Lactobacillus plantarum Inoculant and Potassium Sorbate on the Fermentation Quality, In Vitro Digestibility and Aerobic Stability of Total Mixed Ration Silage Based on Alfalfa Silage

“…It was not conclusive as to whether the silage could directly stabilize the fermentation of TMR silage when the single silage composition accounted for more than half of the dry matter of the ingredients. Lactobacillus plantarum (LP) has been added to TMR silage and has proven to be effective in altering fermentation characteristics [8,9]. However, few studies have investigated whether it is necessary to reinoculate LP for TMR silage based on a large percentage of other silage ingredients, as well-fermented silage might already play the role of inoculant.…”

“…Furthermore, the main factor limiting the use of TMR silage by ruminants is the digestibility of the fiber. In an early study, LP improved the in vitro dry matter digestibility (DMD) of TMR silages [9]. Thus, an inoculant of LP was applied in alfalfa silage-based TMR silage to estimate the improvement of digestibility and to verify the necessity of inoculation.…”

A Combination of Novel Microecological Agents and Molasses Role in Digestibility and Fermentation of Rice Straw by Facilitating the Ruminal Microbial Colonization

“…An automatic trace gas recording system (AGRS) was used to measure the in vitro gas production, as described in our previous study (Yuan et al, 2016a ). Briefly, 500 mg of rice straw (6 replicates) were placed in 120 ml glass bottles.…”

Partial substitution of whole‐crop corn with bamboo shoot shell improves aerobic stability of total mixed ration silage without affecting in vitro digestibility

“…As a result, TMR silage is successfully developed in Japan (Imai, 2001) and popularized worldwide. Fermented TMR technology not only provides long‐term storage of TMR but also gives the possibility for exploring more agricultural by‐products as roughages (Yuan et al., 2016).…”

“…As a result, TMR silage is successfully developed in Japan (Imai, 2001) and popularized worldwide. Fermented TMR technology not only provides long-term storage of TMR but also gives the possibility for exploring more agricultural by-products as roughages (Yuan et al, 2016).Bamboo shoot as tasty food is widely consumed in many parts of the world, especially in China, Japan, the United States, India and Australia (Jiang et al, 2019). China is the largest producer and consumer of bamboo shoots, with an estimate production of…”