Effects of ethanol on antioxidant capacity in isolated rat hepatocytes

Abstract

AIM:To investigate dose-response and time-course of the effects of ethanol on the cell viability and antioxidant capacity in isolated rat hepatocytes. METHODS:Hepatocytes were isolated from male adult Wistar rats and seeded into 100-mm dishes. Hepatocytes were treated with ethanol at concentrations between 0 (C), 10 (E10), 50 (E50), and 100 (E100) mmol/L (dose response) for 12, 24, and 36 h (time course). Then, lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) concentration, glutathione (GSH) level, and activities of glutathione peroxidase (GPX), glutathione reductase (GRD), superoxide dismutase (SOD), and catalase (CAT) were measured. RESULTS:Our data revealed that LDH leakage was significantly increased by about 30% in group E100 over those in groups C and E10 at 24 and 36 h, The MDA concentration in groups C, E10 and E50 were significantly lower than that in group E100 at 36 h. Furthermore, the concentration of MDA in group E100 at 36 h was significantly higher by 4.5-and 1.7-fold, respectively, than that at 12 and 24 h. On the other hand, the GSH level in group E100 at 24 and 36 h was significantly decreased, by 32% and 28%, respectively, compared to that at 12 h. The activities of GRD and CAT in group E100 at 36 h were significantly less than those in groups C and E10. However, The GPX and SOD activities showed no significant change in each group. CONCLUSION:These results suggest that longtime incubation with higher concentration of ethanol (100 mmol/L) decreased the cell viability by means of reducing GRD and CAT activities and increasing lipid peroxidation.

Ameliorative Efficacy of Phytochemical Content of Corchorus olitorius Leaves against Acute Ethanol-induced Oxidative Stress in Wistar Rats

“…C. olitorius was able to reduce the ROS generation with subsequent decrease in GPX activity due to its high phytochemical content as reported by Orieke et al [54]. The increased activity of GPX observed in ethanol induced animals contradicts the studies of Airaodion et al [29] who observed no significant difference in the activity of GPX in the study of hepatoprotective effect of Parkia biglobosa on acute ethanol-induced oxidative stress in Wistar rats and that of Yang et al [56] who also observed no significant difference in GPX activities in rats hepatocyte exposed to varying concentrations of ethanol at an incubation time of 12 hours. The toxicity of ethanol is related to the product of its metabolic oxidation.…”

Diallyl sulfide protects against N -nitrosodiethylamineinduced liver tumorigenesis: Role of aldose reductase

“…As shown in the present study, NDEA produced a significant increase in hepatic MDA and NO levels, along with significant decreases in SOD and CAT activities. MDA was one of the main lipid peroxidation products; its elevated levels can reflect the degree of lipid-peroxidation-induced injury in hepatocytes [46] . On the other hand, it has been reported that SOD and CAT constitute a mutually supportive defense against ROS [47] .…”

Pathogenesis and management of alcoholic liver cirrhosis: a review

“…Oxidative stress, hepatocytes injury by ROS, is a major determinant in alcoholic liver injury and fibrosis 11,12. Production of ROS results in reduced antioxidant glutathione/glutathione disulfide ratio 13,14. On liver histology, hepatocyte injury is most significant in pericentral regions, where pericentral fibrosis occurs.…”