Effects of Exercise Training and Exhaustion on 45ca2+ Uptake and Ca-Atpase Activity of Rat Skeletal Muscle Microsomal and Mitochondrial Fractions

Bonner, Hugh W.; Leslie, Steven W.; Combs, Alan B.; Tate, Charlotte A.

doi:10.1249/00005768-197621000-00131

Cited by 7 publications

(9 citation statements)

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“…The only study of Bonner et al (1976) showed that endurance-trained rats displayed a higher SR Ca 2+ -ATPase activity at exhaustion than untrained rats. Surprisingly enough, inconsistent with previous observations in numerous studies (Byrd et al 1989;Yasuda et al 1999), they found higher SR Ca 2+ -ATPase activity after exercise than at rest.…”

Section: Introductionmentioning

confidence: 99%

“…The uncertainty in SR responses to training and acute exercise may have been, in part, confounded by earlier methodological approaches. Early studies on training effects, for example, utilized Millipore filtration procedure in which Ca 2+ transport is determined from the time-dependent uptake of 45 Ca 2+ into vesicles (Belcastro 1987;Bonner et al 1976;Kim et al 1981). The sensitivity of this approach is limited by the speed with which membrane vesicles can be separated and by the non-specific binding of radionuclides.…”

Section: Introductionmentioning

confidence: 99%

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Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles

et al. 2003

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Following 10 weeks of endurance training and in age-matched sedentary rats, sarcoplasmic reticulum (SR) Ca(2+)-uptake, Ca(2+)-release, and Ca(2+)-stimulated adenosinetriphosphatase (ATPase) activity were examined in homogenates of the plantaris and soleus muscles from rats subjected to moderate-intensity treadmill running to exhaustion. In order to examine the effects of acute exercise and/or training on SR Ca(2+)-handling capacity, comparisons between exhausted and non-exercised rats and between trained and untrained rats were performed. Our data confirm that Ca(2+)-sequestration by the SR from fast-twitch muscles is depressed after training. Immediately after exhaustive running, decreases in SR function occurred in both muscles, but were more pronounced in the soleus. In the plantaris, reductions in SR Ca(2+)-uptake rate and Ca(2+)-ATPase activity were observed in untrained rats only, while in the soleus they were adversely affected irrespective of training status. Although the average run time to exhaustion varied markedly between untrained and trained animals (untrained: 253.0 min; trained: 559.4 min), no differences existed with regard to the magnitude of decreases in SR function in the soleus after exercise. The mean rate of decline in SR Ca(2+)-handling capacity during acute exercise, as estimated from the run time and the extent of the decline, was more than twofold higher in untrained than in trained soleus. From the present study, it is unclear whether there exists a causal relationship between muscular fatigue and SR function because the run time to exhaustion was not significantly correlated with any of parameters indicative of SR Ca(2+)-handling capacity, but suggested that endurance training may be capable of delaying a progression of the deterioration in SR function that occurs during exercise.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles

et al. 2003

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“…Following endurance training of rats the Ca 2+ uptake of isolated SR was reduced, but not so in muscle homogenates (SEMBROWICH et al, 1978). Training had no significant effect on SR Ca 2+ uptake either at rest or following exercise (BONNER et al, 1976). There was hardly any significant effect of exercise on SR Ca 2+ ATPase, except for the line DU-hOF with an increase in activity by 29.3 % following an exhaustive run (Fig.…”

Section: Discussionmentioning

confidence: 84%

“…Exercise training increases physical fitness (MADSEN et al, 1994), but the effect on the SR Ca 2+ transport is ambiguous. In trained and non trained rats there was no difference in SR Ca 2+ uptake either at rest or after exhaustive exercise, but the Ca + ATPase activity was increased in exhausted muscle of trained rats (BONNER et al, 1976). Intense training did not affect skeletal muscle Ca 2+ ATPase concentration in human (MADSEN et al, 1994).…”

Section: Introductionmentioning

confidence: 73%

Effect of exercise on sarcoplasmic reticulum Ca<sup>2+</sup> transport in muscle of mouse lines long-term selected for different Performance traits

Küchenmeister¹,

Langhammer

Renne

et al. 2001

Arch. Anim. Breed.

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SummaryMouse lines used were long-term selected for high (DU-hTP) or low treadmill Performance (DU-ITP), or high locomotor open field activity (DU-hOF). The control line DU-Ks was maintained unselected. For each line 30 mice were used at 42 days of age. All lines were split into three groups: the first group was investigated without running exercise, the second had to run 500 m, and the third had to run until exhaustion on the treadmill. Compared to mice without running, in exhausted mice the maximal rate of SR Ca 2+ uptake of the m. rectus femoris decreases were only found in the mouse lines DU-ITP, and DU-hOF (28.5 %, and 36.3 %, respectively; p<0.05). The effect ofthe 500 m run was significant (p<0.05) in DU-hTP, but not in the other lines. An effect of exhausting exercise on Ca 2 * ATPase was detected only in DU-hOF with increased values after the exhaustive run. The hypothesis, that exercise induced alterations in Ca 2 * transport in muscle of mice selected for high activity (DU-hOF) or high running Performance (DU-hTP) are attenuated compared to mice which were not selected (DU-Ks) or selected for low Performance (DU-ITP), could not be verified.

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“…The mechanism involving the eects of training in protecting the mitochondria is not clear. It has been proposed that training reduces the accumulation of calcium in mitochondria (Bonner et al 1976) and increases the antioxidant capacity (Venditti and Di Meo 1997) of muscle ®bers.…”

Section: Discussionmentioning

confidence: 99%

Alterations in the expression and activity of creatine kinase-M and mitochondrial creatine kinase subunits in skeletal muscle following prolonged intense exercise in rats

2000

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Creatine kinase (CK) isoenzymes are important structural and energy metabolism components in skeletal muscle. In this study, CK isoenzyme alterations were examined in male rats, with an 8% body mass weight attached to their tail. The rats were either forced to swim for 5 h (5S, n = 51), or were pre-trained for 8 days and then forced to swim for 5 h (T5S, n = 48). Rats were sacrificed either immediately (0 h PS), 3 h (3 h PS), or 48 h post-swimming (48 h PS). Serum CK was increased significantly (P < 0.01) 6.2- and 2.0-fold at 0 h PS following the 5S and T5S protocols, respectively. However, training (T5S protocol) significantly (P < 0.01) decreased CK release. Soleus and white gastrocnemius (WG) CK activity was significantly decreased following the 5S protocol (P < 0.05), but not following the T5S protocol. The CK-M activity of the soleus muscle was significantly (P < 0.05) decreased at 0 h PS following both the 5S and T5S protocols, and returned to control values at 3 h PS. The CK-M activity of the WG was significantly (P < 0.05) decreased at 0 h PS following the 5S protocol. Sarcomeric mitochondrial CK (sCK-Mit) was decreased significantly (P < 0.01) at 0 h PS (20%), 3 h PS (14%), 24 h PS (22%), and 48 h PS (15%) following the 5S protocol. However, sCK-Mit was decreased significantly (P < 0.01) only at 0 h PS (7%) following the T5S. The results of this study demonstrate that prolonged intense exercise causes a loss of skeletal muscle CK-M and sCK-Mit activity and that training prior to the prolonged intense exercise attenuates the exercise-induced CK-M and sCK-Mit loss in both red and white skeletal muscles.

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Effects of Exercise Training and Exhaustion on 45ca2+ Uptake and Ca-Atpase Activity of Rat Skeletal Muscle Microsomal and Mitochondrial Fractions

Cited by 7 publications

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Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles

Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles

Effect of exercise on sarcoplasmic reticulum Ca<sup>2+</sup> transport in muscle of mouse lines long-term selected for different Performance traits

Alterations in the expression and activity of creatine kinase-M and mitochondrial creatine kinase subunits in skeletal muscle following prolonged intense exercise in rats

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Effects of Exercise Training and Exhaustion on 45ca2+ Uptake and Ca-Atpase Activity of Rat Skeletal Muscle Microsomal and Mitochondrial Fractions

Cited by 7 publications

References 0 publications

Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles

Effect of endurance training and acute exercise on sarcoplasmic reticulum function in rat fast- and slow-twitch skeletal muscles

Effect of exercise on sarcoplasmic reticulum Ca&lt;sup&gt;2+&lt;/sup&gt; transport in muscle of mouse lines long-term selected for different Performance traits

Alterations in the expression and activity of creatine kinase-M and mitochondrial creatine kinase subunits in skeletal muscle following prolonged intense exercise in rats

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Effect of exercise on sarcoplasmic reticulum Ca<sup>2+</sup> transport in muscle of mouse lines long-term selected for different Performance traits