Effects of Exhaustive Exercise and Aged Garlic Extract Supplementation on Weight, Adipose Tissue Mass, Lipid Profiles and Oxidative Stress in High Fat Diet Induced Obese Rats

Abstract

The purpose of this present study was to investigate the effects of long-term treadmill running and aged garlic extract supplementation on weight, adipose tissue mass, lipid profiles and oxidative stress after exhaustion in high fat diet-induced obese rats. Thirty male Sprague-Dawley rats (3 weeks old) were used as subjects. High fat diets were fed to all subjects for 6 weeks in order to induce obesity. The subjects were divided into five groups-N (normal diet group), HD (high fat diet group), E (exercise group), EA (exercise with AGE diet group) and A (AGE diet group). Aerobic exercise was performed through treadmill running and AGE at a dosage of 2.86 kg/g was administered to rats 30 min before every exercise for 4 weeks. After 4 weeks, all groups completed acute treadmill running (speed increasing gradually to 25 m/min, 15% uphill grade) until exhaustion. Immediately after exhaustive treadmill exercise, the weight, adipose tissue mass, lipid profiles and oxidative stress of the rats were assayed. At the end of 6 weeks of high fat diets, body weight and body weight gain were significantly higher in the high fat diet groups than in the normal diet group (p<0.001). At the end of treadmill exercise with AGE intake for 4 weeks, body weight gain, visceral and epididymal fat of the E and EA groups were significantly decreased compared to other groups (p<0.05). There were no significant differences in gastrocnemius and soleus. T-C, HDL-C, TG and LDL-C were not significant in any of the groups. TBARS was significantly lower in the A group than in the E group (p<0.05). These results indicated that body weight gain, visceral and epididymal fat decreased in the E and EA groups, and TBARS levels were lower in the A group than the E group. Regular aerobic exercise intervention with AGE supplementation may also modify the adipose weight and improve the oxidant stress in obese rats.

The Effects of Aerobic Exercise Training on Blood Lipid Profiles, Fibrinolytic Activities, and Nitric Oxide Levels in High-fat-diet induced Rats

“…Many studies suggested that moderate exercise intensity training reduces LDL-C level in the blood [19]. Interestingly, the present study identified that LDL-C was not significantly difference between EX and CON which can be supported by Lee et al [30] which suggested that the moderate intensity of aerobic exercise may positively affect LDL-C but it does not always reduced, and further suggested that the LDL-C level is the least sensitive markers of exercise induced improved lipid metabolism in rats.…”

Black Garlic and Its Bioactive Compounds on Human Health Diseases: A Review

“…Since a perfect cure or prevention of obesity still remains to be established and the majority of the anti-obesity drugs could have adverse effects, an exploration into the identification of new materials has shown a growing interest. BG extracts have been reported for their activity in reducing body weight, adipose tissue mass, serum triglyceride, total cholesterol, low-density lipoprotein, and plasma malondialdehyde in mice with high-fat-diet-induced obesity (Table 3) [25,29,101]. The fermentation of garlic by lactic acid bacteria ameliorated diet-induced obesity in db/db C57BL/6J mice by decreasing body weight (18%), lowered epididymal (36%), retroperitoneal (44%), and mesenteric adipose tissue mass (63%), respectively.…”