Effects of exogenous 24‐epibrassinolide to control grey mould and maintain postharvest quality of table grapes

Abstract

The potential of various concentrations of 24-epibrassinolide (EBR) treatments on the control of grey mould in artificial Botrytis cinerea inoculation test and on the maintenance of postharvest quality of table grapes without inoculation in cold storage were researched. In the cold storage, clusters of grapes with exogenous EBR application (EBR1, 0.1 mg L À1 EBR; EBR2, 0.4 mg L À1 EBR; EBR3, 0.8 mg L À1 EBR) were stored at 0 AE 1°C. Fruits' quality attributes including berry firmness, weight loss, berry drop, decay berry percentages, total sugar and titratable acidity were measured at 15, 30 and 60 days. The results showed that EBR treatments exhibited a higher berry firmness and a lower percentage of weight loss, berry drop and decay berry. In the artificial inoculation trial, single berries were immersed in EBR1, EBR2 and EBR3, then inoculated by spraying with a B. cinerea spore suspension and stored at 25 AE 2°C and 95% relative humidity. Disease severity (DS), lipid peroxidation and defence-related enzymes of these berries were analysed every 2 days. EBR-treated berries resulted in an increase in activities of defence-related enzymes including superoxide dismutase, peroxidase, catalase and phenylalanine ammonia-lyase compared with control berries inoculated by B. cinerea. Simultaneously, significant decreases in DS and production of superoxide anion radical and malondialdehyde were observed. These results suggested that exogenous application of EBR could play a protective role in controlling the grey mould of postharvest grapes and maintaining the postharvest quality during cold storage.EBR maintain the postharvest quality of grapes Q. Liu et al.

Effects of Ca(NO3)2 Stress on Mitochondria and Nitrogen Metabolism in Roots of Cucumber Seedlings

“…There were continuous increases in ROS in both of the cultivar's root mitochondria, but the salt-tolerant cucumber was significantly delayed in its increase of MDA. Similar results were also found in grape [29]. Excess MDA and ROS lead to the mitochondrial antioxidant system clearing ROS.…”

Postharvest treatments with γ‐aminobutyric acid, methyl jasmonate, or methyl salicylate enhance chilling tolerance of blood orange fruit at prolonged cold storage

“…The activity of PAL was evaluated by measuring the absorbance at 290 nm before and after incubation. One unit of PAL activity was described as the amount of enzyme that caused a 0.01 increase of absorbance at 290 nm per h. The specific PAL activity was expressed as U mg −1 protein …”

“…One unit of PAL activity was described as the amount of enzyme that caused a 0.01 increase of absorbance at 290 nm per h. The specific PAL activity was expressed as U mg −1 protein. 19 The extraction buffer for PPO activity contained 100 mM of potassium phosphate buffer (pH = 7.8) and 1% PVP. For enzyme extraction, 0.2 g of fruit peel was ground in with a mortar and pestle with 2 mL of extraction buffer and centrifuged at 13 000×g for 15 min at 4 ∘ C. The supernatant was used for measurement of PPO enzyme activity in a reaction mixture (3 mL), containing 100 μL of crude enzyme extract, 50 mM of potassium phosphate buffer (pH = 7) and 600 μL of 100 mM pyrocatechol solution which was maintained in a water bath at 25 ∘ C for 10 min.…”

Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes

“…It has been estimated that about 20%–25% of harvested fruits and vegetables decay from filamentous fungal infections during postharvest handling, even in developed countries; therefore, the control of such infections is critical (Calvo, Marco, Blanco, Oria, & Venturini, ). Gray mold, caused by Botrytis cinerea , is one of the most destructive diseases in grape production (Fu, Qu, Yang, & Zhang, ; Jaspers, Seyb, Trought, & Balasubramaniam, ; Liu et al, ). At present, the main prevention and control methods for grape gray mold include the use of chemical fungicides, such as cyprodinil (Latorre & Torres, ), pyrimethanil (Gil, Becker, & Viegas, ), fludioxonil (Zhang et al, ), and fenhexamid (Petit et al, ).…”