Effects of exogenous abscisic acid on the expression of citrus fruit ripening-related genes and fruit ripening

Wang, Xiaohua; Wu, Yin; Wu, Juxun; Chai, Lijun; Yi, Hualin

doi:10.1016/j.scienta.2015.12.024

Cited by 77 publications

(42 citation statements)

References 44 publications

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“…As a crucial hormone in the ripening process, ABA significantly affects carotenoid metabolism in tomato, mango, watermelon, and citrus fruits (Zhang et al ., , ; Zaharah et al ., ; Barickman et al ., ; Wang et al ., ,b). Several important genes involved in carotenoid metabolism, such as PSY3 from maize and DSM2 , a BCH gene from rice, were significantly induced by ABA treatments (Li et al ., ; Du et al ., ).…”

Section: Discussionmentioning

confidence: 99%

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An R2R3‐MYB transcription factor represses the transformation of α‐ and β‐branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

et al. 2017

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Although the functions of carotenogenic genes are well documented, little is known about the mechanisms that regulate their expression, especially those genes involved in α - and β-branch carotenoid metabolism. In this study, an R2R3-MYB transcriptional factor (CrMYB68) that directly regulates the transformation of α- and β-branch carotenoids was identified using Green Ougan (MT), a stay-green mutant of Citrus reticulata cv Suavissima. A comprehensive analysis of developing and harvested fruits indicated that reduced expression of β-carotene hydroxylases 2 (CrBCH2) and 9-cis-epoxycarotenoid dioxygenase 5 (CrNCED5) was responsible for the delay in the transformation of α- and β-carotene and the biosynthesis of ABA. Additionally, the expression of these genes was negatively correlated with the expression of CrMYB68 in MT. Further, electrophoretic mobility shift assays (EMSAs) and dual luciferase assays indicated that CrMYB68 can directly and negatively regulate CrBCH2 and CrNCED5. Moreover, transient overexpression experiments using leaves of Nicotiana benthamiana indicated that CrMYB68 can also negatively regulate NbBCH2 and NbNCED5. To overcome the difficulty of transgenic validation, we quantified the concentrations of carotenoids and ABA, and gene expression in a revertant of MT. The results of these experiments provide more evidence that CrMYB68 is an important regulator of carotenoid metabolism.

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Section: Discussionmentioning

confidence: 99%

“…Abscisic acit was extracted from the citrus flavedo from the fruits harvested in 2011 as recommended by Wang et al . (,b). Briefly, 0.2 g of ground sample was added to 750 μl of extraction buffer containing methanol : H 2 O : acetic acid (80 : 19 : 1, v/v/v) in a 2 ml tube.…”

Section: Methodsmentioning

confidence: 99%

An R2R3‐MYB transcription factor represses the transformation of α‐ and β‐branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

et al. 2017

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“…In grape berries, it was reported that pre-harvest exogenous MLT treatment significantly increased the polyphenolic content, antioxidant capacity, and related gene expressions, and improved the fruit maturity [12].Abscisic acid (ABA) is one of the crucial plant hormones, which play vital roles in fruit ripening and development. Studies reported that exogenous ABA promoted fruit coloration, including anthocyanin and flavanol accumulation during fruit ripening in apple [13], citrus [14], grape [15], litchi [16], strawberry [17], and tomato [18]. It was also reported that the transcriptional levels of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), chalcone synthase (CHS), chalcone isomerase (CHI), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) genes involved in polyphenolic biosynthesis were significantly increased by exogenous ABA treatment [16].…”

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confidence: 99%

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

et al. 2019

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Grape polyphenols contributing to more than half of the global polyphenol market were well studied; however, how melatonin (MLT), a potential plant hormone, and abscisic acid (ABA) affects polyphenols profile is still poorly understood. To explore whether these hormones are involved in polyphenolic biosynthesis, grape (Vitis vinifera cv. Kyoho) was exposed to MLT, ABA, and NDGA (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA. The present study provided new insights for improving flavonoids accumulation in agricultural production and its underlying mechanism.

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“…Peel degreening was shown to be accompanied by an increase in ABA content (Goldschmidt et al ., 1973), as well as in the expression of ABA biosynthetic and signalling elements (Rodrigo et al ., 2006; Kato et al ., 2006). In addition, exogenous ABA accelerated fruit ripening and enhanced fruit colour development (Wang et al ., 2016), while ABA-deficient citrus mutants showed a delay in the rate of peel degreening (Rodrigo et al ., 2003). In this study, ABA levels increased in ethylene-treated and low temperature-stored fruit (Fig.…”

Section: Discussionmentioning

confidence: 99%

Low temperature transcriptionally modulates natural peel degreening in lemon (Citrus limon L.) fruit independently of endogenous ethylene

Mitalo

Otsuki

Okada

et al. 2019

Preprint

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AbstractPeel degreening is an important aspect of fruit ripening in many citrus fruit, and earlier studies have shown that it can be advanced either by ethylene treatment or during low temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To understand how natural peel degreening is regulated in lemon (Citrus limon L.) fruit, flavedo transcriptome and physiochemical changes in response to either ethylene treatment or low temperature were studied. Ethylene treatment induced rapid peel degreening which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25°C, moderately low temperatures (5°C, 10°C, 15°C and 20°C) also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. On-tree peel degreening occurred along with environmental temperature drops, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.HighlightCitrus peel degreening is promoted by low temperature via modulation of multiple genes associated with chlorophyll degradation, carotenoid biosynthesis, photosystem disassembly, phytohormones and transcription factors without involving ethylene signalling.

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Effects of exogenous abscisic acid on the expression of citrus fruit ripening-related genes and fruit ripening

Cited by 77 publications

References 44 publications

An R2R3‐MYB transcription factor represses the transformation of α‐ and β‐branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

An R2R3‐MYB transcription factor represses the transformation of α‐ and β‐branch carotenoids by negatively regulating expression of CrBCH2 and CrNCED5 in flavedo of Citrus reticulate

Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

Low temperature transcriptionally modulates natural peel degreening in lemon (Citrus limon L.) fruit independently of endogenous ethylene

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