Effects of Exogenous Insoluble Glucan Primer on Insoluble Glucan Synthesis by Streptococcus mutans

Fukushima, Kazuo; Motoda, Ryuichi; Ikeda, Tadashi

doi:10.1177/00220345810600091201

Cited by 45 publications

(27 citation statements)

References 22 publications

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“…Hydroxyapatite Adsorption Assay S. mutans JC-2 (c) was grown for 20 h at 37 °C in the partially defined medium M4 [Fukushima et al, 1981]. The cells were col lected.…”

Section: Bacteriamentioning

confidence: 99%

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Anticaries Effects of Polyphenolic Compounds from Japanese Green Tea

Otake¹,

Makimura²,

Kuroki³

et al. 1991

Caries Res

236

148

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The dental caries inhibiting effect of the extract from Japanese green tea, one of the most popular drinks in Japan, was studied both in vitro and in vivo. The crude tea polyphenolic compounds (designated Sun-phenon®) from the leaf of Camellia sinensis were found to effectively inhibit the attachment of Streptococcus mutans strain JC-2 (serotype c) to saliva-coated hydroxyapatide discs. Sunphenon was also inhibitory to water-insoluble glucan formation from sucrose by crude glucosyltransferase of S. mutans JC-2 (c). Among the tea catechins tested, (––)-epigallocatechin gallate and (––)-epicatechin gallate showed the most potent inhibition of the glucosyltransferase activity. Finally, significantly lower caries scores were observed in specific pathogen free rats infected with S. mutans JC-2 (c) and fed a cariogenic diet and/or drinking water containing 0.05% Sunphenon as compared with control rats not receiving polyphenolic compounds.

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“…Hydroxyapatite Adsorption Assay S. mutans JC-2 (c) was grown for 20 h at 37 °C in the partially defined medium M4 [Fukushima et al, 1981]. The cells were col lected.…”

Section: Bacteriamentioning

confidence: 99%

“…The assay and preparation of crude GTase were based on the method of Fukushima et al [1981]. S. mutans JC-2 (c) was grown in M4 medium at 37 °C for 20 h, and after centrifugation, cold ab solute ethanol (-80°C) was added to the cell-free supernatant to give a final concentration of 40%.…”

Section: Gtuse Preparation and Assaymentioning

confidence: 99%

Anticaries Effects of Polyphenolic Compounds from Japanese Green Tea

Otake¹,

Makimura²,

Kuroki³

et al. 1991

Caries Res

236

148

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“…Colonies of the isolates grown on MS agar were similar in morphology to those of mutans-like streptococci. A water-insoluble material was produced following incubation with sucrose, and the culture supernatant was analysed by colorimetric assay (Van Handel, 1967;Fukushima et al, 1981). An adhesive, water-insoluble glucan was synthesized from sucrose by GTase, which was produced by strain NUM 1529 T .…”

mentioning

confidence: 99%

Streptococcus dentapri sp. nov., isolated from the wild boar oral cavity

Takada

Hayashi

Satō

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Four Gram-stain-positive, catalase-negative, coccoid-shaped isolates were obtained from the oral cavities of wild boars and characterized by phenotypic and phylogenetic studies. On the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed that the organisms are members of the genus Streptococcus, with Streptococcus equi subsp. equi ATCC 33398 T as their closest phylogenetic relative (94.7 % similarity). DNA-DNA hybridization analysis showed that the isolates displayed less than 10 % relatedness to Streptococcus equi subsp. equi DSM 20561 T . From the phylogenetic and phenotypic evidence, the four isolates represent a novel species of the genus Streptococcus, for which the name Streptococcus dentapri sp. nov. (type strain NUM 1529 T 5JCM 15752 T 5DSM 21999 T ) is proposed.Oral streptococci make up a proportion of the normal flora of the oral cavity in humans and animals. Among oral streptococci, the mutans group is one of the groups of micro-organisms responsible for dental caries (Hamada & Slade, 1980;Loesche, 1986). This group was proposed because its members share similar phenotypic characteristics. One of the causes of caries is an enzyme, glucosyltransferase (GTase), which is produced by this group of bacteria. GTase catalyses the formation of sticky water-insoluble glucans from sucrose. The glucans adhere oral bacteria to teeth and so lead to the formation of dental plaque. The bacteria continue to produce acid on the tooth surfaces and cause dental caries (Hamada & Slade, 1980;Loesche, 1986). Previously, we reported a novel mutans streptococci, Streptococcus orisuis, isolated from the oral cavity of a pig (Takada & Hirasawa, 2007). GTase gene (gtf) studies and rat caries experiments involving S. orisuis have been performed (ShinozakiKuwahara et al., 2008;Yamaguchi, 2008). Streptococcus dentirousetti , isolated from the bat oral cavity, showed a gtf gene sequence similar to that of S. orisuis (unpublished GenBank accession no. AB355819). The colonization of the oral cavity by mutans streptococci is assumed to result from sucrose intake. As wild boars are omnivorous animals, they will probably have the opportunity to ingest sucrose. We investigated the microflora of the oral cavities of wild boars, focusing on mutans streptococci.To investigate the oral microflora of wild boars, mitis salivarius agar (MS agar; Difco) and MS agar supplemented with 0.2 U bacitracin ml 21 (MSB agar) are widely used to isolate Streptococcus mutans as well as other oral streptococcal species. The four mutans streptococcus-like strains on MSB agar selected for this study were chosen randomly from 38 isolates of streptococci obtained from the oral cavities of eight generally healthy wild boars (three from Aomori and five from Tokushima prefectures in Japan). The strains were grown on brain heart infusion (BHI; Difco) agar supplemented with 5 % horse blood at 37 u C in an atmosphere of 95 % N 2 and 5 % CO 2 . Biochemical analysis was...

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“…S. mutans strains PS-14 (c), JC-2 (c), 10449 (c), B-13N (d), , , , KIR (g), and 6715 (g) were used in this study. An overnight culture, which was grown in brain heart infusion (BHI, Difco, Detroit, MI) broth anaerobically (95% N2, 5% C02), was transferred and grown aerobically in a partially defined medium, M4 (Fukushima et al, 1981a) for 20 hrs at 370C. M4 medium was found to minimize cell-bound GTase.…”

Section: Methodsmentioning

confidence: 99%

Effects of Certain Salts on Glucosyltransferase Synthesis by Streptococcus mutans Strain PS-14

Takada

Fukushima

1986

J Dent Res

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We investigated the effects of various salts on the synthesis of extracellular glucosyltransferase in a partially defined medium (M4) by Streptococcus mutans strain PS-14 and by certain other selected strains.The levels of glucosyltransferase produced by S. mutans strain PS-14 were found to increase to a maximum of seven-fold with increasing (NH4)2SO4 concentrations. However, at a 2% concentration of the salt, the level was stimulated 2.5-fold, and the growth was inhibited by 75%. A 1% concentration of MgSO4 or NaCl also caused an increase of glucosyltransferase of six-fold or 2.4-fold, respectively. However, for S. mutans strain B-13N, the enzyme production was not stimulated by (NH4)2S04, MgSO4, or NaCl. Moreover, the enzyme production by serotypes c, e, and f was found to be stimulated by 1% (NH4)2S04, whereas the enzyme production by serotypes d and g was not affected.J Dent Res 65(3):452-455, March, 1986 Introduction.

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Effects of Exogenous Insoluble Glucan Primer on Insoluble Glucan Synthesis by Streptococcus mutans

Cited by 45 publications

References 22 publications

Anticaries Effects of Polyphenolic Compounds from Japanese Green Tea

Anticaries Effects of Polyphenolic Compounds from Japanese Green Tea

Streptococcus dentapri sp. nov., isolated from the wild boar oral cavity

Effects of Certain Salts on Glucosyltransferase Synthesis by Streptococcus mutans Strain PS-14

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