Effects of exogenous <scp>GnRH</scp> administration on lambing performance of oestrus‐synchronized Kazak ewes during the breeding season

Zhang, Jing; Ma, Jun; Aibibaimu,; Wu, Xinglong; Yi-yong, Liu; Li, Xiangyun

doi:10.1111/rda.14306

Cited by 2 publications

(1 citation statement)

References 36 publications

(48 reference statements)

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“…This molecule can also be used to induce estrus synchronization ( 12 , 13 , 16 ). However, we previously found that using GnRH in sheep fixed-time artificial insemination and embryo transfer programs does not increase the embryo and embryo transfer programs decreased the embryo implantation rate ( 17 ). Unfortunately, the mechanism of its negative effect is still unclear.…”

Section: Introductionmentioning

confidence: 97%

GnRH-mediated suppression of S100A4 expression inhibits endometrial epithelial cell proliferation in sheep via GNAI2/MAPK signaling

Jiao,

Chu,

et al. 2024

Front. Vet. Sci.

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BackgroundGonadotrophin-releasing hormone (GnRH) administration significantly decreases the pregnancy rate of recipient ewes after embryo transfer, possibly because GnRH affects endometrial epithelial cell function. Therefore, this study investigated the effect of GnRH on endometrial epithelial cells.MethodsTranscriptome sequencing was used to determine the regulatory effect of GnRH on the ewe endometrium, and the S100A4 gene, which showed altered transcription, was screened as a candidate regulator of this effect. Endometrial epithelial cells were further isolated, the S100A4 protein was immunoprecipitated, and host proteins that interacted with S100A4 were identified by mass spectrometry. We further verified the effects of S100A4 and GNAI2 on the proliferation of endometrial epithelial cells via overexpression/knockdown experiments and subsequent CCK-8 and EdU assays. The effect of S100A4 deletion in endometrial cells on reproduction was verified in mice with S100A4 knockout.ResultsOur results showed that S100A4 gene transcription in endometrial cells was significantly inhibited after GnRH administration. GNAI2 was identified as a downstream interacting protein of S100A4, and S100A4 was confirmed to activate the MAPK signaling pathway to promote cell proliferation by targeting GNAI2.ConclusionGnRH can suppress the expression of S100A4 in the endometrium, consequently inhibiting the proliferation of endometrial cells through the S100A4/GNAI2/MAPK signaling pathway. These findings suggest a potential explanation for the limited efficacy of GnRH in promoting embryo implantation.

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Section: Introductionmentioning

confidence: 97%

GnRH-mediated suppression of S100A4 expression inhibits endometrial epithelial cell proliferation in sheep via GNAI2/MAPK signaling

Jiao,

Chu,

et al. 2024

Front. Vet. Sci.

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S100A4 targets PPP1CA/IL-17 to inhibit the senescence of sheep endometrial epithelial cells

Jiao,

Cui

et al. 2024

Front. Vet. Sci.

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BackgroundGonadotropin-releasing hormone (GnRH) is commonly used in animal reproduction and production, but it was previously reported that GnRH decreases the embryo implantation rate during artificial insemination or embryo transfer in sheep. In addition to the finding that GnRH can target S100A4 to inhibit endometrial epithelial cells proliferation, it was also found that endometrial cells were in poor condition and experienced cell death in S100A4 knockout mice, but the mechanism is unclear.MethodsThe protein PPP1CA, which interacts with S100A4, was detected by immunoprecipitation-mass spectrometry of overexpression and knockdown of S100A4 and PPP1CA. The effect of S100A4 and PPP1CA on cell senescence was detected by Galactosidase staining. To further reveal the mechanism effect of S100A4 and PPP1CA on cell senescence, transcriptome sequencing was conducted. Additionally, in vivo experiments were performed to assess PPP1CA protein expression in the endometrial tissue of S100A4 knockout mice.ResultsS100A4 inhibited cell senescence by activating PPP1CA, while PPP1CA overexpression suppressed the activation of the IL-17 signaling pathway. Inhibition of the IL-17 signaling pathway inhibited the senescence of endometrial cells.ConclusionS100A4 can target the PPP1CA/IL-17 signaling pathway and inhibit endometrial epithelial cell senescence.

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Effects of exogenous GnRH administration on lambing performance of oestrus‐synchronized Kazak ewes during the breeding season

Cited by 2 publications

References 36 publications

GnRH-mediated suppression of S100A4 expression inhibits endometrial epithelial cell proliferation in sheep via GNAI2/MAPK signaling

GnRH-mediated suppression of S100A4 expression inhibits endometrial epithelial cell proliferation in sheep via GNAI2/MAPK signaling

S100A4 targets PPP1CA/IL-17 to inhibit the senescence of sheep endometrial epithelial cells

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