Effects of Exogenously Applied Jasmonates on Growth and Intracellular pH in Maize Coleoptile Segments

Irving, Helen Ruth; Dyson, Gregory; McConchie, Robyn; Parish, Roger W.; Gehring, Chris

doi:10.1007/pl00007055

Cited by 17 publications

(12 citation statements)

References 32 publications

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“…Although ethylene biosynthetic genes were unaffected by GM, GM upregulated several negative regulators of ethylene-responsive genes (EBF1, ERF3, and ERF4; Fujimoto et al 2000;Potuschak et al 2003). The affect of JA trt on auxin metabolism and the regulation of meristem organization categories are consistent with JA's regulatory role in ordinary growth and development (Irving et al 1999;Ulloa et al 2002).…”

Section: Resultsmentioning

confidence: 61%

Local and systemic transcriptome responses to herbivory and jasmonic acid in Populus

Babst

Sjödin

Jansson

et al. 2009

Tree Genetics & Genomes

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We used DNA microarrays to examine local and systemic transcriptional responses to herbivory by gypsy moth larvae (GM) and exogenous jasmonic acid (JA trt ) in leaves of Populus nigra L. to identify candidate signaling and defense genes and also to examine primary metabolism, as might relate to tolerance of damage. GM and JA trt altered expression of over 800 genes, most of which have putative roles in defense signaling, secondary metabolism, and primary metabolism. Additionally, numerous uncharacterized genes responded to herbivory, providing a rich resource for future studies. There was limited overlap (14%) between the responses to GM and JA trt . GM did, however, result in strong upregulation of genes involved not only in JA biosynthesis but also abscisic acid biosynthesis and other signaling pathways. GM induced known resistance transcripts, including polyphenolic biosynthetic genes, proteinase inhibitors, and amino acid deaminases. According to GOStats pathway level analysis, GM altered primary metabolism, including aromatic amino acid biosynthesis, fatty acid β-oxidation, and carbohydrate and organic acid metabolism. These alterations may be related to increased demands for substrate for secondary metabolites or may serve a tolerance-related role. Responses were more intense locally in treated leaves than in untreated (systemic) leaves and systemic responses were mostly a subset of the genes induced locally. A stronger local response might be needed to cope with localized stresses and wound healing. Since Populus in general and this clone in particular are known for their systemic induced resistance, genes induced both locally and systemically may be the highest quality candidates for resistance.

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Section: Resultsmentioning

confidence: 61%

Local and systemic transcriptome responses to herbivory and jasmonic acid in Populus

Babst

Sjödin

Jansson

et al. 2009

Tree Genetics & Genomes

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“…IAA induced growth is inhibited by JA in monocotyledons (Miyamoto et al 1997). This is attributed to inhibition of cell wall polysaccharide synthesis (Ueda et al 1994(Ueda et al , 1995 and pH increase (Irving et al 1999). For both processes IAA has been shown to act antagonistically (Kutschera and Briggs 1987;Brummer et al 1985).…”

Section: Discussionmentioning

confidence: 97%

Cholodny–Went revisited: a role for jasmonate in gravitropism of rice coleoptiles

Gutjahr

Riemann

Müller

et al. 2005

Planta

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Gravitropism is explained by the Cholodny-Went hypothesis: the basipetal flow of auxin is diverted laterally. The resulting lateral auxin gradient triggers asymmetric growth. However, the Cholodny-Went hypothesis has been questioned repeatedly because the internal auxin gradient is too small to account for the observed growth asymmetry. Therefore, an additional gradient in indolyl-3-acetic acid (IAA) sensitivity has been suggested (Brauner and Hager in Planta 51:115-147, 1958). We challenged the Cholodny-Went hypothesis for gravitropism of rice coleoptiles (Oryza sativa L.) and found it to be essentially true. However, we observed, additionally, that the two halves of gravitropically stimulated coleoptiles responded differentially to the same amount of exogenous auxin: the auxin response is reduced in the upper flank but normal in the lower flank. This indicates that the auxin-gradient is amplified by a gradient of auxin responsiveness. Hormone contents were measured across the coleoptile by a GC-MS/MS technique and a gradient of jasmonate was detected opposing the auxin gradient. Furthermore, the total content of jasmonate increased during the gravitropic response. Jasmonate gradient and increase persist even when the lateral IAA gradient is inhibited by 1-N-naphtylphtalamic acid. Flooding with jasmonate delays the onset of gravitropic bending. Moreover, a jasmonate-deficient rice mutant bends more slowly and later than the wild type. We discuss a role of jasmonate as modulator of auxin responsiveness in gravitropism.

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“…Intriguingly, this inhibition of auxin-induced growth seems to be specific for monocotyledonous plants (for review, see Miyamoto et al, 1997). In studies performed with Graminean coleoptiles, the effect of jasmonate was attributed to inhibited synthesis of cell wall polysaccharides (Ueda et al, 1994(Ueda et al, , 1995 and to a shift in the intracellular pH (Irving et al, 1999). Both processes are well known to be promoted by auxin (Brummer et al, 1985;Felle et al, 1986;Kutschera and Schopfer, 1986;Kutschera and Briggs, 1987;Gehring et al, 1990;Frohnmeyer et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Impaired Induction of the Jasmonate Pathway in the Rice Mutant hebiba

et al. 2003

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The elongation of rice (Oryza sativa) coleoptiles is inhibited by light, and this photoinhibition was used to screen for mutants with impaired light response. In one of the isolated mutants, hebiba, coleoptile elongation was stimulated in the presence of red light, but inhibited in the dark. Light responses of endogenous indolyl-3-acetic acid and abscisic acid were identical between the wild type and the mutant. In contrast, the wild type showed a dramatic increase of jasmonate heralded by corresponding increases in the content of its precursor o-phytodienoic acid, whereas both compounds were not detectable in the mutant. The jasmonate response to wounding was also blocked in the mutant. The mutant phenotype was rescued by addition of exogenous methyl jasmonate and o-phytodienoic acid. Moreover, the expression of O. sativa 12-oxophytodienoic acid reductase, an early gene of jasmonic acid-synthesis, is induced by red light in the wild type, but not in the mutant. This evidence suggests a novel role for jasmonates in the light response of growth, and we discuss a cross-talk between jasmonate and auxin signaling. In addition, hebiba represents the first rice mutant in which the induction of the jasmonate pathway is impaired providing a valuable tool to study the role of jasmonates in Graminean development.

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Effects of Exogenously Applied Jasmonates on Growth and Intracellular pH in Maize Coleoptile Segments

Cited by 17 publications

References 32 publications

Local and systemic transcriptome responses to herbivory and jasmonic acid in Populus

Local and systemic transcriptome responses to herbivory and jasmonic acid in Populus

Cholodny–Went revisited: a role for jasmonate in gravitropism of rice coleoptiles

Impaired Induction of the Jasmonate Pathway in the Rice Mutant hebiba

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