Effects of exposure to 1.8 GHz radiofrequency field on the expression of Hsps and phosphorylation of MAPKs in human lens epithelial cells

Yu, Yibo; Yao, Ke; Wu, Wei; Wang, Kaijun; Chen, Guangdi; Lu, Di

doi:10.1038/cr.2008.306

Cited by 14 publications

(11 citation statements)

References 13 publications

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“…Following this paradigm, we present a novel methodology for indirectly investigating possible quantum effects in biological systems by applied static and alternating magnetic fields that induce changes in magnetically sensitive free radical pairs in biochemical reactions. Some evidence shows the effects of such exposures on cellular morphology, growth curves, and protein expression, implying an underlying metabolic influence [4]–[7]. The effect of weak magnetic fields on cellular metabolic processes is not well understood and little is known about how magnetic fields influence reaction rates in oxidative metabolism [8]–[10].…”

Section: Introductionmentioning

confidence: 99%

Spin Biochemistry Modulates Reactive Oxygen Species (ROS) Production by Radio Frequency Magnetic Fields

et al. 2014

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The effects of weak magnetic fields on the biological production of reactive oxygen species (ROS) from intracellular superoxide (O2 •−) and extracellular hydrogen peroxide (H2O2) were investigated in vitro with rat pulmonary arterial smooth muscle cells (rPASMC). A decrease in O2 •− and an increase in H2O2 concentrations were observed in the presence of a 7 MHz radio frequency (RF) at 10 μTRMS and static 45 μT magnetic fields. We propose that O2 •− and H2O2 production in some metabolic processes occur through singlet-triplet modulation of semiquinone flavin (FADH•) enzymes and O2 •− spin-correlated radical pairs. Spin-radical pair products are modulated by the 7 MHz RF magnetic fields that presumably decouple flavin hyperfine interactions during spin coherence. RF flavin hyperfine decoupling results in an increase of H2O2 singlet state products, which creates cellular oxidative stress and acts as a secondary messenger that affects cellular proliferation. This study demonstrates the interplay between O2 •− and H2O2 production when influenced by RF magnetic fields and underscores the subtle effects of low-frequency magnetic fields on oxidative metabolism, ROS signaling, and cellular growth.

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Section: Introductionmentioning

confidence: 99%

Spin Biochemistry Modulates Reactive Oxygen Species (ROS) Production by Radio Frequency Magnetic Fields

et al. 2014

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“…These three pathways are all linked to cell cycle control, suggesting that RF EMF can interfere with the routine cellular functions associated with cell cycle control; cytoskeleton and extracellular matrix management, apoptosis, and cellular replication. There is evidence from single endpoint studies reporting changes in gene and/or protein expression after RF EMF exposure related to apoptosis and/or cell cycle control (10, 15, 16, 19, 20, 23, 24, 57, 58). …”

Section: Discussionmentioning

confidence: 99%

“…The ERK 1 and 2 are MAPKs that function in cellular proliferation, differentiation, and survival, and their inappropriate activation is a common occurrence in human cancers. Several authors (15, 16, 19, 24, 25, 46, 59) have shown changes in ERK (either transcription or activity), following exposure to RF EMF fields. The c-Jun N-terminal kinases (JNKs) are also MAPKs regulated by specific MAPK kinases (MKKs) and MKK kinases (MKKKs) that phosphorylate and regulate the activity of transcription factors and regulatory proteins in the cell.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, the p38 MAPKs are involved in cellular replication and differentiation and, along with the JNKs, are responsive to stress stimuli, such as mitogens, ultraviolet radiation, and heat shock. These have been extensively studied in the RF and ELF EMF literature with mixed results where some researchers have seen changes in p38 MAPKs following RF EMF exposure (19), while others have not (5, 15, 16, 24, 25), even though most of the studies demonstrated changes in heat-shock proteins.…”

Section: Discussionmentioning

confidence: 99%

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The Use of Signal-Transduction and Metabolic Pathways to Predict Human Disease Targets from Electric and Magnetic Fields Using in vitro Data in Human Cell Lines

Parham

Portier²,

Chang

et al. 2016

Front. Public Health

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Using in vitro data in human cell lines, several research groups have investigated changes in gene expression in cellular systems following exposure to extremely low frequency (ELF) and radiofrequency (RF) electromagnetic fields (EMF). For ELF EMF, we obtained five studies with complete microarray data and three studies with only lists of significantly altered genes. Likewise, for RF EMF, we obtained 13 complete microarray datasets and 5 limited datasets. Plausible linkages between exposure to ELF and RF EMF and human diseases were identified using a three-step process: (a) linking genes associated with classes of human diseases to molecular pathways, (b) linking pathways to ELF and RF EMF microarray data, and (c) identifying associations between human disease and EMF exposures where the pathways are significantly similar. A total of 60 pathways were associated with human diseases, mostly focused on basic cellular functions like JAK–STAT signaling or metabolic functions like xenobiotic metabolism by cytochrome P450 enzymes. ELF EMF datasets were sporadically linked to human diseases, but no clear pattern emerged. Individual datasets showed some linkage to cancer, chemical dependency, metabolic disorders, and neurological disorders. RF EMF datasets were not strongly linked to any disorders but strongly linked to changes in several pathways. Based on these analyses, the most promising area for further research would be to focus on EMF and neurological function and disorders.

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“…RF radiation on rabbit lens showed various biochemical changes in the form of altered protein expression on cell membranes of lens epithelial cells, which declined cell proliferation, and lens epithelial cells damage and opacity [6][7][8]. The heat shock proteins, HSP-70 and HSP-27, in lens epithelial cells [9,10] are reported to increase due to radiation exposure. Oxidative stress is a leading cause for cataractogenesis.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ultrahigh Frequency Radiation Emitted from 2G Cell Phone on Developing Lens of Chick Embryo: A Histological Study

D'Silva

Swer

Anbalagan

et al. 2014

Advances in Anatomy

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A Mobile phone in operation emits a pulsed radiofrequency electromagnetic field which is absorbed into the user's body particularly the head region. Contradictory scientific reports on the health effect of nonionizing radiations on biological tissues have prompted to undertake the present study to evaluate the damage in the developing lens of a chick embryo following exposure to radiation emitted from a 2G cell phone. Fertilized chick embryos were incubated in two groups in a standard egg incubator. The experiment group was exposed to radiation emitted from a 2G cell phone. On completion of scheduled duration, the embryos were collected and processed for routine histological studies. The 9th to 12th day chick embryo eyes were processed for assessment of DNA damage using the alkaline comet assay technique. The lens thickness and the equatorial diameter were measured using oculometer and statistically compared for both groups. In the present study, the exposure of chick embryos to a 2G cell phone caused structural changes in lens epithelial cells, formation of cystic cells and spaces, distortion of lens fibers, and formation of posterior aberrant nuclear layer. The DNA damage in the developing eyes of the experiment group assessed by comet assay was highly significant.

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Effects of exposure to 1.8 GHz radiofrequency field on the expression of Hsps and phosphorylation of MAPKs in human lens epithelial cells

Cited by 14 publications

References 13 publications

Spin Biochemistry Modulates Reactive Oxygen Species (ROS) Production by Radio Frequency Magnetic Fields

Spin Biochemistry Modulates Reactive Oxygen Species (ROS) Production by Radio Frequency Magnetic Fields

The Use of Signal-Transduction and Metabolic Pathways to Predict Human Disease Targets from Electric and Magnetic Fields Using in vitro Data in Human Cell Lines

Effect of Ultrahigh Frequency Radiation Emitted from 2G Cell Phone on Developing Lens of Chick Embryo: A Histological Study

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