Effects of Exposure to a 50Hz Electric Field on Plasma Levels of Lactate, Glucose, Free Fatty Acids, Triglycerides and Creatine Phosphokinase Activity in Hind-Limb Ischemic Rats

Abstract

ABSTRACT. We previously reported that extremely low frequency electric fields (ELF-EFs) affect energy metabolism in stressed conditions. To further confirm this, the effect of exposure to ELF-EFs on the experimental ischemic rat was examined. The test was based on a comparison of rats treated with EF alone, ischemic surgery alone, the combination of EF with ischemic surgery, or no treatment (double sham). The EF condition used in this study was an alternating current of 50 Hz EF at 17 500 V/m intensity for 15 min per day. The exposure to EF in ischemic rats significantly decreased plasma levels of free fatty acids and triglycerides, compared to those of the no treatment or EF alone group. The plasma lactate levels of two ischemic groups peaked on experimental day-4 and gradually decreased until the end of the study. The changes in the lactate levels induced by ischemia did not show any difference between rats treated with ischemia alone or a combination of ischemia with an EF. Any changes in plasma levels of glucose and creatine phosphokinase activity were not influenced by EF treatment. These results indicate that the EF effect on glycolysis parameters, plasma lactate or glucose levels, does not appear in a highly stressed condition and that EF effects varied dependent on the condition of organism but ELF-EF used in this study have impact on lipid metabolism parameter in a hind-limb ischemic rat. However, further studies are needed to elucidate the association of ELF-EF with the lipid metabolism system. KEY WORDS: bioelectromagnetism, electric field, 50 Hz, ischemia, lipid metabolism.J. Vet. Med. Sci. 67(10): 969-974, 2005 The biological effects of an extremely low frequency electric field (ELF-EF) have become a matter of interest and debate as worldwide distribution of electricity, which markedly increased during the last half century, resulted in increased human exposure to electromagnetic fields (EMFs) [36]. Clinical techniques using electric fields (EFs) have been developed in disciplines such as complementary and integrative medical therapies [13][14][15][16]27]. In Japan, EF therapy has been utilized since 1972, when Hara et al. developed EF exposure equipment (Healthtron TM , Hakuju Institute for Health Science, Tokyo, Japan) [15], which was approved by the Ministry of Health, Labour and Welfare (Approval number 14700BZZ00904). Recently, the palliative effects of EF therapy on several types of pain (headache, stiff shoulders and stomachache) in over a thousand cases have been reported [16]. Publications explaining the mechanism(s) of the response of an exposure to an EF have suggested that either the EF-induced electric current or the perception of the EF through the skin surface acts as a trigger on cellular, humoral or behavioral responses [17, 18, 22-24, 32, 40, 44, 45].In a recent study, we reported evidence that an EF participates in the response to stress and also affects energy metabolism by suppressing the anabolism of lactate, not the acceleration of the catabolism of lactate [17]. However, ...

Effects of Acute and Chronic Low Frequency Electromagnetic Field Exposure on PC12 Cells during Neuronal Differentiation

“…Also, under our experimental conditions, it was possible to note a significant modification in ROS production in undifferentiated PC12 cells while the differentiated phenotype seemed not to be affected. Moreover, in vivo experiments also appear to confirm that chronic exposure to ELF-EMFs can generate an oxidative stress status [31,[52][53][54][55][56][57][58][59][60][61]. In addition, the presence of a "stress" status is also demonstrated by the fact that the enzymatic activity of catalase, involved in the catalytic removal of ROS accumulated as a possible effect of ELF-EMF exposure, was decreased during chronic exposure.…”

Time-dependent effects of exposure to static magnetic field on glucose and lipid metabolism in rat

“…Pierre et al (2008) and Tsuji et al (1996). Other findings showed that blood glucose level was not affected (Sutter et al 1987;Harakawa et al 2005;Mehrshad et al 2007), or decreased (Gorczynska et al 1989;Laitl-Kobierska et al 2002;Sakurai et al 2005) after SMF exposition. We can hypothesize this difference in results by the different strength and/or the duration of exposition to the magnetic field.…”

Effect of Magnetic Water on the Performance of Lactating Goats

“…Increasing both the electric conductivity and the dielectric constant of water was documented [57]. Some researchers reported that magnetic treatment affect water properties such as light absorbance, pH, surface tension [58] and amount of oxygen dissolved in water [59]. Normal water has a pH level of about 7, whereas magnetic water can reach pH to 9.2 following the exposure to 7000 gauss strength magnet for a long period of time [8].…”