Effects of exposure to hyperbaric oxygen on oxidative stress in rats with type II collagen-induced arthritis

Abstract

Arthritis was induced in 9-week-old female Dark Agouti rats by injecting type II collagen. Serum levels of the derivatives of reactive oxygen metabolites (dROMs), which are oxidative stress markers, and C-reactive protein (CRP) in arthritic rats that were exposed to a pressure of 1.25 atmospheres absolute and an oxygen concentration of 36% for 3 weeks (arthritis + HBO group) were compared to those of control rats (control group) and arthritic rats that were not exposed to hyperbaric oxygen (arthritis group). The body weights of the arthritis and arthritis + HBO groups were lower than that of the control group, whereas no difference in the body weight was observed between the arthritis and arthritis + HBO groups. The serum levels of dROMs and CRP in the arthritis group were higher than those in the control and arthritis + HBO groups. No difference in the serum level of CRP was observed between the control and arthritis + HBO groups. These results indicate that the conditions of hyperbaric oxygen exposure used in this study are effective for reducing the levels of reactive oxygen species, which are overproduced during arthritis.

Modulation of C-reactive protein and tumour necrosis factor-alpha in collagen-induced arthritis in Dark Agouti rats: impact of collagen concentration on severity of arthritis

“…C-reactive protein has been used as one of the best laboratory measures of inflammation in a variety of clinical settings like myocardial infarction and rheumatoid arthritis [12,14,15]. The results of the present research confirm the earlier research that CRP increases in arthritis.…”

“…Previous studies have reported that the body weights of collagen-induced arthritis gradually decrease following immunization. Body weights will be lowest after 3 weeks of immunization and subsequently recover to control levels [12,13]. However, our study did not show a gradual decrease in body weight after 2 or 3 weeks of immunization except for 3 mg/kg body weight, where a significant decrease in body weight was seen around 30 days.…”

“…Higher concentration of CRP will indicate increased joint changes in arthritis. The most pertinent point of the present research is the higher level of CRP with increasing concentration of collagen in suspension, which confirms that CRP will be an ideal marker for monitoring severity of inflammation in collagen-induced arthritis in Dark Agouti rats [12,17]. In the present study, proinflammatory cytokine, tumour necrosis factor-a (TNF-a) concentration in plasma was measured by enzyme linked assay.…”

Lifestyle-related disease and skeletal muscle: A review

“…Overproduction of ROS in SHR rats is inhibited by hyperbaric oxygen 66) . Increased oxidative stress markers and C-reactive protein (CRP) are observed in female Dark Agouti rats with arthritis, which is induced by injecting type II collagen 70) . In contrast, increased oxidative stress markers and CRP of rats with arthritis are inhibited by hyperbaric oxygen.…”

“…In contrast, increased oxidative stress markers and CRP of rats with arthritis are inhibited by hyperbaric oxygen. Hyperbaric oxygen is effective for reducing the overproduction of ROS in rats with type II collagen-induced arthritis 70) . Fig.…”