Effects of exposure to methylglyoxal on sperm motility and embryonic development after fertilization in mice

Nakano, Tatsuya; Kono, Mizuki; Segawa, Kazuki; Kurosaka, Satoshi; Nakaoka, Yoshiharu; Morimoto, Yoshiharu; Mitani, Tomohiko

doi:10.1262/jrd.2020-150

Cited by 6 publications

(4 citation statements)

References 50 publications

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“…In the lower part of the suspension, a significant decrease was noted when using MG at a concentration of 1mM and 5mM (a decrease from 37.2% to 19.8% and from 37.2% to 1.0%). In addition, it was found that the developmental capacity of oocytes fertilized with sperm treated with 500μM and 1 mM MG to the blastocyst stage significantly decreased in a concentration-dependent manner (51.4% and 35.0%, respectively), which confirms the effect of MG on embryonic development after fertilization [19].…”

Section: Resultssupporting

confidence: 52%

Diet and its association with reduced semen quality

Ochyra

Łopuszyńska

Pawlicki

et al. 2022

J Educ Health Sport

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Introduction: Infertility is defined as the inability to conceive a child for at least 12 months of regular unprotected intercourse and may affect up to 15% of couples of reproductive age. Reduced semen quality may be caused by trauma, inflammation, or an inappropriate lifestyle. Research suggests that diet may be a factor in influencing sperm parameters.Material and methods: The literature of the PubMed scientific base was searched in 2015-2022 using the following keywords: semen, diet, infertility.Results: Analyzes of eating patterns showed that eating a diet high in red meat, high-fat dairy, sweet energy drinks, and snacks could negatively affect semen, and a pattern based on the consumption of fish, chicken, vegetables, fruits, legumes, and whole grains was associated with with an increased concentration of sperm. A high-fat diet (HFD) leads to obesity, endocrine disorders, dysbiosis of the intestinal microflora, and may be a factor that activates autophagy, which results in reduced sperm quality. A ketogenic diet can positively affect the semen of obese, infertile men. Studies analyzing the Mediterranean diet suggest that higher compliance rates are associated with a higher percentage of mobility as well as a reduced risk of low sperm concentration. The way you prepare your meals can also play a role as glycation end products are produced when grilling, baking and frying, which can damage sperm DNA.Conclusions: Diet may be an important factor influencing the quality of sperm in men, however, there is a need for further extensive, multi-center studies that will systematize this knowledge, which will enable the creation of specific nutritional recommendations for this group of people.

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Section: Resultssupporting

confidence: 52%

Diet and its association with reduced semen quality

Ochyra

Łopuszyńska

Pawlicki

et al. 2022

J Educ Health Sport

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“…Similarly, mitigation of hyperglycaemia also results in reduced mitogen‐activated protein kinase activity, 122,123 a pathway identified to be activated by RAGE in somatic cells 124 . In vitro, incubation of spermatozoa with AGE‐forming molecules in both non‐diabetic rodents and humans is associated with reduced sperm motility, mitochondrial activity and fertilisation rates and increased sperm DNA damage 125,126 …”

Section: Why There May Be Descrepancies As To the Effect Of Obesity O...mentioning

confidence: 99%

“…non-diabetic rodents and humans is associated with reduced sperm motility, mitochondrial activity and fertilisation rates and increased sperm DNA damage. 125,126 Although type II diabetes is clearly associated with increased sperm DNA damage, there is a significant knowledge gap as to the effect of insulin-resistant obesity.…”

Section: Insulin Resistancementioning

confidence: 99%

Sperm DNA damage: The possible link between obesity and male infertility, an update of the current literature

et al. 2023

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Obesity prevalence worldwide is increasing significantly. Whilst maternal obesity has clear detrimental impacts on fertility, pregnancy and foetal outcomes, more recently there has been an increasing focus on the role of paternal obesity in human fertility. Recent meta‐analyses have indicated that obesity in men negatively affects basic sperm parameters such as sperm count, concentration and motility, increases the incidence of infertility and reduces the chances of conception. Sperm DNA damage, typically characterised by DNA strand breaks and oxidation of DNA nucleotides, is a specialised marker of sperm quality that has been independently associated with recurrent miscarriage, reduced assisted reproduction success and increased mutational loads in subsequent offspring. Whilst, there are still conflicting data in humans as to the association of obesity in men with sperm DNA damage, evidence from rodent models is clear, indicating that male obesity increases sperm DNA damage. Human data are often conflicting because of the large heterogeneity amongst studies, the use of body mass index as the indicator of obesity and the methods used for detection of sperm DNA damage. Furthermore, comorbidities of obesity (i.e., heat stress, adipokines, insulin resistance, changes in lipids, hypogonadism and obstructive sleep apnoea) are also independently associated with increased sperm DNA damage that is not always modified in men with obesity, and as such may provide a causative link to the discrepancies amongst human studies. In this review, we provide an update on the literature regarding the associations between obesity in men and fertility, basic sperm parameters and sperm DNA damage. We further discuss potential reasons for the discrepancies in the literature and outline possible direct and indirect mechanisms of increased sperm DNA damage resulting from obesity. Finally, we summarise intergenerational obesity through the paternal linage and how sperm DNA damage may contribute to the transmission.

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“…(3,4) Complications include diabetes, (5)(6)(7) neurodegenerative diseases (Parkinson's disease and Alzheimer's disease), (8) and male infertility. (9) The most common method of detecting carbonyl stress is the detection of methylglyoxal, which has the highest carbonyl stress. (10) High-performance liquid chromatography (HPLC) has been used to detect methylglyoxal.…”

Section: Introductionmentioning

confidence: 99%

Development of a Simple Fluorescence Sensing System for Carbonyl Stress in Urine

Huang¹,

Sun²,

Lee³

et al. 2021

Sensors and Materials

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In this study, we developed a simple fluorescence sensing system to rapidly measure the total carbonyl stress in urine because carbonyls may form advanced glycation end products, leading to diseases. The traditional method for detecting carbonyl stress has been high-performance liquid chromatography (HPLC), which detects glyoxal, methylglyoxal, and 3-deoxyglucosone contents. The excitation and emission wavelengths used in the sensing system to monitor aldehyde content in urine were 340 and 500 nm, respectively. Rat urine samples were derivatized with 5,6-diamino-2,4-hydroxypyrimidine sulfate dihydrate. A 20 µL sample was added to a carrier composed of polydimethylsiloxane for testing, and only 1 s was required for detection. According to the fluorescence intensity results, the calibration curve for the total content of all aldehydes in the urine had a good degree of linearity with the fluorescence intensity (R 2 = 0.9879). In addition, the urine of diabetic and healthy rats was tested, and the correlation coefficient between the total carbonyl content detected by the simple fluorescence system and HPLC reached 0.8132. Collectively, our findings demonstrated that our system can rapidly measure total carbonyl stress in urine with a small sample volume.

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Effects of exposure to methylglyoxal on sperm motility and embryonic development after fertilization in mice

Cited by 6 publications

References 50 publications

Diet and its association with reduced semen quality

Diet and its association with reduced semen quality

Sperm DNA damage: The possible link between obesity and male infertility, an update of the current literature

Development of a Simple Fluorescence Sensing System for Carbonyl Stress in Urine

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