Effects of exposure to noise on ion movement in guinea pig cochlea

Konishi, T.; Salt, Alec N.; Hamrick, Philip E.

doi:10.1016/0378-5955(79)90004-2

Cited by 64 publications

(29 citation statements)

References 22 publications

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“…The hypothesis has been supported by numerous experiments (Konishi et al 1979;Salt and Konishi 1979;Syka et al 1981). A decreased rate of K + secretion by the strial marginal cells would be expected to cause a reduction in the positive component of the EP (Wangemann 2002).…”

Section: Discussionmentioning

confidence: 86%

Age-Related Changes in Cochlear Endolymphatic Potassium and Potential in CD-1 and CBA/CaJ Mice

Marcus

2003

JARO - Journal of the Association for Research in Otolaryngolog

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The CD-1 mouse strain is known to have early onset of hearing loss that is progressive with aging. We sought to determine whether a disturbance of K + homeostasis and pathological changes in the cochlear lateral wall were involved in the age-related hearing loss (AHL) of CD-1 as compared to the CBA/CaJ strain which has minimal AHL. In the present study, the endocochlear potential (EP) and endolymphatic K + concentration ([K + ] e ) were measured in both strains of mice with double-barrel microelectrodes at ''young'' (1-2 mo) and ''old'' (5-9 mo) ages. CBA/ CaJ mice displayed no changes with aging in EP and [K + ] e of the basal turn. In the apical turn, there was a small positive shift of the EP (10 mV) with aging under both normoxic and acute anoxic conditions ()EP), without any change of [K + ] e . Further, there were no obvious pathological changes in the lateral wall of CBA/CaJ mice. By contrast, old CD-1 mice displayed a significantly reduced [K + ] e by 30% in both basal and apical turns with no significant changes in normoxic EP. The )EP in the apical turn was significantly reduced in magnitude by 6 mV. A severe loss of cells with aging was observed in the region of type IV fibrocytes of the apical and basal turns and of type II fibrocytes in the basal turn. A complete degeneration of organ of Corti was also observed at the basal turn of old CD-1 mice, as well as a basalward decline of spiral ganglion neuron density. The pathological changes in spiral ligament of CD-1 mice were similar to those of an inbred mouse strain C57BL/6J that expresses an AHL gene (ahl) and might be a primary etiology of AHL of CD-1 mice. These findings have ramifications for our understanding of AHL and for interpretation of genetic mutations in a CD-1 background.

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Section: Discussionmentioning

confidence: 86%

Age-Related Changes in Cochlear Endolymphatic Potassium and Potential in CD-1 and CBA/CaJ Mice

Marcus

2003

JARO - Journal of the Association for Research in Otolaryngolog

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“…This is not surprising, since noise imposes a number of other types of injury that mostly affect the organ of Corti (e.g., Nordmann et al 2000;Wang et al 2002). Overt breaches in the organ of Corti, which might directly link organ of Corti injury and EP reduction (Salt and Konishi 1979;Hirose and Liberman 2003), appear infrequent in BALBs under the exposure conditions used here (Ohlemiller et al 2011). Although strain variation in the effects of noise on thresholds at mid-and high-frequencies likely reflect multiple susceptibility factors, more straightforward effects of an acute EP change might be observed at lower frequencies.…”

Section: Strain Distribution Of Acute Cap Thresholds and Eps In Noisementioning

confidence: 97%

“…However, relatively few have included EP measurement (e.g., Vassout 1984;Ikeda et al 1988;Ahmad et al 2003), and parametric studies have been performed only in guinea pigs (Konishi et al 1979;Salt and Konishi 1979;Ide and Morimitsu 1990;Wang et al 1992) and mice (Wang et al 2002;Hirose and Liberman 2003). In guinea pigs, the EP may temporarily rise or fall after noise, depending apparently on noise parameters.…”

Section: Introductionmentioning

confidence: 99%

QTL Mapping of Endocochlear Potential Differences between C57BL/6J and BALB/cJ mice

Ohlemiller

Kiener

Gagnon

2016

JARO

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We reported earlier that the endocochlear potential (EP) differs between C57BL/6J (B6) and BALB/cJ (BALB) mice, being lower in BALBs by about 10 mV (Ohlemiller et al. Hear Res 220: 10-26, 2006). This difference corresponds to strain differences with respect to the density of marginal cells in cochlear stria vascularis. After about 1 year of age, BALB mice also tend toward EP reduction that correlates with further marginal cell loss. We therefore suggested that early sub-clinical features of the BALB stria vascularis may predispose these mice to a condition modeling Schuknecht's strial presbycusis. We further reported (Ohlemiller et al. J Assoc Res Otolaryngol 12: 45-58, 2011) that the acute effects of a 2-h 110 dB SPL noise exposure differ between B6 and BALB mice, such that the EP remains unchanged in B6 mice, but is reduced by 40-50 mV in BALBs. In about 25 % of BALBs, the EP does not completely recover, so that permanent EP reduction may contribute to noise-induced permanent threshold shifts in BALBs. To identify genes and alleles that may promote natural EP variation as well as noiserelated EP reduction in BALB mice, we have mapped related quantitative trait loci (QTLs) using 12 recombinant inbred (RI) strains formed from B6 and BALB (CxB1-CxB12). EP and strial marginal cell density were measured in B6 mice, BALB mice, their F1 hybrids, and RI mice without noise exposure, and 1-3 h after broadband noise (4-45 kHz, 110 dB SPL, 2 h). For unexposed mice, the strain distribution patterns for EP and marginal cell density were used to generate preliminary QTL maps for both EP and marginal cell density. Six QTL regions were at least statistically suggestive, including a significant QTL for marginal cell density on chromosome 12 that overlapped a weak QTL for EP variation. This region, termed Maced (Marginal cell density QTL) supports the notion of marginal cell density as a genetically influenced contributor to natural EP variation. Candidate genes for Maced notably include Foxg1, Foxa1, Akap6, Nkx2-1, and Pax9. Noise exposure produced significant EP reductions in two RI strains as well as significant EP increases in two RI strains. QTL mapping of the EP in noise-exposed RI mice yielded four suggestive regions. Two of these overlapped with QTL regions we previously identified for noise-related EP reduction in CBA/J mice (Ohlemiller et al. Hear Res 260: 47-53, 2010) on chromosomes 5 and 18 (Nirep). The present map may narrow the Nirep interval to a~10-Mb region of proximal Chr. 18 that includes Zeb1, Arhgap12, Mpp7, and Gjd4. This study marks the first exploration of natural gene variants that modulate the EP. Their orthologs may underlie some human hearing loss that originates in the lateral wall.Electronic supplementary material The online version of this article

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“…The maintenance of the normal electrical polarization and ionic composition of the cochlear endolymph depends not only upon the active ionic transport mechanisms of stria vascularis, but also on the permeability properties of those cells which represent the endolymph-perilymph barrier (JAHNKE , 1975 ;SMITH, 1978). Studies by KONISHI et al (1979) and KONISHI and SALT (1980) have indicated that changes in the permeability of the endolymph-perilymph barrier to potassium ions may represent an important factor in the hearing loss produced by intermediate levels of noise or treatment with ototoxic antibiotics . BOSHER (1980) has also reported that one effect of ethacrynic acid on the cochlea is to decrease cation permeabilities of the endolymph system, primarily with respect to potassium ions.…”

mentioning

confidence: 99%

“…Studies by KONISHI et al (1979) and KONISHI and SALT (1980) have indicated that changes in the permeability of the endolymph-perilymph barrier to potassium ions may represent an important factor in the hearing loss produced by intermediate levels of noise or treatment with ototoxic antibiotics . BOSHER (1980) has also reported that one effect of ethacrynic acid on the cochlea is to decrease cation permeabilities of the endolymph system, primarily with respect to potassium ions.…”

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confidence: 99%

Functional importance of sodium and potassium in the guinea pig cochlea studied with amiloride and tetraethylammonium.

Salt

Konishi

1982

Jpn.J.Physiol

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The effects of amiloride on the cochlear responses in the guinea pig were compared with those produced by tetraethylammonium (TEA). Amiloride has been reported to reduce membrane permeability to sodium in a wide variety of ion-transporting epithelia. TEA has been documented to suppress the active potassium permeability increase during the repolarization phase of the action potential in mammalian excitable cells, and to reduce the resting potassium conductance in mammalian smooth muscle cells. Perilymphatic perfusion of 10-3 M amiloride or intravenous injection at a dose of 20 mg/kg suppressed the whole nerve action potential (AP) of the cochlea but did not significantly affect the cochlear microphonics (CM) or endocochlear potential (EP). Application of amiloride to endolymph by iontophoretic or perfusion techniques also produced no significant changes of CM and EP when compared with appropriate control procedures. Perilymphatic perfusion of 10-2 M TEA did not suppress CM or EP but the AP was reduced. Iontophoretic application of TEA to the endolymph caused a marked suppression of CM while the EP was significantly increased. The effects of endolymphatic TEA application are consistent with the concept that the normal EP recorded from scala media is the algebraic sum of a positive electrogenic potential and a negative diffusion potential, the latter component being sensitive to potassium permeability changes of the endolymph-perilymph barrier. Maintenance of normal cochlear microphonics also appears dependent upon the maintenance of normal potassium permeability properties of the endolymph-perilymph barrier. The functional importance of normal sodium permeability properties appears less certain. Key Words: sodium, potassium, cochlea, permeability. The maintenance of the normal electrical polarization and ionic composition of the cochlear endolymph depends not only upon the active ionic transport mechanisms of stria vascularis, but also on the permeability properties of those cells which represent the endolymph-perilymph barrier (JAHNKE , 1975 ;SMITH, 1978). Studies by KONISHI et al. (1979) and KONISHI and SALT (1980) have indicated that changes in the permeability of the endolymph-perilymph barrier to potassium ions may represent an important factor in the hearing loss produced by intermediate levels of noise or treatment with ototoxic antibiotics . BOSHER (1980) has also reported that one effect of ethacrynic acid on the cochlea is to decrease cation permeabilities of the endolymph system, primarily with respect to potassium ions.The present study has used amiloride and tetraethylammonium in an attempt to assess the relative importance of sodium and potassium ion movements to cochlear function. Amiloride is known as a potassium sparing diuretic that acts primarily upon the distal tubule in the mammalian kidney (DUARTE et al., 1971). Following studies in the toad bladder (BENTLEY , 1968;SUDOU and HOSHI, 1977), the renal tubule (STONER et al., 1974), and the submaxillary gland (SCHNEYER, 1970), there is a...

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Effects of exposure to noise on ion movement in guinea pig cochlea

Cited by 64 publications

References 22 publications

Age-Related Changes in Cochlear Endolymphatic Potassium and Potential in CD-1 and CBA/CaJ Mice

Age-Related Changes in Cochlear Endolymphatic Potassium and Potential in CD-1 and CBA/CaJ Mice

QTL Mapping of Endocochlear Potential Differences between C57BL/6J and BALB/cJ mice

Functional importance of sodium and potassium in the guinea pig cochlea studied with amiloride and tetraethylammonium.

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