Effects of extracellular Ca2+ on phagocytosis and intracellular Ca2+ concentrations in polymorphonuclear leukocytes of postpartum dairy cows

Ducusin, Rio John T.; Uzuka, Yuji; Satoh, Eiki; Otani, Masayuki; Nishimura, Maki; Tanabe, Shigeyuki; Sarashina, Takao

doi:10.1016/s0034-5288(03)00038-9

Cited by 46 publications

(45 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The association between decreased neutrophil function and SCH (Martinez et al, 2012) or clinical hypocalcemia (Ducusin et al, 2003) has been reported before. Cows with milk fever have been shown to have reduced cytosolic concentrations of iCa 2+ in PBMC, which has been suggested to impair immune function (Kimura et al, 2006).…”

supporting

confidence: 59%

“…The increased risk of developing these conditions is likely mediated by several factors, including the effects of low iCa 2+ suppressing smooth muscle contractions (Hansen et al, 2003). Furthermore, hypocalcemic cows have increased plasma concentrations of cortisol (Horst and Jorgensen, 1982), reduced proportion of neutrophils with phagocytic activity (Ducusin et al, 2003;Martinez et al, 2012), and impaired mononuclear cell response to an antigen-activating stimulus (Kimura et al, 2006). This reduction of immune response has linked hypocalcemia to infectious diseases of bacterial origin such as metritis (Martinez et al, 2012) and mastitis (Curtis et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows

Martínez

Sinedino

Bisinotto

et al. 2014

Journal of Dairy Science

194

201

View full text Add to dashboard Cite

The objectives were to study the effects of induced subclinical hypocalcemia [SCH, blood ionized Ca (iCa(2+)) <1.0mM, without recumbency] on physiological responses and function of immune cells in dairy cows. Ten nonpregnant, nonlactating Holstein cows were blocked by lactation and assigned randomly to a normocalcemic (NC; intravenous infusion of 0.9% NaCl i.v. plus 43 g of oral Ca, as Ca sulfate and Ca chloride, at -1 and 11h) or an induced SCH [SCHI, 5% ethylene glycol tetraacetic acid (EGTA), a selective iCa(2+) chelator, intravenous infusion] treatment for 24h, using a crossover design. The sequence of treatments was either NC-SCHI or SCHI-NC, with a 6-d washout period. Ionized Ca was evaluated before, hourly during the infusion period, and at 48 and 72 h, to monitor concentrations and adjust the rate of infusion, maintaining blood iCa(2+) <1.0mM in SCHI throughout the 24-h infusion period. Additional measurements included heart and respiratory rates, rectal temperature, dry matter intake, rumen contractions, whole-blood pH, concentrations of glucose and K in whole blood, concentrations of total Ca, Mg, nonesterified fatty acids, β-hydroxybutyrate, and insulin in plasma, and urinary excretion of Ca. Total and differential leukocyte count in blood was also performed. The concentration of cytosolic iCa(2+) in neutrophils and lymphocytes was quantified and neutrophil function was assayed in vitro. Infusion of a 5% EGTA solution successfully induced SCH in all SCHI cows, resulting in decreased blood iCa(2+) concentrations throughout the 24-h treatment period (0.77 ± 0.01 vs. 1.26 ± 0.01 mM iCa(2+)). Induction of SCH reduced dry matter intake on the day of infusion (5.3 ± 0.8 vs. 9.1 ± 0.8 kg/d) and rumen contractions (1.9 ± 0.2 vs. 2.7 ± 0.2 contractions/2 min) for the last 12h of infusion. Cows in SCHI had decreased plasma insulin concentration (1.44 ± 0.23 vs. 2.32 ± 0.23 ng/mL) evident between 6 and 18 h after the beginning of the infusion, accompanied by increased concentrations of glucose (4.40 ± 0.04 vs. 4.17 ± 0.04 mM). Plasma nonesterified fatty acids concentration was greater for SCHI than NC cows (0.110 ± 0.019 vs. 0.061 ± 0.014 mM). Neutrophils of cows in SCHI had a faster decrease in cytosolic iCa(2+) after stimulation with ionomycin (9.9 ± 1.0 vs. 13.6 ± 1.4 Fluo-4:Fura Red post-end ratio) in vitro. Furthermore, induction of SCH reduced the percentage of neutrophils undergoing phagocytosis (22.1 ± 2.1 vs. 29.3 ± 2.1%) and reduced the oxidative burst response after incubation of pathogenic bacteria (16.1 ± 1.7 vs. 24.2 ± 1.7%). Subclinical hypocalcemia compromised appetite, altered metabolism, and impaired function of immune cells in dairy cows.

show abstract

supporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows

Martínez

Sinedino

Bisinotto

et al. 2014

Journal of Dairy Science

194

201

View full text Add to dashboard Cite

show abstract

“…In particular, prior studies have used buffers without calcium to reduce the probability of neutrophil activation. Considering that our phagocytosis experiments take place in a buffer with calcium [otherwise, we found that bead uptake occurs at a low rate (Ducusin et al, 2003)] we felt it necessary to obtain data on passive cell deformation in the presence of this obviously important ion. Two kinds of experiments were performed: (1) static measurements of the cortical tension at steady state for various increases of cell surface area, and (2) dynamic measurements of the cortical tension under various rates of area expansion during aspiration.…”

Section: Passive Deformationmentioning

confidence: 99%

Mechanics of neutrophil phagocytosis: behavior of the cortical tension

Herant

Heinrich

Dembo

2005

Journal of Cell Science

145

189

View full text Add to dashboard Cite

The mechanical implementation of phagocytosis requires a well-coordinated deployment of cytoplasm and membrane during the creation of a phagosome. We follow the time course of this process in initially round passive neutrophils presented with antibody-coated beads of radii 1.1 to 5.5 μm. In particular, we monitor the cortical tension as the apparent cellular surface area increases due to cell-driven deformations induced by phagocytosis. The behavior of the tension is then compared with conditions of similar area expansion caused by externally imposed deformations during cell aspiration into a micropipette. Whereas the resting tension remains low for an area expansion of up to only 30% during aspiration, it remains low even after an area expansion of up to 80% in phagocytosis. This is probably the result of membrane insertion from inner stores by exocytosis. We further find that the onset of viscous tension, proportional to the rate of area expansion and caused by the unfurling of plasma membrane wrinkles, is significantly delayed in phagocytosis compared with aspiration. We propose that this is the result of phagocytosis-triggered enzymatic activity that releases spare plasma membrane normally sequestered by velcro-like bonds in a reservoir of surface folds and villi.

show abstract

“…Η υπασβεστιαιμία ελαττώνει τις συσπάσεις του μυομητρίου και δρα ανασταλτικά στη λΰση του κολλαγόνου, στην πρωτεόλυση και στη δραστηριότητα των ουδετερόφιλων λευκοκυττάρων, λειτουργίες που είναι απαραίτητες για την αποβολή των εμβρυϊκών υμένων (Brogley et al 1999, Ducusin et al 2003, Melendez et al 2006. Επιδημιολογικές μελέτες συνδέουν την υπασβεστιαιμία με την αυξημένη συχνότητα εμφάνισης κατακράτησης των εμβρυϊκών υμένων (KEY) (Curtis et al 1983).…”

Section: υπασβεστιαιμία και γεννητικό σύστημαunclassified

Managing the transition period of dairy cows to prevent major metabolic postpartum disorders

Brozos

Kalaitzakis²,

Panousis

2017

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

The transition period refers to the time between 3-4 weeks prior to and 4 weeks after calving. This is the period with the highest incidence of metabolic and other periparturient disorders. Many of these disorders interrelated and often coincide. Proper management and nutrition of the transition cow are critical for obtaining maximum dry matter intake, good health, increased reproductive efficiency and optimum milk production in the following lactation. The present review is comprised from two parts. In the first one, selected information is provided concerning the most important metabolic diseases of the transition period (hypocalcemia, ketosis) and, in brief, how hypocalcemia is interrelated to other diseases (mastitis, metritis, retained fetal membranes and abomasal displacement). In the second part, up-to-date nutritional methods applied in the transition period to prevent these metabolic periparturient diseases, with emphasis in hypocalcemia, are described.

show abstract

Effects of extracellular Ca2+ on phagocytosis and intracellular Ca2+ concentrations in polymorphonuclear leukocytes of postpartum dairy cows

Cited by 46 publications

References 20 publications

Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows

Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows

Mechanics of neutrophil phagocytosis: behavior of the cortical tension

Managing the transition period of dairy cows to prevent major metabolic postpartum disorders

Contact Info

Product

Resources

About