Effects of extracellular calcium concentration on neurite outgrowth in PC12 cells by staurosporine

Zhaleh, Hossein; Azadbakht, Mehri; Pour, Ali Bidmeshki

doi:10.1016/j.neulet.2011.04.020

Cited by 10 publications

(11 citation statements)

References 25 publications

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“…We reported previously that staurosporine could induce neurite outgrowth in PC12 cells and that this apoptosis can be blocked by reducing extracellular calcium stores (12). In contrast, present data suggest that staurosporine can induce apoptosis in cultured cortical neurons and that this apoptosis can be blocked by raising intracellular Ca 2+ (9).…”

contrasting

confidence: 70%

“…On the other hand, recent study showed that staurosporine leads to intracellular calcium overload, which induces apoptosis in PC12 cells (6). In the percent study, we showed that inhibition of IP3R and PLCγ caused a large increase in (Ca We reported previously that staurosporine can induce neurite outgrowth in PC12 cells and that this apoptosis can be blocked by reducing extracellular calcium stores (12). In addition, it is known that cytosolic Ca 2+ increase caused by staurosporine, which in turn mobilizes Ca 2+ from different sources, might cause apoptosis in astrocytes (27).…”

Section: Discussionmentioning

confidence: 67%

“…Hoechst / PI nuclear staining was carried out as previously described (12). Briefl y, cells were plated in 24-well culture plates with 10 4 cells/mL density for 12 h. Then cells were pretreated with different treatment mediums for certain time.…”

Section: Quantifi Cation Of Cell Death Incidencementioning

confidence: 99%

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Phosphoinositid signal pathway mediate neurite outgrowth in PC12 cells by staurosporine

Zhaleh¹,

Azadbakht²,

Pour³

2014

BLL

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Objectives:In previous studies, we showed that staurosporine uses intracellular calcium ions to affect cell death in PC12 cells. The bulk release of intracellular excessive Ca 2+ from intracellular sources into cytosol contributes to neuronal apoptotic events, which in turn results in neuronal cell death. However, the mechanisms of Ca 2+ -induced neuronal cell death or neurite elongation is still unclear. Therefore, we investigated the relation between phosphoinositid signal pathway, intracellular calcium, and reactive oxygen species on one hand, with staurosporine-induced neurite outgrowth in PC12 cells on the other. Results: The inhibition of phospholipase C or IP3 receptor antagonist or phosphoinositid signal transduction antagonist produced cell death and suppressed neurite outgrowth by staurosporine in PC12 cells. The inhibition of these enzymes and pathway results in an increase in intracellular Ca 2+ although subsequent hydroxyl radical (•OH) production began after inhibitors exposure.•OH production was signifi cantly attenuated in inhibitor supplemented medium treatment, and it was dependent on the intracellular Ca 2+ concentration. These data indicate that staurosporine activates phosphoinositid signal pathway while endoplasmic Ca 2+, and subsequent •OH production are critical events in staurosporine-induced neurite outgrowth in PC12 cells. Conclusion:We conclude that the fact that staurosporine mobilizes Ca2+ , probably via activating the subcellular compartment, is responsible for staurosporine-induced (Ca2+]i increase during neurite outgrowth in PC12 cells (Fig. 7, Ref. 30). Text in PDF www.elis.sk.

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confidence: 70%

Section: Discussionmentioning

confidence: 67%

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Phosphoinositid signal pathway mediate neurite outgrowth in PC12 cells by staurosporine

Zhaleh¹,

Azadbakht²,

Pour³

2014

BLL

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“…At this concentration, apoptotic and differentiated cells would be present, which is crucial for conducting this experiment. Cytotoxicity measurement of treatment, quantification of cell death incidence, measurement of total neurite length and fraction of cell differentiation were conducted in the previous study (Zhaleh et al, 2011). 2.2 | RNA extraction, quality assessment, sequencing, and data analysis Total RNAs were extracted using the RNeasy Mini kit (Qiagen, Hilden, Germany) according to the Qiagen RNA extraction protocol for animal cell lines. Quality of the total RNA samples were assessed by agarose gel electrophoresis using standard protocols.…”

Section: Cell Culture and Treatmentmentioning

confidence: 99%

Regulatory networks upon neurogenesis induction in PC12 cell line by small molecules

Falsafi

Soleimani

Fallahi

et al. 2019

Journal Cellular Physiology

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Alteration in the normal regulatory pathway of differentiation can lead to the induction of programmed cell death. Accordingly, some chemicals like staurosporine, nerve growth factor, pituitary adenylate cyclase activating peptide, and trimethyltin are shown to be able to induce differentiation in vitro, via different mechanisms in the PC12 cell line. Hence, understanding the details of the molecular mechanisms of differentiation induction by these small molecules are important for further application of these molecules in neurogenesis. Therefore, we sought to determine these signaling pathways, using gene regulatory networks analysis. Then, we have conducted a comparative analysis of the alterations in the gene expression pattern of the PC12 cell lines in response to these chemicals at the early stages. Based on the comparative analysis and previous knowledge, we have proposed the affected pathways during differentiation and apoptosis. Our findings could be useful in the development of protocols to reprogramming of neurons by such small molecules with high efficiency.

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“…, and calcium homeostasis regulation were related by mechanisms playing an important role in regulation of STS-induced cell death (65,66). Recently, we reported that inhibition of IP3R and PLCγ led to attenuating of the rise in intracellular calcium triggered by staurosporine, thus strongly suggesting that ROS generation could potentiate the observed intracellular calcium overload (67).…”

Section: +mentioning

confidence: 99%

Inhibitory effects of mouse bone marrow mesenchymal stem cell soup on staurospurine-induced cell death in MCF-7 and AGS

Zhaleh¹,

Azadbakht²,

Pour³

2017

BLL

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BACKGROUND: Staurospurine induces apoptosis in cell line. Bone Marrow Mesenchymal stem cells Soup is a promising tool for cell proliferation via a variety of secreted factors. In this study, we examined the effects of BMSCs Soup on Staurospurine induced-cell death in MCF-7 and AGS cells. METHODS: There were three Groups: Group I: no incubation with BM Soup; Group II: incubated with 24 h BM Soup; Group III: incubation with 48 h BM Soup. There were two treatments in each group. The treatments were 1μM Staurospurine (Treatment 1) and 0.0 μM Staurospurine (Treatment 2). The cells were cultured in culture medium containing 0.2 % BSA. We obtained the cell viability, cell death and NO concentration. RESULTS: Our results showed that BM soup administration for 48 hours protectsed against 1μM staurosporine concentration induced cell death and reduced cell toxicity in MCF-7 and AGS cells. Cell viability and cell toxicity assay showed that BM soup in time dependent manner increased cell viability (p < 0.05) and cell death assay showed that cell death in time dependent manner was decreased(p < 0.05). Our data showed that BM soup with increasing NO concentration reduced staurospurine induced cell death and cell cytotoxicity (p < 0.05). CONCLUSION: It's concluded that BMSCs soup suppressed staurospurine-induced cytotoxicity activity process in MCF-7 and AGS cells (Fig. 9, Ref. 79). Text in PDF www.elis.sk.

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Effects of extracellular calcium concentration on neurite outgrowth in PC12 cells by staurosporine

Cited by 10 publications

References 25 publications

Phosphoinositid signal pathway mediate neurite outgrowth in PC12 cells by staurosporine

Phosphoinositid signal pathway mediate neurite outgrowth in PC12 cells by staurosporine

Regulatory networks upon neurogenesis induction in PC12 cell line by small molecules

Inhibitory effects of mouse bone marrow mesenchymal stem cell soup on staurospurine-induced cell death in MCF-7 and AGS

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