Effects of extracellular lactate on production of reactive oxygen species by equine polymorphonuclear leukocytes in vitro

Echigoya, Yusuke; Morita, Shohei; Itou, Takuya; Sakai, Takeo

doi:10.2460/ajvr.73.8.1290

Cited by 12 publications

(6 citation statements)

References 36 publications

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“…When energy consumption in the tissue increased sharply during the exhaustive training, mitochondrial oxygen consumption also increased, and ROS generation rose. Lactic acid concentration increases caused by exhaustive training induce a synergistic effect on ROS production [24]. …”

Section: Discussionmentioning

confidence: 99%

Exhaustive Training Increases Uncoupling Protein 2 Expression and Decreases Bcl-2/Bax Ratio in Rat Skeletal Muscle

Liu

2013

Oxidative Medicine and Cellular Longevity

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This work investigates the effects of oxidative stress due to exhaustive training on uncoupling protein 2 (UCP2) and Bcl-2/Bax in rat skeletal muscles. A total of 18 Sprague-Dawley female rats were randomly divided into three groups: the control group (CON), the trained control group (TC), and the exhaustive trained group (ET). Malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD), ATPase, UCP2, and Bcl-2/Bax ratio in red gastrocnemius muscles were measured. Exhaustive training induced ROS increase in red gastrocnemius muscles, which led to a decrease in the cell antiapoptotic ability (Bcl-2/Bax ratio). An increase in UCP2 expression can reduce ROS production and affect mitochondrial energy production. Thus, oxidative stress plays a significant role in overtraining.

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Section: Discussionmentioning

confidence: 99%

Exhaustive Training Increases Uncoupling Protein 2 Expression and Decreases Bcl-2/Bax Ratio in Rat Skeletal Muscle

Liu

2013

Oxidative Medicine and Cellular Longevity

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“…107 For example, cancer and stromal cells secrete lactate, and extracellular lactate directly increases ROS levels in neutrophils, CD34+ vasculogenic stem cells, and cancer cells. 108, 109 …”

Section: Targeting Metabolic Synergy Between Cancer and Stromal Cellsmentioning

confidence: 99%

ROS homeostasis and metabolism: a critical liaison for cancer therapy

Kim

Bae³

2016

Exp Mol Med

244

147

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Evidence indicates that hypoxia and oxidative stress can control metabolic reprogramming of cancer cells and other cells in tumor microenvironments and that the reprogrammed metabolic pathways in cancer tissue can also alter the redox balance. Thus, important steps toward developing novel cancer therapy approaches would be to identify and modulate critical biochemical nodes that are deregulated in cancer metabolism and determine if the therapeutic efficiency can be influenced by changes in redox homeostasis in cancer tissues. In this review, we will explore the molecular mechanisms responsible for the metabolic reprogramming of tumor microenvironments, the functional modulation of which may disrupt the effects of or may be disrupted by redox homeostasis modulating cancer therapy.

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“…Previously, ROS production by l ‐lactic acid has been reported in human leukocytes and Drosophila intestinal cells (Echigoya et al , ; Iatsenko et al , ). l ‐Lactic acid triggered NADPH oxidase (Nox)‐mediated ROS production through L‐lactate oxidation by the mitochondrial L‐LDH in Drosophila intestinal cell (Iatsenko et al , ).…”

Section: Discussionmentioning

confidence: 97%

d‐Lactic acid secreted by Chlorella fusca primes pattern‐triggered immunity against Pseudomonas syringae in Arabidopsis

et al. 2020

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Biological control agents including microbes and their products have been studied as sustainable crop protection strategies. Although aquatic microalgae have been recently introduced as a biological control agent, the underlying molecular mechanisms are largely unknown. The aim of the present study was to investigate the molecular mechanisms underlying biological control by microalga Chlorella fusca. Foliar application of C. fusca elicits induced resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000 that activates plant immunity rather than direct antagonism. To understand the basis of C. fuscatriggered induced resistance at the transcriptional level, we conducted RNA sequencing (RNA-seq) analysis. RNA-seq data showed that, upon pathogen inoculation, C. fusca treatment primed the expression of cysteine-rich receptor-like kinases, WRKY transcription factor genes, and salicylic acid and jasmonic acid signalling-related genes. Intriguingly, the application of C. fusca primed pathogen-associated molecular pattern -triggered immunity, characterized by reactive oxygen species burst and callose deposition, upon flagellin 22 treatment. The attempts to find C. fusca determinants allowed us to identify D-lactic acid secreted in the supernatant of C. fusca as a defence priming agent. This is the first report of the mechanism of innate immune activation by aquatic microalga Chlorella in higher plants.

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Effects of extracellular lactate on production of reactive oxygen species by equine polymorphonuclear leukocytes in vitro

Abstract: Extracellular lactate potentially has a direct effect on the capacity to produce ROS by equine PMNs, which may be associated with alterations in innate immune functions within a short period after high-intensity exercise.

Cited by 12 publications

References 36 publications

Exhaustive Training Increases Uncoupling Protein 2 Expression and Decreases Bcl-2/Bax Ratio in Rat Skeletal Muscle

Exhaustive Training Increases Uncoupling Protein 2 Expression and Decreases Bcl-2/Bax Ratio in Rat Skeletal Muscle

ROS homeostasis and metabolism: a critical liaison for cancer therapy

d‐Lactic acid secreted by Chlorella fusca primes pattern‐triggered immunity against Pseudomonas syringae in Arabidopsis

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