Effects of Extraction Technique on the Content and Antioxidant Activity of Flavonoids from Gossypium Hirsutum linn. Flowers

Abstract

Cotton is one of the Uyghur medical materials in China and is rich in flavonoids. Flavonoids have important pharmacological effects. The yield of flavonoids in traditional extraction methods is low, which affects the development of flavonoids. Therefore, it is urgent to optimize the extraction techniques. The yield of flavonoids in cotton flowers was effectively improved by response surface methodology, and the highest yield of flavonoids reached 5.66%, and the optimal extraction process conditions were obtained. The DPPH free radical scavenging rate, hydroxyl free radical scavenging rate, superoxide anion free radical scavenging rate, and reducing ability were tested to reflect the antioxidant capacity of flavonoids. The flavonoids had an excellent antioxidant effect. Cell experiments suggested that the flavonoids had the effect of protecting glutamate-induced damage to HT-22 cells. The results of this study provide a theoretical basis for the extraction of cotton flowers flavonoids and the comprehensive evaluation of antioxidant products, as well as the extraction of other plant flavonoids.

Effect of ethanol solvent concentrations in pepino melon fruit (Solanum muricatum Aiton) extraction on total flavonoid, phenolic, and beta-carotene content

“…The extract with 70% ethanol had the highest total flavonoid value (0.559 ± 0.03 mgQE/g extract). The same results are from the previous research which gave the highest total flavonoids in 70% ethanol (Suhendra et al, 2019;Rahardhian et al, 2019;Dong et al, 2022). Sucipto et al (2022) reported that optimization results of ethanol concentration showed that 70% ethanol was optimal for extracting flavonoids.…”

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“…Previous research has shown that hydroxyl radicals, which are produced naturally, are highly reactive free radicals, and that humans lack enzymes that can neutralize them [36]. Previous research established that hydroxyl radicals are highly reactive free radicals produced in the biological system, and that human ROS lacks enzymes specifically designed to neutralize them [37]. The highly high reactivity of hydroxyl (OH) radical makes its scavenging a crucial antioxidant activity, as was shown in a previous work [38].…”

Antioxidant Activity of Total Flavonoids from Persicaria hydropiper (L.) Spach and Their Bacteriostatic Effect on Pathogenic Bacteria of Broiler Origin

“…The antioxidant activity of TFs-Ph was evaluated by referring to some literature with minor modifications, including the scavenging activity of •OH, DPPH, •O 2− , and ABTS [29][30][31].…”

“…This is because an appropriate increase in temperature can accelerate the diffusion rate of molecules, and thus increase the release of bioactive substances in plant cells. But when the temperature is higher than the optimal level it would lead to the decomposition of heat-sensitive compounds such as flavonoids [29]. Thus, the best heat reflux temperature was 70 • C.…”